Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3020 and HP15_3021 are separated by 12 nucleotides HP15_3021 and HP15_3022 are separated by 128 nucleotides HP15_3022 and HP15_3023 are separated by 2 nucleotides
HP15_3020: HP15_3020 - lysine exporter protein (LYSE/YGGA), at 3,164,627 to 3,165,247
_3020
HP15_3021: HP15_3021 - glycolate oxidase iron-sulfur subunit, at 3,165,260 to 3,165,691
_3021
HP15_3022: HP15_3022 - protein of unknown function cysteine-rich region domain protein, at 3,165,820 to 3,167,028
_3022
HP15_3023: HP15_3023 - glycolate oxidase FAD binding subunit, at 3,167,031 to 3,168,098
_3023
Position (kb)
3165
3166
3167
3168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3165.000 kb on - strand, within HP15_3020 at 3165.000 kb on - strand, within HP15_3020 at 3165.059 kb on - strand, within HP15_3020 at 3165.064 kb on + strand, within HP15_3020 at 3165.251 kb on - strand at 3165.294 kb on - strand at 3165.398 kb on + strand, within HP15_3021 at 3165.414 kb on + strand, within HP15_3021 at 3165.422 kb on - strand, within HP15_3021 at 3165.422 kb on - strand, within HP15_3021 at 3165.438 kb on + strand, within HP15_3021 at 3165.659 kb on - strand at 3165.697 kb on + strand at 3165.704 kb on + strand at 3165.712 kb on - strand at 3165.712 kb on - strand at 3165.834 kb on + strand at 3166.076 kb on - strand, within HP15_3022 at 3166.146 kb on - strand, within HP15_3022 at 3166.196 kb on - strand, within HP15_3022 at 3166.408 kb on - strand, within HP15_3022 at 3166.416 kb on + strand, within HP15_3022 at 3166.432 kb on - strand, within HP15_3022 at 3166.490 kb on + strand, within HP15_3022 at 3166.490 kb on + strand, within HP15_3022 at 3166.498 kb on - strand, within HP15_3022 at 3166.597 kb on + strand, within HP15_3022 at 3166.669 kb on + strand, within HP15_3022 at 3166.785 kb on - strand at 3166.810 kb on + strand, within HP15_3022 at 3166.839 kb on + strand, within HP15_3022 at 3166.916 kb on - strand at 3167.075 kb on + strand at 3167.083 kb on - strand at 3167.109 kb on + strand at 3167.175 kb on - strand, within HP15_3023 at 3167.180 kb on - strand, within HP15_3023 at 3167.324 kb on - strand, within HP15_3023 at 3167.412 kb on + strand, within HP15_3023 at 3167.456 kb on - strand, within HP15_3023 at 3167.456 kb on - strand, within HP15_3023 at 3167.562 kb on - strand, within HP15_3023 at 3167.562 kb on - strand, within HP15_3023 at 3167.648 kb on - strand, within HP15_3023 at 3167.657 kb on + strand, within HP15_3023 at 3167.657 kb on + strand, within HP15_3023 at 3167.716 kb on + strand, within HP15_3023 at 3167.786 kb on + strand, within HP15_3023 at 3167.806 kb on - strand, within HP15_3023 at 3167.978 kb on - strand, within HP15_3023
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 3,165,000 - HP15_3020 0.60 -1.1 3,165,000 - HP15_3020 0.60 -0.1 3,165,059 - HP15_3020 0.70 +1.1 3,165,064 + HP15_3020 0.70 -0.4 3,165,251 - -0.4 3,165,294 - +0.2 3,165,398 + HP15_3021 0.32 +0.3 3,165,414 + HP15_3021 0.36 -1.0 3,165,422 - HP15_3021 0.38 -0.5 3,165,422 - HP15_3021 0.38 +1.1 3,165,438 + HP15_3021 0.41 +0.7 3,165,659 - -0.3 3,165,697 + +2.0 3,165,704 + +1.6 3,165,712 - -0.0 3,165,712 - -0.2 3,165,834 + -1.5 3,166,076 - HP15_3022 0.21 -0.7 3,166,146 - HP15_3022 0.27 -0.0 3,166,196 - HP15_3022 0.31 -1.8 3,166,408 - HP15_3022 0.49 -1.7 3,166,416 + HP15_3022 0.49 +0.4 3,166,432 - HP15_3022 0.51 -1.1 3,166,490 + HP15_3022 0.55 -2.2 3,166,490 + HP15_3022 0.55 -1.8 3,166,498 - HP15_3022 0.56 -2.1 3,166,597 + HP15_3022 0.64 -0.8 3,166,669 + HP15_3022 0.70 -1.8 3,166,785 - +0.2 3,166,810 + HP15_3022 0.82 -2.0 3,166,839 + HP15_3022 0.84 -0.8 3,166,916 - -2.4 3,167,075 + -0.7 3,167,083 - -1.7 3,167,109 + -1.2 3,167,175 - HP15_3023 0.13 -2.4 3,167,180 - HP15_3023 0.14 -1.2 3,167,324 - HP15_3023 0.27 -1.6 3,167,412 + HP15_3023 0.36 -0.2 3,167,456 - HP15_3023 0.40 -0.5 3,167,456 - HP15_3023 0.40 -2.8 3,167,562 - HP15_3023 0.50 -0.6 3,167,562 - HP15_3023 0.50 -0.4 3,167,648 - HP15_3023 0.58 -0.7 3,167,657 + HP15_3023 0.59 +0.6 3,167,657 + HP15_3023 0.59 -1.2 3,167,716 + HP15_3023 0.64 -0.3 3,167,786 + HP15_3023 0.71 -1.8 3,167,806 - HP15_3023 0.73 -1.8 3,167,978 - HP15_3023 0.89 -1.5
Or see this region's nucleotide sequence