Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2062 and Dsui_2063 are separated by 91 nucleotides Dsui_2063 and Dsui_2064 are separated by 135 nucleotides Dsui_2064 and Dsui_2065 are separated by 46 nucleotides
Dsui_2062: Dsui_2062 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 2,181,624 to 2,182,031
_2062
Dsui_2063: Dsui_2063 - protein chain release factor B, at 2,182,123 to 2,182,521
_2063
Dsui_2064: Dsui_2064 - ABC-type metal ion transport system, periplasmic component/surface antigen, at 2,182,657 to 2,183,466
_2064
Dsui_2065: Dsui_2065 - hypothetical protein, contains double-stranded beta-helix domain, at 2,183,513 to 2,184,088
_2065
Position (kb)
2182
2183 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2181.239 kb on + strand at 2181.247 kb on - strand at 2181.271 kb on + strand at 2181.281 kb on + strand at 2181.303 kb on + strand at 2181.479 kb on + strand at 2181.479 kb on + strand at 2181.487 kb on - strand at 2181.501 kb on - strand at 2181.569 kb on - strand at 2181.709 kb on + strand, within Dsui_2062 at 2181.709 kb on + strand, within Dsui_2062 at 2181.782 kb on - strand, within Dsui_2062 at 2181.785 kb on + strand, within Dsui_2062 at 2181.826 kb on + strand, within Dsui_2062 at 2181.850 kb on + strand, within Dsui_2062 at 2181.875 kb on + strand, within Dsui_2062 at 2181.921 kb on + strand, within Dsui_2062 at 2181.921 kb on + strand, within Dsui_2062 at 2181.983 kb on - strand, within Dsui_2062 at 2182.011 kb on - strand at 2182.013 kb on + strand at 2182.021 kb on - strand at 2182.021 kb on - strand at 2182.163 kb on + strand, within Dsui_2063 at 2182.293 kb on + strand, within Dsui_2063 at 2182.376 kb on + strand, within Dsui_2063 at 2182.384 kb on - strand, within Dsui_2063 at 2182.428 kb on - strand, within Dsui_2063 at 2182.503 kb on + strand at 2182.511 kb on - strand at 2182.516 kb on + strand at 2182.516 kb on + strand at 2182.523 kb on + strand at 2182.567 kb on - strand at 2182.636 kb on - strand at 2182.759 kb on - strand, within Dsui_2064 at 2182.792 kb on - strand, within Dsui_2064 at 2182.847 kb on - strand, within Dsui_2064 at 2182.847 kb on - strand, within Dsui_2064 at 2182.901 kb on - strand, within Dsui_2064 at 2182.909 kb on - strand, within Dsui_2064 at 2182.976 kb on + strand, within Dsui_2064 at 2183.011 kb on - strand, within Dsui_2064 at 2183.060 kb on + strand at 2183.070 kb on + strand, within Dsui_2064 at 2183.166 kb on + strand at 2183.167 kb on + strand at 2183.175 kb on + strand at 2183.255 kb on - strand, within Dsui_2064 at 2183.255 kb on - strand, within Dsui_2064 at 2183.339 kb on - strand, within Dsui_2064 at 2183.411 kb on + strand at 2183.421 kb on + strand at 2183.488 kb on + strand at 2183.506 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 2,181,239 + +0.5 2,181,247 - +0.0 2,181,271 + -0.0 2,181,281 + +0.3 2,181,303 + +1.8 2,181,479 + -0.5 2,181,479 + +2.1 2,181,487 - +1.5 2,181,501 - +0.1 2,181,569 - -0.9 2,181,709 + Dsui_2062 0.21 -0.1 2,181,709 + Dsui_2062 0.21 +0.4 2,181,782 - Dsui_2062 0.39 -0.4 2,181,785 + Dsui_2062 0.39 -0.6 2,181,826 + Dsui_2062 0.50 +0.3 2,181,850 + Dsui_2062 0.55 -0.8 2,181,875 + Dsui_2062 0.62 +0.0 2,181,921 + Dsui_2062 0.73 -0.4 2,181,921 + Dsui_2062 0.73 -0.2 2,181,983 - Dsui_2062 0.88 +0.2 2,182,011 - -0.2 2,182,013 + +0.2 2,182,021 - +0.4 2,182,021 - +0.9 2,182,163 + Dsui_2063 0.10 +0.1 2,182,293 + Dsui_2063 0.43 +0.0 2,182,376 + Dsui_2063 0.63 +0.5 2,182,384 - Dsui_2063 0.65 -0.2 2,182,428 - Dsui_2063 0.76 +1.2 2,182,503 + -0.3 2,182,511 - +2.1 2,182,516 + +0.4 2,182,516 + +0.1 2,182,523 + -0.5 2,182,567 - +1.1 2,182,636 - +0.6 2,182,759 - Dsui_2064 0.13 -0.8 2,182,792 - Dsui_2064 0.17 +0.9 2,182,847 - Dsui_2064 0.23 +0.2 2,182,847 - Dsui_2064 0.23 -0.4 2,182,901 - Dsui_2064 0.30 -0.3 2,182,909 - Dsui_2064 0.31 +0.2 2,182,976 + Dsui_2064 0.39 +1.7 2,183,011 - Dsui_2064 0.44 -0.9 2,183,060 + +0.8 2,183,070 + Dsui_2064 0.51 +0.8 2,183,166 + -0.2 2,183,167 + +2.1 2,183,175 + +1.3 2,183,255 - Dsui_2064 0.74 -0.7 2,183,255 - Dsui_2064 0.74 -0.4 2,183,339 - Dsui_2064 0.84 -0.4 2,183,411 + -0.7 2,183,421 + +0.9 2,183,488 + +3.1 2,183,506 - +0.8
Or see this region's nucleotide sequence