Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0016 and Dsui_0017 are separated by 139 nucleotides Dsui_0017 and Dsui_0018 overlap by 1 nucleotides Dsui_0018 and Dsui_0019 are separated by 52 nucleotides
Dsui_0016: Dsui_0016 - transcriptional regulator, at 13,983 to 14,891
_0016
Dsui_0017: Dsui_0017 - 2-haloalkanoic acid dehalogenase, type II, at 15,031 to 15,720
_0017
Dsui_0018: Dsui_0018 - putative permease, at 15,720 to 16,505
_0018
Dsui_0019: Dsui_0019 - malate synthase A, at 16,558 to 18,156
_0019
Position (kb) 15
16
17 Strain fitness (log2 ratio) -1
0
1 at 14.769 kb on + strand at 14.776 kb on - strand, within Dsui_0016 at 14.776 kb on - strand, within Dsui_0016 at 14.866 kb on + strand at 14.866 kb on + strand at 15.009 kb on + strand at 15.017 kb on - strand at 15.046 kb on - strand at 15.048 kb on + strand at 15.092 kb on - strand at 15.215 kb on - strand, within Dsui_0017 at 15.218 kb on + strand, within Dsui_0017 at 15.239 kb on + strand, within Dsui_0017 at 15.246 kb on - strand, within Dsui_0017 at 15.299 kb on - strand at 15.319 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.365 kb on + strand, within Dsui_0017 at 15.373 kb on - strand, within Dsui_0017 at 15.373 kb on - strand, within Dsui_0017 at 15.384 kb on - strand, within Dsui_0017 at 15.398 kb on + strand, within Dsui_0017 at 15.510 kb on - strand, within Dsui_0017 at 15.513 kb on + strand, within Dsui_0017 at 15.535 kb on - strand, within Dsui_0017 at 15.553 kb on - strand, within Dsui_0017 at 15.654 kb on + strand at 15.667 kb on - strand at 15.692 kb on - strand at 15.918 kb on - strand, within Dsui_0018 at 15.931 kb on + strand, within Dsui_0018 at 15.940 kb on - strand, within Dsui_0018 at 16.033 kb on - strand, within Dsui_0018 at 16.276 kb on - strand at 16.319 kb on - strand, within Dsui_0018 at 16.359 kb on - strand, within Dsui_0018 at 16.448 kb on + strand at 16.467 kb on + strand at 16.487 kb on + strand at 16.544 kb on + strand at 16.544 kb on + strand at 16.548 kb on + strand at 16.552 kb on - strand at 16.562 kb on + strand at 16.562 kb on + strand at 16.564 kb on - strand at 16.570 kb on - strand at 16.595 kb on + strand at 16.659 kb on + strand at 16.695 kb on - strand at 16.788 kb on - strand, within Dsui_0019 at 16.850 kb on - strand, within Dsui_0019 at 16.919 kb on + strand, within Dsui_0019 at 16.950 kb on + strand, within Dsui_0019 at 17.046 kb on - strand, within Dsui_0019 at 17.065 kb on - strand at 17.276 kb on + strand, within Dsui_0019 at 17.284 kb on - strand, within Dsui_0019 at 17.303 kb on - strand, within Dsui_0019 at 17.336 kb on + strand, within Dsui_0019 at 17.432 kb on + strand, within Dsui_0019 at 17.480 kb on + strand, within Dsui_0019 at 17.488 kb on - strand, within Dsui_0019 at 17.488 kb on - strand, within Dsui_0019
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 14,769 + +1.0 14,776 - Dsui_0016 0.87 +0.5 14,776 - Dsui_0016 0.87 +1.7 14,866 + -1.4 14,866 + +1.0 15,009 + +0.0 15,017 - +0.2 15,046 - -0.3 15,048 + -0.1 15,092 - +0.2 15,215 - Dsui_0017 0.27 -0.2 15,218 + Dsui_0017 0.27 -0.1 15,239 + Dsui_0017 0.30 +0.7 15,246 - Dsui_0017 0.31 +0.0 15,299 - +1.3 15,319 + Dsui_0017 0.42 -0.5 15,365 + Dsui_0017 0.48 +0.2 15,365 + Dsui_0017 0.48 -0.5 15,365 + Dsui_0017 0.48 -0.2 15,365 + Dsui_0017 0.48 +0.1 15,373 - Dsui_0017 0.50 +0.9 15,373 - Dsui_0017 0.50 +0.0 15,384 - Dsui_0017 0.51 -0.2 15,398 + Dsui_0017 0.53 -0.2 15,510 - Dsui_0017 0.69 +0.5 15,513 + Dsui_0017 0.70 -0.0 15,535 - Dsui_0017 0.73 -0.3 15,553 - Dsui_0017 0.76 -0.3 15,654 + +0.2 15,667 - +0.8 15,692 - +0.0 15,918 - Dsui_0018 0.25 +0.6 15,931 + Dsui_0018 0.27 -0.1 15,940 - Dsui_0018 0.28 -1.1 16,033 - Dsui_0018 0.40 -0.3 16,276 - -0.5 16,319 - Dsui_0018 0.76 -1.7 16,359 - Dsui_0018 0.81 -0.8 16,448 + +0.5 16,467 + -0.3 16,487 + +1.2 16,544 + +0.4 16,544 + -0.3 16,548 + +0.3 16,552 - -0.5 16,562 + +0.2 16,562 + -0.4 16,564 - +0.5 16,570 - -0.5 16,595 + +0.0 16,659 + +0.7 16,695 - +1.0 16,788 - Dsui_0019 0.14 +0.0 16,850 - Dsui_0019 0.18 +1.0 16,919 + Dsui_0019 0.23 +0.9 16,950 + Dsui_0019 0.25 -0.1 17,046 - Dsui_0019 0.31 -1.5 17,065 - +0.8 17,276 + Dsui_0019 0.45 +0.9 17,284 - Dsui_0019 0.45 +0.9 17,303 - Dsui_0019 0.47 -1.0 17,336 + Dsui_0019 0.49 +0.7 17,432 + Dsui_0019 0.55 -0.3 17,480 + Dsui_0019 0.58 +0.2 17,488 - Dsui_0019 0.58 +0.7 17,488 - Dsui_0019 0.58 +0.0
Or see this region's nucleotide sequence