Experiment: defined media with 30 mM sodium lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1512 and Dsui_1513 are separated by 163 nucleotides Dsui_1513 and Dsui_1514 overlap by 4 nucleotides Dsui_1514 and Dsui_1515 are separated by 15 nucleotides
Dsui_1512: Dsui_1512 - Na+/H+ antiporter NhaD-like permease, at 1,576,011 to 1,577,246
_1512
Dsui_1513: Dsui_1513 - hypothetical protein, at 1,577,410 to 1,577,547
_1513
Dsui_1514: Dsui_1514 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase, at 1,577,544 to 1,578,017
_1514
Dsui_1515: Dsui_1515 - poly(A) polymerase, at 1,578,033 to 1,579,418
_1515
Position (kb)
1577
1578 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1576.415 kb on - strand, within Dsui_1512 at 1576.424 kb on + strand, within Dsui_1512 at 1576.432 kb on - strand, within Dsui_1512 at 1576.437 kb on + strand, within Dsui_1512 at 1576.437 kb on + strand, within Dsui_1512 at 1576.438 kb on - strand, within Dsui_1512 at 1576.475 kb on + strand, within Dsui_1512 at 1576.475 kb on - strand, within Dsui_1512 at 1576.508 kb on + strand, within Dsui_1512 at 1576.516 kb on - strand, within Dsui_1512 at 1576.516 kb on - strand at 1576.516 kb on - strand, within Dsui_1512 at 1576.557 kb on + strand, within Dsui_1512 at 1576.558 kb on - strand, within Dsui_1512 at 1576.649 kb on - strand, within Dsui_1512 at 1576.664 kb on + strand, within Dsui_1512 at 1576.688 kb on + strand, within Dsui_1512 at 1576.694 kb on + strand, within Dsui_1512 at 1576.695 kb on + strand, within Dsui_1512 at 1576.696 kb on - strand, within Dsui_1512 at 1576.702 kb on - strand, within Dsui_1512 at 1576.709 kb on + strand, within Dsui_1512 at 1576.709 kb on - strand, within Dsui_1512 at 1576.739 kb on + strand, within Dsui_1512 at 1576.752 kb on + strand at 1576.760 kb on - strand, within Dsui_1512 at 1576.831 kb on + strand, within Dsui_1512 at 1576.831 kb on + strand, within Dsui_1512 at 1576.841 kb on + strand, within Dsui_1512 at 1576.917 kb on + strand, within Dsui_1512 at 1576.922 kb on - strand, within Dsui_1512 at 1576.954 kb on + strand, within Dsui_1512 at 1576.959 kb on + strand, within Dsui_1512 at 1576.963 kb on + strand, within Dsui_1512 at 1577.005 kb on + strand at 1577.013 kb on - strand, within Dsui_1512 at 1577.020 kb on - strand, within Dsui_1512 at 1577.026 kb on + strand, within Dsui_1512 at 1577.034 kb on - strand, within Dsui_1512 at 1577.034 kb on - strand, within Dsui_1512 at 1577.056 kb on - strand, within Dsui_1512 at 1577.064 kb on - strand, within Dsui_1512 at 1577.074 kb on - strand, within Dsui_1512 at 1577.074 kb on - strand, within Dsui_1512 at 1577.074 kb on - strand, within Dsui_1512 at 1577.091 kb on + strand at 1577.135 kb on + strand at 1577.140 kb on + strand at 1577.150 kb on + strand at 1577.150 kb on + strand at 1577.150 kb on + strand at 1577.158 kb on - strand at 1577.158 kb on - strand at 1577.158 kb on - strand at 1577.158 kb on - strand at 1577.158 kb on - strand at 1577.158 kb on - strand at 1577.263 kb on + strand at 1577.263 kb on + strand at 1577.263 kb on + strand at 1577.270 kb on + strand at 1577.270 kb on + strand at 1577.271 kb on - strand at 1577.271 kb on - strand at 1577.278 kb on - strand at 1577.305 kb on - strand at 1577.305 kb on - strand at 1577.324 kb on + strand at 1577.340 kb on - strand at 1577.342 kb on + strand at 1577.406 kb on - strand at 1577.433 kb on + strand at 1577.455 kb on - strand, within Dsui_1513 at 1577.459 kb on + strand, within Dsui_1513 at 1577.465 kb on - strand, within Dsui_1513 at 1577.474 kb on - strand, within Dsui_1513 at 1577.492 kb on - strand, within Dsui_1513 at 1577.502 kb on + strand, within Dsui_1513 at 1577.534 kb on + strand at 1578.136 kb on + strand at 1578.268 kb on + strand, within Dsui_1515
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 30 mM sodium lactate remove 1,576,415 - Dsui_1512 0.33 -0.4 1,576,424 + Dsui_1512 0.33 +1.3 1,576,432 - Dsui_1512 0.34 +0.0 1,576,437 + Dsui_1512 0.34 -0.1 1,576,437 + Dsui_1512 0.34 -0.3 1,576,438 - Dsui_1512 0.35 +0.9 1,576,475 + Dsui_1512 0.38 -0.4 1,576,475 - Dsui_1512 0.38 +0.2 1,576,508 + Dsui_1512 0.40 +0.3 1,576,516 - Dsui_1512 0.41 +0.1 1,576,516 - +1.5 1,576,516 - Dsui_1512 0.41 +0.1 1,576,557 + Dsui_1512 0.44 +0.7 1,576,558 - Dsui_1512 0.44 +0.2 1,576,649 - Dsui_1512 0.52 -0.4 1,576,664 + Dsui_1512 0.53 -0.4 1,576,688 + Dsui_1512 0.55 -0.7 1,576,694 + Dsui_1512 0.55 -0.7 1,576,695 + Dsui_1512 0.55 +0.5 1,576,696 - Dsui_1512 0.55 -0.3 1,576,702 - Dsui_1512 0.56 -1.0 1,576,709 + Dsui_1512 0.56 -0.2 1,576,709 - Dsui_1512 0.56 -0.1 1,576,739 + Dsui_1512 0.59 +0.9 1,576,752 + +0.5 1,576,760 - Dsui_1512 0.61 -0.5 1,576,831 + Dsui_1512 0.66 -1.0 1,576,831 + Dsui_1512 0.66 -1.6 1,576,841 + Dsui_1512 0.67 +0.5 1,576,917 + Dsui_1512 0.73 -0.2 1,576,922 - Dsui_1512 0.74 -0.3 1,576,954 + Dsui_1512 0.76 -1.0 1,576,959 + Dsui_1512 0.77 -1.7 1,576,963 + Dsui_1512 0.77 -0.3 1,577,005 + +1.5 1,577,013 - Dsui_1512 0.81 +0.2 1,577,020 - Dsui_1512 0.82 +0.2 1,577,026 + Dsui_1512 0.82 +0.2 1,577,034 - Dsui_1512 0.83 +0.5 1,577,034 - Dsui_1512 0.83 +0.3 1,577,056 - Dsui_1512 0.85 -0.2 1,577,064 - Dsui_1512 0.85 -0.4 1,577,074 - Dsui_1512 0.86 +0.4 1,577,074 - Dsui_1512 0.86 -0.0 1,577,074 - Dsui_1512 0.86 -0.1 1,577,091 + +1.3 1,577,135 + -0.9 1,577,140 + +0.8 1,577,150 + -0.2 1,577,150 + -1.0 1,577,150 + -0.4 1,577,158 - -1.0 1,577,158 - -0.4 1,577,158 - +1.6 1,577,158 - +0.1 1,577,158 - -0.3 1,577,158 - -0.4 1,577,263 + +1.1 1,577,263 + +0.4 1,577,263 + +0.3 1,577,270 + -0.3 1,577,270 + +0.4 1,577,271 - +0.0 1,577,271 - +0.4 1,577,278 - -0.3 1,577,305 - +0.3 1,577,305 - +0.4 1,577,324 + -0.3 1,577,340 - -0.7 1,577,342 + +0.9 1,577,406 - -0.1 1,577,433 + +2.0 1,577,455 - Dsui_1513 0.33 -0.0 1,577,459 + Dsui_1513 0.36 -0.7 1,577,465 - Dsui_1513 0.40 -0.2 1,577,474 - Dsui_1513 0.46 -0.1 1,577,492 - Dsui_1513 0.59 +0.3 1,577,502 + Dsui_1513 0.67 -0.1 1,577,534 + +0.3 1,578,136 + -1.8 1,578,268 + Dsui_1515 0.17 -1.1
Or see this region's nucleotide sequence