Experiment: L-Glutamine as (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3003 and HP15_3004 are separated by 144 nucleotides HP15_3004 and HP15_3005 are separated by 144 nucleotides HP15_3005 and HP15_3006 are separated by 20 nucleotides
HP15_3003: HP15_3003 - protein-export protein SecB, at 3,148,073 to 3,148,576
_3003
HP15_3004: HP15_3004 - rhodanese domain protein, at 3,148,721 to 3,149,137
_3004
HP15_3005: HP15_3005 - phosphoglyceromutase, at 3,149,282 to 3,150,832
_3005
HP15_3006: HP15_3006 - peptidase M23B, at 3,150,853 to 3,151,986
_3006
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-1
0
1 at 3148.289 kb on + strand, within HP15_3003 at 3148.331 kb on + strand, within HP15_3003 at 3148.388 kb on - strand, within HP15_3003 at 3148.388 kb on - strand, within HP15_3003 at 3148.395 kb on + strand, within HP15_3003 at 3148.470 kb on + strand, within HP15_3003 at 3148.487 kb on + strand, within HP15_3003 at 3148.609 kb on + strand at 3148.628 kb on + strand at 3148.634 kb on - strand at 3148.641 kb on - strand at 3148.877 kb on - strand, within HP15_3004 at 3148.910 kb on + strand, within HP15_3004 at 3148.910 kb on + strand, within HP15_3004 at 3149.139 kb on + strand at 3149.167 kb on + strand at 3149.167 kb on + strand at 3150.825 kb on - strand at 3150.853 kb on + strand at 3150.885 kb on + strand at 3150.908 kb on - strand at 3150.928 kb on - strand at 3150.956 kb on + strand at 3151.012 kb on - strand, within HP15_3006 at 3151.113 kb on + strand, within HP15_3006 at 3151.187 kb on - strand, within HP15_3006 at 3151.268 kb on - strand, within HP15_3006 at 3151.271 kb on + strand, within HP15_3006 at 3151.304 kb on + strand, within HP15_3006 at 3151.340 kb on + strand, within HP15_3006 at 3151.380 kb on + strand, within HP15_3006 at 3151.388 kb on - strand, within HP15_3006 at 3151.390 kb on + strand, within HP15_3006 at 3151.572 kb on - strand, within HP15_3006 at 3151.617 kb on + strand, within HP15_3006 at 3151.625 kb on - strand, within HP15_3006 at 3151.646 kb on - strand, within HP15_3006 at 3151.647 kb on - strand, within HP15_3006 at 3151.729 kb on + strand, within HP15_3006 at 3151.772 kb on + strand, within HP15_3006 at 3151.780 kb on - strand, within HP15_3006 at 3151.780 kb on - strand, within HP15_3006
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine as (N) remove 3,148,289 + HP15_3003 0.43 -0.4 3,148,331 + HP15_3003 0.51 +0.8 3,148,388 - HP15_3003 0.62 -1.7 3,148,388 - HP15_3003 0.62 +0.8 3,148,395 + HP15_3003 0.64 +0.4 3,148,470 + HP15_3003 0.79 -0.5 3,148,487 + HP15_3003 0.82 +0.7 3,148,609 + -0.7 3,148,628 + -1.7 3,148,634 - +0.8 3,148,641 - -1.3 3,148,877 - HP15_3004 0.37 -0.0 3,148,910 + HP15_3004 0.45 -1.6 3,148,910 + HP15_3004 0.45 -0.5 3,149,139 + +0.1 3,149,167 + -0.3 3,149,167 + -0.6 3,150,825 - +0.0 3,150,853 + -0.3 3,150,885 + +0.9 3,150,908 - +1.0 3,150,928 - -0.4 3,150,956 + -0.5 3,151,012 - HP15_3006 0.14 +0.1 3,151,113 + HP15_3006 0.23 -0.3 3,151,187 - HP15_3006 0.29 -0.2 3,151,268 - HP15_3006 0.37 +0.0 3,151,271 + HP15_3006 0.37 +0.1 3,151,304 + HP15_3006 0.40 -0.1 3,151,340 + HP15_3006 0.43 +0.2 3,151,380 + HP15_3006 0.46 -0.8 3,151,388 - HP15_3006 0.47 +0.1 3,151,390 + HP15_3006 0.47 +1.3 3,151,572 - HP15_3006 0.63 +0.7 3,151,617 + HP15_3006 0.67 -0.6 3,151,625 - HP15_3006 0.68 -0.2 3,151,646 - HP15_3006 0.70 -0.2 3,151,647 - HP15_3006 0.70 +0.3 3,151,729 + HP15_3006 0.77 -0.3 3,151,772 + HP15_3006 0.81 -1.7 3,151,780 - HP15_3006 0.82 -1.1 3,151,780 - HP15_3006 0.82 -0.1
Or see this region's nucleotide sequence