Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2302 and Dsui_2303 are separated by 39 nucleotides Dsui_2303 and Dsui_2304 are separated by 29 nucleotides Dsui_2304 and Dsui_2305 overlap by 1 nucleotides Dsui_2305 and Dsui_2306 are separated by 200 nucleotides
Dsui_2302: Dsui_2302 - acyl-CoA dehydrogenase, at 2,458,532 to 2,460,325
_2302
Dsui_2303: Dsui_2303 - putative membrane protein, at 2,460,365 to 2,461,030
_2303
Dsui_2304: Dsui_2304 - electron transfer flavoprotein, alpha subunit, at 2,461,060 to 2,461,989
_2304
Dsui_2305: Dsui_2305 - electron transfer flavoprotein, beta subunit, at 2,461,989 to 2,462,738
_2305
Dsui_2306: Dsui_2306 - transcriptional regulator, at 2,462,939 to 2,463,523
_2306
Position (kb)
2461
2462 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2460.127 kb on + strand, within Dsui_2302 at 2460.184 kb on + strand at 2460.193 kb on - strand at 2460.193 kb on - strand at 2460.232 kb on - strand at 2460.259 kb on + strand at 2460.267 kb on - strand at 2460.267 kb on - strand at 2460.322 kb on - strand at 2460.342 kb on + strand at 2460.407 kb on - strand at 2460.424 kb on + strand at 2460.636 kb on - strand, within Dsui_2303 at 2460.687 kb on + strand, within Dsui_2303 at 2460.687 kb on + strand, within Dsui_2303 at 2460.776 kb on + strand, within Dsui_2303 at 2460.781 kb on + strand, within Dsui_2303 at 2460.908 kb on - strand, within Dsui_2303 at 2460.992 kb on + strand at 2461.000 kb on - strand at 2461.000 kb on - strand at 2461.005 kb on - strand at 2461.057 kb on + strand at 2461.177 kb on - strand, within Dsui_2304 at 2461.384 kb on + strand, within Dsui_2304 at 2461.454 kb on - strand, within Dsui_2304 at 2461.503 kb on - strand, within Dsui_2304 at 2461.561 kb on - strand, within Dsui_2304 at 2461.588 kb on + strand at 2462.045 kb on + strand at 2462.061 kb on - strand at 2462.089 kb on - strand, within Dsui_2305 at 2462.210 kb on - strand, within Dsui_2305 at 2462.272 kb on - strand, within Dsui_2305 at 2462.311 kb on - strand, within Dsui_2305 at 2462.439 kb on - strand, within Dsui_2305 at 2462.458 kb on - strand, within Dsui_2305 at 2462.690 kb on + strand at 2462.747 kb on + strand at 2462.747 kb on + strand at 2462.755 kb on - strand at 2462.755 kb on - strand at 2462.755 kb on - strand at 2462.755 kb on - strand at 2462.755 kb on - strand at 2462.788 kb on + strand at 2462.796 kb on - strand at 2462.806 kb on + strand at 2462.814 kb on - strand at 2462.814 kb on - strand at 2462.820 kb on + strand at 2462.828 kb on - strand at 2462.830 kb on + strand at 2462.838 kb on - strand at 2462.870 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 2,460,127 + Dsui_2302 0.89 -1.1 2,460,184 + -0.7 2,460,193 - -0.4 2,460,193 - +0.2 2,460,232 - -0.3 2,460,259 + +0.0 2,460,267 - -0.0 2,460,267 - +0.7 2,460,322 - -0.5 2,460,342 + -1.3 2,460,407 - +0.0 2,460,424 + +0.6 2,460,636 - Dsui_2303 0.41 -0.2 2,460,687 + Dsui_2303 0.48 -1.4 2,460,687 + Dsui_2303 0.48 +0.8 2,460,776 + Dsui_2303 0.62 -0.9 2,460,781 + Dsui_2303 0.62 +0.6 2,460,908 - Dsui_2303 0.82 -0.4 2,460,992 + +0.1 2,461,000 - +0.0 2,461,000 - -0.2 2,461,005 - -0.4 2,461,057 + +0.2 2,461,177 - Dsui_2304 0.13 -0.6 2,461,384 + Dsui_2304 0.35 +0.0 2,461,454 - Dsui_2304 0.42 -2.2 2,461,503 - Dsui_2304 0.48 -1.1 2,461,561 - Dsui_2304 0.54 +0.1 2,461,588 + +1.0 2,462,045 + +0.7 2,462,061 - -1.9 2,462,089 - Dsui_2305 0.13 -0.1 2,462,210 - Dsui_2305 0.29 -0.8 2,462,272 - Dsui_2305 0.38 -1.4 2,462,311 - Dsui_2305 0.43 -0.9 2,462,439 - Dsui_2305 0.60 -1.0 2,462,458 - Dsui_2305 0.63 -1.7 2,462,690 + -0.0 2,462,747 + +2.0 2,462,747 + -0.0 2,462,755 - -2.2 2,462,755 - -0.9 2,462,755 - -1.3 2,462,755 - -0.2 2,462,755 - -0.2 2,462,788 + -1.1 2,462,796 - -0.4 2,462,806 + +0.9 2,462,814 - -0.1 2,462,814 - +0.2 2,462,820 + +0.8 2,462,828 - +0.4 2,462,830 + +1.0 2,462,838 - +0.1 2,462,870 - -0.4
Or see this region's nucleotide sequence