Experiment: m.b. perchlorate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2221 and Dshi_2222 are separated by 38 nucleotides Dshi_2222 and Dshi_2223 are separated by 74 nucleotides Dshi_2223 and Dshi_2224 overlap by 4 nucleotides Dshi_2224 and Dshi_2225 are separated by 68 nucleotides
Dshi_2221: Dshi_2221 - ABC transporter related (RefSeq), at 2,344,582 to 2,345,361
_2221
Dshi_2222: Dshi_2222 - extracellular solute-binding protein family 3 (RefSeq), at 2,345,400 to 2,346,128
_2222
Dshi_2223: Dshi_2223 - binding-protein-dependent transport systems inner membrane component (RefSeq), at 2,346,203 to 2,347,090
_2223
Dshi_2224: Dshi_2224 - polar amino acid ABC transporter, inner membrane subunit (RefSeq), at 2,347,087 to 2,347,893
_2224
Dshi_2225: Dshi_2225 - glutamine amidotransferase class-I (RefSeq), at 2,347,962 to 2,348,672
_2225
Position (kb)
2346
2347
2348 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2345.340 kb on + strand at 2345.407 kb on - strand at 2345.414 kb on + strand at 2345.424 kb on + strand at 2345.434 kb on + strand at 2345.435 kb on + strand at 2345.536 kb on + strand, within Dshi_2222 at 2345.642 kb on - strand, within Dshi_2222 at 2345.680 kb on + strand, within Dshi_2222 at 2345.717 kb on + strand, within Dshi_2222 at 2345.737 kb on - strand, within Dshi_2222 at 2345.742 kb on + strand, within Dshi_2222 at 2345.779 kb on - strand, within Dshi_2222 at 2345.858 kb on - strand, within Dshi_2222 at 2345.965 kb on - strand, within Dshi_2222 at 2345.965 kb on - strand, within Dshi_2222 at 2346.117 kb on + strand at 2346.122 kb on + strand at 2346.225 kb on + strand at 2346.314 kb on - strand, within Dshi_2223 at 2346.324 kb on - strand, within Dshi_2223 at 2346.336 kb on - strand, within Dshi_2223 at 2346.346 kb on + strand, within Dshi_2223 at 2346.379 kb on + strand, within Dshi_2223 at 2346.379 kb on + strand, within Dshi_2223 at 2346.387 kb on - strand, within Dshi_2223 at 2346.428 kb on - strand, within Dshi_2223 at 2346.435 kb on - strand, within Dshi_2223 at 2346.469 kb on - strand, within Dshi_2223 at 2346.597 kb on + strand, within Dshi_2223 at 2346.613 kb on - strand, within Dshi_2223 at 2346.704 kb on - strand, within Dshi_2223 at 2346.717 kb on - strand, within Dshi_2223 at 2346.789 kb on - strand, within Dshi_2223 at 2346.807 kb on + strand, within Dshi_2223 at 2346.813 kb on + strand, within Dshi_2223 at 2346.813 kb on + strand, within Dshi_2223 at 2346.835 kb on + strand, within Dshi_2223 at 2347.039 kb on + strand at 2347.048 kb on - strand at 2347.077 kb on - strand at 2347.113 kb on - strand at 2347.196 kb on + strand, within Dshi_2224 at 2347.214 kb on - strand, within Dshi_2224 at 2347.264 kb on + strand, within Dshi_2224 at 2347.287 kb on + strand, within Dshi_2224 at 2347.290 kb on - strand, within Dshi_2224 at 2347.290 kb on - strand, within Dshi_2224 at 2347.373 kb on + strand, within Dshi_2224 at 2347.428 kb on + strand, within Dshi_2224 at 2347.466 kb on + strand, within Dshi_2224 at 2347.512 kb on + strand, within Dshi_2224 at 2347.697 kb on - strand, within Dshi_2224 at 2347.707 kb on - strand, within Dshi_2224 at 2347.712 kb on - strand, within Dshi_2224 at 2347.716 kb on + strand, within Dshi_2224 at 2347.716 kb on + strand, within Dshi_2224 at 2347.756 kb on - strand, within Dshi_2224 at 2347.757 kb on - strand, within Dshi_2224 at 2347.846 kb on + strand at 2347.887 kb on - strand at 2347.892 kb on - strand at 2347.985 kb on - strand at 2348.062 kb on + strand, within Dshi_2225
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. perchlorate 50 mM remove 2,345,340 + -1.1 2,345,407 - +1.0 2,345,414 + -1.1 2,345,424 + -0.0 2,345,434 + +0.5 2,345,435 + +1.3 2,345,536 + Dshi_2222 0.19 +0.1 2,345,642 - Dshi_2222 0.33 -0.1 2,345,680 + Dshi_2222 0.38 -0.8 2,345,717 + Dshi_2222 0.43 +1.0 2,345,737 - Dshi_2222 0.46 -2.1 2,345,742 + Dshi_2222 0.47 -0.9 2,345,779 - Dshi_2222 0.52 -0.6 2,345,858 - Dshi_2222 0.63 -2.0 2,345,965 - Dshi_2222 0.78 -2.1 2,345,965 - Dshi_2222 0.78 -1.1 2,346,117 + +1.6 2,346,122 + -1.5 2,346,225 + -1.4 2,346,314 - Dshi_2223 0.12 -0.8 2,346,324 - Dshi_2223 0.14 +0.0 2,346,336 - Dshi_2223 0.15 +0.4 2,346,346 + Dshi_2223 0.16 +3.0 2,346,379 + Dshi_2223 0.20 -0.1 2,346,379 + Dshi_2223 0.20 -0.2 2,346,387 - Dshi_2223 0.21 +3.0 2,346,428 - Dshi_2223 0.25 +0.3 2,346,435 - Dshi_2223 0.26 +1.2 2,346,469 - Dshi_2223 0.30 -0.6 2,346,597 + Dshi_2223 0.44 +0.1 2,346,613 - Dshi_2223 0.46 +0.7 2,346,704 - Dshi_2223 0.56 +1.0 2,346,717 - Dshi_2223 0.58 -2.3 2,346,789 - Dshi_2223 0.66 +1.0 2,346,807 + Dshi_2223 0.68 +0.2 2,346,813 + Dshi_2223 0.69 -2.1 2,346,813 + Dshi_2223 0.69 +0.7 2,346,835 + Dshi_2223 0.71 -1.4 2,347,039 + -0.3 2,347,048 - -0.8 2,347,077 - +2.2 2,347,113 - +0.7 2,347,196 + Dshi_2224 0.14 -1.3 2,347,214 - Dshi_2224 0.16 -0.8 2,347,264 + Dshi_2224 0.22 -1.3 2,347,287 + Dshi_2224 0.25 +0.6 2,347,290 - Dshi_2224 0.25 +2.8 2,347,290 - Dshi_2224 0.25 +0.4 2,347,373 + Dshi_2224 0.35 +0.2 2,347,428 + Dshi_2224 0.42 +0.6 2,347,466 + Dshi_2224 0.47 +1.8 2,347,512 + Dshi_2224 0.53 -2.7 2,347,697 - Dshi_2224 0.76 +3.7 2,347,707 - Dshi_2224 0.77 +1.8 2,347,712 - Dshi_2224 0.77 +0.4 2,347,716 + Dshi_2224 0.78 +0.8 2,347,716 + Dshi_2224 0.78 -0.8 2,347,756 - Dshi_2224 0.83 +0.5 2,347,757 - Dshi_2224 0.83 -0.2 2,347,846 + -0.6 2,347,887 - -2.1 2,347,892 - +0.7 2,347,985 - -2.2 2,348,062 + Dshi_2225 0.14 -1.6
Or see this region's nucleotide sequence