Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1988 and Dsui_1989 are separated by 135 nucleotides Dsui_1989 and Dsui_1990 are separated by 73 nucleotides Dsui_1990 and Dsui_1991 overlap by 35 nucleotides Dsui_1991 and Dsui_1992 are separated by 15 nucleotides Dsui_1992 and Dsui_1993 are separated by 94 nucleotides
Dsui_1988: Dsui_1988 - transcriptional regulator, at 2,097,554 to 2,098,528
_1988
Dsui_1989: Dsui_1989 - ribosomal protein S6, at 2,098,664 to 2,099,047
_1989
Dsui_1990: Dsui_1990 - primosomal replication protein N, at 2,099,121 to 2,099,411
_1990
Dsui_1991: Dsui_1991 - ribosomal protein S18, at 2,099,377 to 2,099,655
_1991
Dsui_1992: Dsui_1992 - ribosomal protein L9, at 2,099,671 to 2,100,120
_1992
Dsui_1993: Dsui_1993 - replicative DNA helicase, at 2,100,215 to 2,101,696
_1993
Position (kb)
2099
2100 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.522 kb on + strand at 2098.530 kb on - strand at 2098.530 kb on - strand at 2098.530 kb on - strand at 2099.769 kb on + strand, within Dsui_1992 at 2099.891 kb on + strand, within Dsui_1992 at 2100.163 kb on + strand at 2100.218 kb on - strand at 2100.228 kb on + strand at 2100.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 2,098,522 + +0.7 2,098,522 + +0.3 2,098,522 + +0.3 2,098,522 + -0.3 2,098,522 + -0.4 2,098,530 - +0.2 2,098,530 - +1.4 2,098,530 - +2.1 2,099,769 + Dsui_1992 0.22 -1.4 2,099,891 + Dsui_1992 0.49 -2.7 2,100,163 + +0.8 2,100,218 - -0.1 2,100,228 + +0.4 2,100,228 + +0.1
Or see this region's nucleotide sequence