Experiment: m.b. perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3311 and Dshi_3312 are separated by 146 nucleotides Dshi_3312 and Dshi_3313 overlap by 4 nucleotides Dshi_3313 and Dshi_3314 are separated by 445 nucleotides
Dshi_3311: Dshi_3311 - radical SAM enzyme, Cfr family (RefSeq), at 3,474,155 to 3,475,375
_3311
Dshi_3312: Dshi_3312 - hypothetical protein (RefSeq), at 3,475,522 to 3,476,199
_3312
Dshi_3313: Dshi_3313 - hypothetical protein (RefSeq), at 3,476,196 to 3,476,906
_3313
Dshi_3314: Dshi_3314 - hypothetical protein (RefSeq), at 3,477,352 to 3,477,885
_3314
Position (kb)
3476
3477 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3475.245 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand, within Dshi_3311 at 3475.250 kb on + strand at 3475.258 kb on - strand at 3475.258 kb on - strand at 3475.282 kb on - strand at 3475.290 kb on - strand at 3475.301 kb on + strand at 3475.305 kb on + strand at 3475.345 kb on - strand at 3475.349 kb on + strand at 3475.373 kb on - strand at 3475.373 kb on - strand at 3475.494 kb on - strand at 3475.496 kb on + strand at 3475.524 kb on + strand at 3475.524 kb on + strand at 3475.532 kb on - strand at 3475.532 kb on - strand at 3475.537 kb on + strand at 3475.545 kb on - strand at 3475.545 kb on - strand at 3475.622 kb on - strand, within Dshi_3312 at 3475.652 kb on + strand, within Dshi_3312 at 3475.660 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.699 kb on - strand, within Dshi_3312 at 3475.704 kb on + strand, within Dshi_3312 at 3475.734 kb on - strand, within Dshi_3312 at 3475.783 kb on - strand, within Dshi_3312 at 3475.838 kb on + strand, within Dshi_3312 at 3476.194 kb on + strand at 3476.245 kb on + strand at 3476.269 kb on - strand, within Dshi_3313 at 3476.277 kb on + strand, within Dshi_3313 at 3476.285 kb on - strand, within Dshi_3313 at 3476.319 kb on - strand, within Dshi_3313 at 3476.338 kb on - strand, within Dshi_3313 at 3476.481 kb on + strand, within Dshi_3313 at 3476.483 kb on - strand, within Dshi_3313 at 3476.489 kb on - strand, within Dshi_3313 at 3476.489 kb on - strand, within Dshi_3313 at 3476.489 kb on - strand, within Dshi_3313 at 3476.493 kb on + strand, within Dshi_3313 at 3476.591 kb on - strand, within Dshi_3313 at 3476.649 kb on - strand, within Dshi_3313 at 3476.781 kb on + strand, within Dshi_3313 at 3476.794 kb on - strand, within Dshi_3313 at 3476.941 kb on + strand at 3477.090 kb on + strand at 3477.098 kb on - strand at 3477.109 kb on + strand at 3477.109 kb on + strand at 3477.135 kb on - strand at 3477.166 kb on + strand at 3477.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. perchlorate 50 mM remove 3,475,245 + Dshi_3311 0.89 -0.4 3,475,250 + Dshi_3311 0.90 -2.0 3,475,250 + +0.4 3,475,258 - -0.2 3,475,258 - +2.0 3,475,282 - -1.7 3,475,290 - -1.3 3,475,301 + -2.4 3,475,305 + +0.4 3,475,345 - -1.0 3,475,349 + -0.6 3,475,373 - +0.4 3,475,373 - -1.2 3,475,494 - -1.2 3,475,496 + -3.4 3,475,524 + +0.8 3,475,524 + -2.6 3,475,532 - -1.2 3,475,532 - -1.7 3,475,537 + -3.6 3,475,545 - -1.0 3,475,545 - +0.4 3,475,622 - Dshi_3312 0.15 -1.3 3,475,652 + Dshi_3312 0.19 -0.0 3,475,660 - Dshi_3312 0.20 -1.0 3,475,699 - Dshi_3312 0.26 -2.8 3,475,699 - Dshi_3312 0.26 +1.4 3,475,704 + Dshi_3312 0.27 +2.0 3,475,734 - Dshi_3312 0.31 +2.4 3,475,783 - Dshi_3312 0.38 +1.0 3,475,838 + Dshi_3312 0.47 +0.4 3,476,194 + +1.0 3,476,245 + -2.4 3,476,269 - Dshi_3313 0.10 -2.0 3,476,277 + Dshi_3313 0.11 +1.4 3,476,285 - Dshi_3313 0.13 +0.4 3,476,319 - Dshi_3313 0.17 -0.2 3,476,338 - Dshi_3313 0.20 +0.4 3,476,481 + Dshi_3313 0.40 -1.2 3,476,483 - Dshi_3313 0.40 +0.4 3,476,489 - Dshi_3313 0.41 -1.6 3,476,489 - Dshi_3313 0.41 +0.8 3,476,489 - Dshi_3313 0.41 -3.8 3,476,493 + Dshi_3313 0.42 +0.4 3,476,591 - Dshi_3313 0.56 -1.0 3,476,649 - Dshi_3313 0.64 -1.6 3,476,781 + Dshi_3313 0.82 -2.2 3,476,794 - Dshi_3313 0.84 -0.6 3,476,941 + -0.7 3,477,090 + +0.9 3,477,098 - +0.4 3,477,109 + +3.2 3,477,109 + -0.1 3,477,135 - -0.2 3,477,166 + +0.2 3,477,248 - -0.4
Or see this region's nucleotide sequence