Experiment: MoYLS4 with Spectinomycin 0.0125 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0198 and DvMF_0199 are separated by 114 nucleotides DvMF_0199 and DvMF_0200 overlap by 1 nucleotides DvMF_0200 and DvMF_0201 are separated by 189 nucleotides DvMF_0201 and DvMF_0202 are separated by 88 nucleotides
DvMF_0198: DvMF_0198 - hypothetical protein (RefSeq), at 217,001 to 217,324
_0198
DvMF_0199: DvMF_0199 - ATP:corrinoid adenosyltransferase BtuR/CobO/CobP (RefSeq), at 217,439 to 217,942
_0199
DvMF_0200: DvMF_0200 - Radical SAM domain protein (RefSeq), at 217,942 to 219,102
_0200
DvMF_0201: DvMF_0201 - hypothetical protein (RefSeq), at 219,292 to 219,768
_0201
DvMF_0202: DvMF_0202 - phosphoribosylaminoimidazole synthetase (RefSeq), at 219,857 to 220,909
_0202
Position (kb) 217
218
219
220 Strain fitness (log2 ratio) -2
-1
0
1 at 216.967 kb on - strand at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.271 kb on + strand, within DvMF_0198 at 217.279 kb on - strand, within DvMF_0198 at 217.279 kb on - strand, within DvMF_0198 at 217.429 kb on + strand at 217.653 kb on - strand, within DvMF_0199 at 217.716 kb on + strand, within DvMF_0199 at 217.724 kb on - strand, within DvMF_0199 at 218.308 kb on - strand, within DvMF_0200 at 218.377 kb on - strand, within DvMF_0200 at 218.396 kb on + strand, within DvMF_0200 at 218.764 kb on - strand, within DvMF_0200 at 218.847 kb on - strand, within DvMF_0200 at 218.852 kb on + strand, within DvMF_0200 at 218.852 kb on + strand, within DvMF_0200 at 218.860 kb on - strand, within DvMF_0200 at 218.995 kb on - strand at 219.184 kb on + strand at 219.325 kb on + strand at 219.333 kb on - strand at 219.886 kb on - strand at 219.958 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.0125 mg/ml remove 216,967 - -1.1 217,271 + DvMF_0198 0.83 -0.5 217,271 + DvMF_0198 0.83 -0.9 217,271 + DvMF_0198 0.83 -0.1 217,271 + DvMF_0198 0.83 -0.4 217,271 + DvMF_0198 0.83 -0.4 217,279 - DvMF_0198 0.86 -0.4 217,279 - DvMF_0198 0.86 -1.0 217,429 + -0.2 217,653 - DvMF_0199 0.42 -2.2 217,716 + DvMF_0199 0.55 -2.2 217,724 - DvMF_0199 0.57 -2.3 218,308 - DvMF_0200 0.32 -0.4 218,377 - DvMF_0200 0.37 -0.2 218,396 + DvMF_0200 0.39 -0.3 218,764 - DvMF_0200 0.71 +0.1 218,847 - DvMF_0200 0.78 -0.1 218,852 + DvMF_0200 0.78 -0.3 218,852 + DvMF_0200 0.78 -0.2 218,860 - DvMF_0200 0.79 +0.2 218,995 - +0.1 219,184 + -0.6 219,325 + -0.0 219,333 - -0.6 219,886 - -0.7 219,958 + -0.7
Or see this region's nucleotide sequence