Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tdh and Shew_3711 are separated by 88 nucleotides Shew_3711 and Shew_3712 are separated by 17 nucleotides Shew_3712 and Shew_3713 are separated by 132 nucleotides
Shew_3710: tdh - L-threonine 3-dehydrogenase (RefSeq), at 4,414,940 to 4,415,965
tdh
Shew_3711: Shew_3711 - rhodanese domain-containing protein (RefSeq), at 4,416,054 to 4,416,359
_3711
Shew_3712: Shew_3712 - rhomboid family protein (RefSeq), at 4,416,377 to 4,417,213
_3712
Shew_3713: Shew_3713 - isoprenoid biosynthesis protein (RefSeq), at 4,417,346 to 4,417,999
_3713
Position (kb)
4416
4417 Strain fitness (log2 ratio)
-1
0
1 at 4415.262 kb on - strand, within tdh at 4415.451 kb on + strand, within tdh at 4415.648 kb on + strand, within tdh at 4415.674 kb on + strand, within tdh at 4415.733 kb on - strand, within tdh at 4415.968 kb on - strand at 4416.299 kb on + strand, within Shew_3711 at 4416.332 kb on + strand at 4416.421 kb on - strand at 4416.433 kb on + strand at 4416.571 kb on + strand, within Shew_3712 at 4416.788 kb on + strand, within Shew_3712 at 4416.902 kb on - strand, within Shew_3712 at 4416.949 kb on - strand, within Shew_3712 at 4417.036 kb on + strand, within Shew_3712 at 4417.161 kb on + strand at 4417.169 kb on - strand at 4417.178 kb on + strand at 4417.205 kb on - strand at 4417.359 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 4,415,262 - tdh Shew_3710 0.31 +1.0 4,415,451 + tdh Shew_3710 0.50 +1.1 4,415,648 + tdh Shew_3710 0.69 +1.3 4,415,674 + tdh Shew_3710 0.72 +1.5 4,415,733 - tdh Shew_3710 0.77 +1.2 4,415,968 - +0.8 4,416,299 + Shew_3711 0.80 +0.9 4,416,332 + +0.1 4,416,421 - +1.1 4,416,433 + -0.8 4,416,571 + Shew_3712 0.23 +0.1 4,416,788 + Shew_3712 0.49 +0.4 4,416,902 - Shew_3712 0.63 +1.2 4,416,949 - Shew_3712 0.68 -0.1 4,417,036 + Shew_3712 0.79 -0.2 4,417,161 + -0.0 4,417,169 - +0.4 4,417,178 + +0.6 4,417,205 - +0.7 4,417,359 + -0.3
Or see this region's nucleotide sequence