Experiment: MoYLS4 with G418 0.031 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0224 and DvMF_0225 are separated by 16 nucleotides DvMF_0225 and DvMF_0226 are separated by 315 nucleotides DvMF_0226 and DvMF_0227 are separated by 242 nucleotides
DvMF_0224: DvMF_0224 - glycine dehydrogenase subunit 1 (RefSeq), at 251,248 to 252,579
_0224
DvMF_0225: DvMF_0225 - glycine cleavage system protein H (RefSeq), at 252,596 to 252,979
_0225
DvMF_0226: DvMF_0226 - response regulator receiver protein (RefSeq), at 253,295 to 253,708
_0226
DvMF_0227: DvMF_0227 - phosphoglucomutase (RefSeq), at 253,951 to 255,600
_0227
Position (kb) 252
253 Strain fitness (log2 ratio) -1
0
1 at 251.601 kb on + strand, within DvMF_0224 at 251.900 kb on + strand, within DvMF_0224 at 252.217 kb on - strand, within DvMF_0224 at 252.302 kb on + strand, within DvMF_0224 at 252.302 kb on + strand, within DvMF_0224 at 252.358 kb on + strand, within DvMF_0224 at 252.550 kb on - strand at 252.552 kb on + strand at 252.552 kb on + strand at 252.552 kb on + strand at 252.552 kb on + strand at 252.560 kb on - strand at 252.679 kb on + strand, within DvMF_0225 at 252.701 kb on - strand, within DvMF_0225 at 252.995 kb on + strand at 253.061 kb on + strand at 253.082 kb on - strand at 253.112 kb on - strand at 253.152 kb on - strand at 253.252 kb on + strand at 253.666 kb on + strand, within DvMF_0226 at 253.833 kb on + strand at 253.908 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with G418 0.031 mg/ml remove 251,601 + DvMF_0224 0.27 +0.6 251,900 + DvMF_0224 0.49 -0.1 252,217 - DvMF_0224 0.73 -0.3 252,302 + DvMF_0224 0.79 -0.3 252,302 + DvMF_0224 0.79 +0.4 252,358 + DvMF_0224 0.83 -0.3 252,550 - -0.7 252,552 + -0.4 252,552 + -0.6 252,552 + -0.3 252,552 + -0.5 252,560 - -0.3 252,679 + DvMF_0225 0.22 -0.3 252,701 - DvMF_0225 0.27 -0.4 252,995 + -0.6 253,061 + -0.5 253,082 - -0.5 253,112 - -0.7 253,152 - -0.2 253,252 + -0.4 253,666 + DvMF_0226 0.90 +0.5 253,833 + -0.5 253,908 + -0.2
Or see this region's nucleotide sequence