Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3323 and HP15_3324 are separated by 64 nucleotides HP15_3324 and HP15_3325 are separated by 65 nucleotides HP15_3325 and HP15_3326 are separated by 143 nucleotides HP15_3326 and HP15_3327 are separated by 176 nucleotides
HP15_3323: HP15_3323 - ribosomal protein L33, at 3,484,414 to 3,484,569
_3323
HP15_3324: HP15_3324 - endonuclease/exonuclease/phosphatase, at 3,484,634 to 3,485,494
_3324
HP15_3325: HP15_3325 - DSBA oxidoreductase, at 3,485,560 to 3,486,195
_3325
HP15_3326: HP15_3326 - cytochrome c, class I, at 3,486,339 to 3,486,947
_3326
HP15_3327: HP15_3327 - GTP-binding protein EngB, at 3,487,124 to 3,487,771
_3327
Position (kb)
3485
3486
3487 Strain fitness (log2 ratio)
-2
-1
0
1 at 3484.640 kb on - strand at 3484.685 kb on - strand at 3484.731 kb on - strand, within HP15_3324 at 3484.800 kb on + strand, within HP15_3324 at 3484.920 kb on - strand, within HP15_3324 at 3485.070 kb on - strand, within HP15_3324 at 3485.206 kb on - strand, within HP15_3324 at 3485.389 kb on - strand, within HP15_3324 at 3485.461 kb on - strand at 3485.525 kb on + strand at 3485.535 kb on + strand at 3485.565 kb on - strand at 3485.777 kb on + strand, within HP15_3325 at 3485.777 kb on + strand, within HP15_3325 at 3485.785 kb on - strand, within HP15_3325 at 3485.787 kb on - strand, within HP15_3325 at 3485.818 kb on + strand, within HP15_3325 at 3485.898 kb on + strand, within HP15_3325 at 3486.058 kb on - strand, within HP15_3325 at 3486.100 kb on + strand, within HP15_3325 at 3486.136 kb on - strand at 3486.281 kb on + strand at 3486.992 kb on - strand at 3487.081 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 3,484,640 - +1.1 3,484,685 - -0.5 3,484,731 - HP15_3324 0.11 -0.8 3,484,800 + HP15_3324 0.19 +1.1 3,484,920 - HP15_3324 0.33 -0.7 3,485,070 - HP15_3324 0.51 +1.1 3,485,206 - HP15_3324 0.66 +1.5 3,485,389 - HP15_3324 0.88 -0.4 3,485,461 - +0.4 3,485,525 + +0.7 3,485,535 + +0.1 3,485,565 - -1.0 3,485,777 + HP15_3325 0.34 -0.6 3,485,777 + HP15_3325 0.34 -0.2 3,485,785 - HP15_3325 0.35 +1.1 3,485,787 - HP15_3325 0.36 +0.0 3,485,818 + HP15_3325 0.41 -1.1 3,485,898 + HP15_3325 0.53 +0.1 3,486,058 - HP15_3325 0.78 -0.7 3,486,100 + HP15_3325 0.85 +0.3 3,486,136 - -0.1 3,486,281 + -2.5 3,486,992 - -0.1 3,487,081 - -0.0
Or see this region's nucleotide sequence