Experiment: MoYLS4 with G418 0.031 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0413 and DvMF_0414 are separated by 75 nucleotides DvMF_0414 and DvMF_0415 are separated by 281 nucleotides DvMF_0415 and DvMF_0416 are separated by 252 nucleotides
DvMF_0413: DvMF_0413 - putative signal transduction protein with CBS domains (RefSeq), at 482,452 to 482,880
_0413
DvMF_0414: DvMF_0414 - Peptidase M23 (RefSeq), at 482,956 to 484,266
_0414
DvMF_0415: DvMF_0415 - protein of unknown function DUF488 (RefSeq), at 484,548 to 484,940
_0415
DvMF_0416: DvMF_0416 - pyruvate phosphate dikinase PEP/pyruvate-binding (RefSeq), at 485,193 to 488,771
_0416
Position (kb) 482
483
484
485 Strain fitness (log2 ratio) -2
-1
0
1 at 482.085 kb on - strand at 482.122 kb on - strand at 482.669 kb on - strand, within DvMF_0413 at 482.797 kb on + strand, within DvMF_0413 at 482.864 kb on - strand at 482.874 kb on + strand at 482.904 kb on - strand at 483.050 kb on - strand at 483.339 kb on + strand, within DvMF_0414 at 483.339 kb on + strand, within DvMF_0414 at 483.339 kb on + strand, within DvMF_0414 at 483.347 kb on - strand, within DvMF_0414 at 483.478 kb on + strand, within DvMF_0414 at 483.905 kb on + strand, within DvMF_0414 at 484.315 kb on + strand at 484.425 kb on - strand at 484.545 kb on + strand at 484.553 kb on - strand at 484.685 kb on + strand, within DvMF_0415 at 485.232 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with G418 0.031 mg/ml remove 482,085 - -0.6 482,122 - -0.0 482,669 - DvMF_0413 0.51 -2.0 482,797 + DvMF_0413 0.80 -1.8 482,864 - -1.0 482,874 + -0.3 482,904 - -0.0 483,050 - -1.1 483,339 + DvMF_0414 0.29 -1.0 483,339 + DvMF_0414 0.29 -1.2 483,339 + DvMF_0414 0.29 -1.5 483,347 - DvMF_0414 0.30 -1.3 483,478 + DvMF_0414 0.40 -2.5 483,905 + DvMF_0414 0.72 -1.2 484,315 + -0.1 484,425 - +0.2 484,545 + -0.5 484,553 - -0.3 484,685 + DvMF_0415 0.35 -0.4 485,232 - -0.2
Or see this region's nucleotide sequence