Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_4115 and HP15_4116 are separated by 106 nucleotides HP15_4116 and HP15_4117 are separated by 97 nucleotides HP15_4117 and HP15_4118 overlap by 4 nucleotides HP15_4118 and HP15_4119 are separated by 216 nucleotides HP15_4119 and HP15_4120 are separated by 141 nucleotides
HP15_4115: HP15_4115 - sensory box/GGDEF/EAL family protein, at 4,349,658 to 4,352,093
_4115
HP15_4116: HP15_4116 - SirA-like protein, at 4,352,200 to 4,352,334
_4116
HP15_4117: HP15_4117 - membrane or secreted protein, at 4,352,432 to 4,352,560
_4117
HP15_4118: HP15_4118 - formate dehydrogenase, gamma subunit, at 4,352,557 to 4,353,600
_4118
HP15_4119: HP15_4119 - 4Fe-4S ferredoxin, iron-sulfur binding domain protein, at 4,353,817 to 4,354,299
_4119
HP15_4120: HP15_4120 - secreted protein containing molybdopterin oxidoreductase / molybdopterin dinucleotide-binding region, at 4,354,441 to 4,357,326
_4120
Position (kb)
4352
4353
4354 Strain fitness (log2 ratio)
-1
0
1
2 at 4351.605 kb on - strand, within HP15_4115 at 4351.605 kb on - strand, within HP15_4115 at 4351.668 kb on + strand, within HP15_4115 at 4351.676 kb on - strand, within HP15_4115 at 4351.676 kb on - strand, within HP15_4115 at 4351.748 kb on + strand, within HP15_4115 at 4351.756 kb on - strand, within HP15_4115 at 4351.943 kb on + strand at 4351.943 kb on + strand at 4351.948 kb on + strand at 4351.951 kb on - strand at 4351.951 kb on - strand at 4351.951 kb on - strand at 4351.984 kb on - strand at 4352.014 kb on + strand at 4352.042 kb on + strand at 4352.089 kb on - strand at 4352.163 kb on + strand at 4352.224 kb on + strand, within HP15_4116 at 4352.295 kb on + strand, within HP15_4116 at 4352.344 kb on + strand at 4352.395 kb on - strand at 4352.395 kb on - strand at 4352.395 kb on - strand at 4352.420 kb on + strand at 4352.420 kb on + strand at 4352.420 kb on + strand at 4352.428 kb on - strand at 4352.433 kb on - strand at 4352.457 kb on + strand, within HP15_4117 at 4352.465 kb on - strand, within HP15_4117 at 4352.684 kb on - strand, within HP15_4118 at 4352.713 kb on - strand, within HP15_4118 at 4352.738 kb on - strand, within HP15_4118 at 4352.857 kb on - strand, within HP15_4118 at 4352.971 kb on + strand, within HP15_4118 at 4353.019 kb on + strand, within HP15_4118 at 4353.051 kb on - strand, within HP15_4118 at 4353.185 kb on - strand, within HP15_4118 at 4353.236 kb on + strand, within HP15_4118 at 4353.244 kb on - strand, within HP15_4118 at 4353.254 kb on + strand, within HP15_4118 at 4353.375 kb on + strand, within HP15_4118 at 4353.383 kb on - strand, within HP15_4118 at 4353.393 kb on - strand, within HP15_4118 at 4353.412 kb on - strand, within HP15_4118 at 4353.604 kb on + strand at 4353.614 kb on - strand at 4353.621 kb on - strand at 4353.644 kb on + strand at 4353.671 kb on + strand at 4353.671 kb on + strand at 4353.671 kb on + strand at 4353.679 kb on - strand at 4353.813 kb on - strand at 4353.847 kb on - strand at 4353.934 kb on + strand, within HP15_4119 at 4354.006 kb on + strand, within HP15_4119 at 4354.124 kb on + strand, within HP15_4119 at 4354.170 kb on + strand, within HP15_4119 at 4354.170 kb on + strand, within HP15_4119 at 4354.170 kb on + strand, within HP15_4119 at 4354.170 kb on + strand, within HP15_4119 at 4354.170 kb on + strand, within HP15_4119 at 4354.180 kb on + strand, within HP15_4119 at 4354.215 kb on + strand, within HP15_4119 at 4354.217 kb on + strand, within HP15_4119 at 4354.217 kb on + strand, within HP15_4119 at 4354.238 kb on - strand, within HP15_4119 at 4354.238 kb on - strand, within HP15_4119 at 4354.284 kb on + strand at 4354.284 kb on + strand at 4354.343 kb on - strand at 4354.426 kb on + strand at 4354.426 kb on + strand at 4354.426 kb on + strand at 4354.469 kb on + strand at 4354.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 4,351,605 - HP15_4115 0.80 +0.0 4,351,605 - HP15_4115 0.80 +0.9 4,351,668 + HP15_4115 0.83 +0.6 4,351,676 - HP15_4115 0.83 -0.6 4,351,676 - HP15_4115 0.83 -0.9 4,351,748 + HP15_4115 0.86 -0.4 4,351,756 - HP15_4115 0.86 -0.4 4,351,943 + -0.1 4,351,943 + -0.1 4,351,948 + +0.9 4,351,951 - +0.0 4,351,951 - -0.2 4,351,951 - -0.7 4,351,984 - +1.9 4,352,014 + -0.2 4,352,042 + +1.1 4,352,089 - -0.2 4,352,163 + +0.8 4,352,224 + HP15_4116 0.18 -1.4 4,352,295 + HP15_4116 0.70 +0.1 4,352,344 + -0.6 4,352,395 - +0.1 4,352,395 - -1.0 4,352,395 - -0.3 4,352,420 + +0.6 4,352,420 + +0.0 4,352,420 + +1.3 4,352,428 - -0.3 4,352,433 - +0.5 4,352,457 + HP15_4117 0.19 -0.0 4,352,465 - HP15_4117 0.26 +1.4 4,352,684 - HP15_4118 0.12 +0.2 4,352,713 - HP15_4118 0.15 +0.1 4,352,738 - HP15_4118 0.17 +0.6 4,352,857 - HP15_4118 0.29 +0.9 4,352,971 + HP15_4118 0.40 +1.3 4,353,019 + HP15_4118 0.44 +0.1 4,353,051 - HP15_4118 0.47 +0.7 4,353,185 - HP15_4118 0.60 +0.8 4,353,236 + HP15_4118 0.65 +0.6 4,353,244 - HP15_4118 0.66 +0.2 4,353,254 + HP15_4118 0.67 +1.1 4,353,375 + HP15_4118 0.78 +0.6 4,353,383 - HP15_4118 0.79 +0.3 4,353,393 - HP15_4118 0.80 -0.7 4,353,412 - HP15_4118 0.82 +0.7 4,353,604 + +0.5 4,353,614 - +0.1 4,353,621 - +1.0 4,353,644 + -0.0 4,353,671 + +0.2 4,353,671 + +0.4 4,353,671 + +0.0 4,353,679 - +1.3 4,353,813 - -0.4 4,353,847 - -0.7 4,353,934 + HP15_4119 0.24 +0.7 4,354,006 + HP15_4119 0.39 +0.0 4,354,124 + HP15_4119 0.64 +1.2 4,354,170 + HP15_4119 0.73 -0.4 4,354,170 + HP15_4119 0.73 +0.2 4,354,170 + HP15_4119 0.73 -0.7 4,354,170 + HP15_4119 0.73 +0.1 4,354,170 + HP15_4119 0.73 +1.1 4,354,180 + HP15_4119 0.75 -0.4 4,354,215 + HP15_4119 0.82 +0.1 4,354,217 + HP15_4119 0.83 -0.4 4,354,217 + HP15_4119 0.83 -0.6 4,354,238 - HP15_4119 0.87 +0.3 4,354,238 - HP15_4119 0.87 -0.1 4,354,284 + -0.5 4,354,284 + -1.6 4,354,343 - +0.1 4,354,426 + -0.7 4,354,426 + +0.5 4,354,426 + +1.3 4,354,469 + +1.4 4,354,547 - +1.3
Or see this region's nucleotide sequence