Experiment: MoYLS4 with Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2030 and DvMF_2031 are separated by 586 nucleotides DvMF_2031 and DvMF_2032 are separated by 143 nucleotides DvMF_2032 and DvMF_2033 overlap by 1 nucleotides
DvMF_2030: DvMF_2030 - MerR family transcriptional regulator (RefSeq), at 2,514,765 to 2,515,484
_2030
DvMF_2031: DvMF_2031 - ribonucleotide-diphosphate reductase subunit alpha (RefSeq), at 2,516,071 to 2,518,383
_2031
DvMF_2032: DvMF_2032 - cell division ATP-binding protein FtsE (RefSeq), at 2,518,527 to 2,519,369
_2032
DvMF_2033: DvMF_2033 - protein of unknown function DUF214 (RefSeq), at 2,519,369 to 2,520,271
_2033
Position (kb)
2516
2517
2518
2519 Strain fitness (log2 ratio)
-1
0
1 at 2515.197 kb on + strand, within DvMF_2030 at 2515.212 kb on - strand, within DvMF_2030 at 2515.432 kb on + strand at 2515.595 kb on + strand at 2515.901 kb on + strand at 2516.004 kb on - strand at 2516.130 kb on - strand at 2516.140 kb on - strand at 2516.226 kb on + strand at 2516.234 kb on - strand at 2516.296 kb on + strand at 2516.351 kb on + strand, within DvMF_2031 at 2516.359 kb on - strand, within DvMF_2031 at 2516.397 kb on - strand, within DvMF_2031 at 2516.399 kb on + strand, within DvMF_2031 at 2516.482 kb on + strand, within DvMF_2031 at 2516.519 kb on - strand, within DvMF_2031 at 2516.543 kb on - strand, within DvMF_2031 at 2516.549 kb on + strand, within DvMF_2031 at 2516.598 kb on + strand, within DvMF_2031 at 2516.802 kb on - strand, within DvMF_2031 at 2516.809 kb on + strand, within DvMF_2031 at 2517.049 kb on - strand, within DvMF_2031 at 2517.160 kb on - strand, within DvMF_2031 at 2517.432 kb on + strand, within DvMF_2031 at 2517.587 kb on - strand, within DvMF_2031 at 2517.664 kb on - strand, within DvMF_2031 at 2517.691 kb on - strand, within DvMF_2031 at 2517.720 kb on + strand, within DvMF_2031 at 2517.748 kb on + strand, within DvMF_2031 at 2517.783 kb on + strand, within DvMF_2031 at 2517.990 kb on - strand, within DvMF_2031 at 2518.212 kb on + strand at 2518.299 kb on - strand at 2518.312 kb on - strand at 2518.321 kb on + strand at 2518.383 kb on + strand at 2518.391 kb on - strand at 2518.391 kb on - strand at 2518.391 kb on - strand at 2518.391 kb on - strand at 2518.486 kb on - strand at 2518.510 kb on + strand at 2518.512 kb on - strand at 2518.667 kb on + strand, within DvMF_2032 at 2518.686 kb on - strand, within DvMF_2032 at 2518.854 kb on - strand, within DvMF_2032 at 2519.077 kb on - strand, within DvMF_2032 at 2519.077 kb on - strand, within DvMF_2032 at 2519.139 kb on - strand, within DvMF_2032 at 2519.221 kb on + strand, within DvMF_2032
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.0125 mg/ml remove 2,515,197 + DvMF_2030 0.60 -0.3 2,515,212 - DvMF_2030 0.62 -0.5 2,515,432 + -0.5 2,515,595 + -0.1 2,515,901 + +0.6 2,516,004 - -0.3 2,516,130 - -0.7 2,516,140 - -0.4 2,516,226 + -0.1 2,516,234 - -0.7 2,516,296 + -0.6 2,516,351 + DvMF_2031 0.12 +0.2 2,516,359 - DvMF_2031 0.12 -0.6 2,516,397 - DvMF_2031 0.14 -0.6 2,516,399 + DvMF_2031 0.14 -0.3 2,516,482 + DvMF_2031 0.18 -0.4 2,516,519 - DvMF_2031 0.19 -0.2 2,516,543 - DvMF_2031 0.20 -0.3 2,516,549 + DvMF_2031 0.21 -0.0 2,516,598 + DvMF_2031 0.23 -0.4 2,516,802 - DvMF_2031 0.32 -0.2 2,516,809 + DvMF_2031 0.32 -0.9 2,517,049 - DvMF_2031 0.42 -0.8 2,517,160 - DvMF_2031 0.47 -0.1 2,517,432 + DvMF_2031 0.59 -0.4 2,517,587 - DvMF_2031 0.66 -0.7 2,517,664 - DvMF_2031 0.69 -1.2 2,517,691 - DvMF_2031 0.70 -0.4 2,517,720 + DvMF_2031 0.71 -0.8 2,517,748 + DvMF_2031 0.73 -0.3 2,517,783 + DvMF_2031 0.74 -0.5 2,517,990 - DvMF_2031 0.83 -0.4 2,518,212 + -0.5 2,518,299 - -1.2 2,518,312 - -1.1 2,518,321 + -0.4 2,518,383 + -0.3 2,518,391 - -0.0 2,518,391 - -0.1 2,518,391 - +0.0 2,518,391 - -0.4 2,518,486 - -0.3 2,518,510 + +0.2 2,518,512 - -0.1 2,518,667 + DvMF_2032 0.17 -0.4 2,518,686 - DvMF_2032 0.19 -0.4 2,518,854 - DvMF_2032 0.39 -0.3 2,519,077 - DvMF_2032 0.65 -0.2 2,519,077 - DvMF_2032 0.65 -0.0 2,519,139 - DvMF_2032 0.73 -0.8 2,519,221 + DvMF_2032 0.82 -0.2
Or see this region's nucleotide sequence