Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1955 and Dsui_1956 overlap by 4 nucleotides Dsui_1956 and Dsui_1957 are separated by 83 nucleotides Dsui_1957 and Dsui_1958 are separated by 152 nucleotides Dsui_1958 and Dsui_1959 are separated by 94 nucleotides Dsui_1959 and Dsui_1960 are separated by 109 nucleotides Dsui_1960 and Dsui_1961 are separated by 15 nucleotides
Dsui_1955: Dsui_1955 - 3'-5' exonuclease, at 2,066,797 to 2,067,594
_1955
Dsui_1956: Dsui_1956 - Rhodanese-related sulfurtransferase, at 2,067,591 to 2,068,034
_1956
Dsui_1957: Dsui_1957 - tRNA-Val, at 2,068,118 to 2,068,194
_1957
Dsui_1958: Dsui_1958 - threonyl-tRNA synthetase, at 2,068,347 to 2,070,263
_1958
Dsui_1959: Dsui_1959 - translation initiation factor IF-3, at 2,070,358 to 2,070,837
_1959
Dsui_1960: Dsui_1960 - ribosomal protein L35, at 2,070,947 to 2,071,144
_1960
Dsui_1961: Dsui_1961 - ribosomal protein L20, at 2,071,160 to 2,071,519
_1961
Position (kb)
2068
2069
2070
2071 Strain fitness (log2 ratio)
-2
-1
0
1 at 2067.351 kb on + strand, within Dsui_1955 at 2067.432 kb on + strand, within Dsui_1955 at 2067.437 kb on + strand, within Dsui_1955 at 2067.444 kb on + strand, within Dsui_1955 at 2067.476 kb on - strand, within Dsui_1955 at 2067.643 kb on + strand, within Dsui_1956 at 2067.651 kb on - strand at 2067.651 kb on - strand, within Dsui_1956 at 2067.682 kb on + strand, within Dsui_1956 at 2067.715 kb on + strand, within Dsui_1956 at 2067.723 kb on + strand, within Dsui_1956 at 2067.768 kb on - strand, within Dsui_1956 at 2067.811 kb on + strand, within Dsui_1956 at 2067.821 kb on + strand, within Dsui_1956 at 2067.821 kb on + strand, within Dsui_1956 at 2067.829 kb on - strand, within Dsui_1956 at 2067.829 kb on - strand, within Dsui_1956 at 2067.929 kb on + strand, within Dsui_1956 at 2067.937 kb on - strand, within Dsui_1956 at 2068.012 kb on - strand at 2068.066 kb on + strand at 2068.074 kb on - strand at 2068.074 kb on - strand at 2068.074 kb on - strand at 2068.207 kb on + strand at 2068.209 kb on + strand at 2068.253 kb on + strand at 2068.325 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 2,067,351 + Dsui_1955 0.69 -1.1 2,067,432 + Dsui_1955 0.80 +0.5 2,067,437 + Dsui_1955 0.80 -2.1 2,067,444 + Dsui_1955 0.81 -0.8 2,067,476 - Dsui_1955 0.85 -0.7 2,067,643 + Dsui_1956 0.12 -0.2 2,067,651 - +0.5 2,067,651 - Dsui_1956 0.14 +0.9 2,067,682 + Dsui_1956 0.20 +0.1 2,067,715 + Dsui_1956 0.28 +0.1 2,067,723 + Dsui_1956 0.30 +0.1 2,067,768 - Dsui_1956 0.40 +0.9 2,067,811 + Dsui_1956 0.50 +0.4 2,067,821 + Dsui_1956 0.52 +0.2 2,067,821 + Dsui_1956 0.52 -0.1 2,067,829 - Dsui_1956 0.54 +1.2 2,067,829 - Dsui_1956 0.54 -0.5 2,067,929 + Dsui_1956 0.76 +0.0 2,067,937 - Dsui_1956 0.78 +0.9 2,068,012 - -0.9 2,068,066 + +0.1 2,068,074 - -0.5 2,068,074 - -0.7 2,068,074 - +0.1 2,068,207 + +0.1 2,068,209 + -0.2 2,068,253 + +0.1 2,068,325 + +0.2
Or see this region's nucleotide sequence