Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3599 and Shew_3600 overlap by 1 nucleotides Shew_3600 and Shew_3601 overlap by 4 nucleotides Shew_3601 and Shew_3602 overlap by 4 nucleotides
Shew_3599: Shew_3599 - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase (RefSeq), at 4,296,340 to 4,298,043
_3599
Shew_3600: Shew_3600 - alpha/beta hydrolase fold (RefSeq), at 4,298,043 to 4,298,840
_3600
Shew_3601: Shew_3601 - O-succinylbenzoate synthase (RefSeq), at 4,298,837 to 4,299,895
_3601
Shew_3602: Shew_3602 - O-succinylbenzoate-CoA ligase (RefSeq), at 4,299,892 to 4,301,406
_3602
Position (kb)
4298
4299
4300 Strain fitness (log2 ratio)
-1
0
1
2
3 at 4297.843 kb on - strand, within Shew_3599 at 4297.858 kb on + strand, within Shew_3599 at 4297.938 kb on - strand at 4297.942 kb on + strand at 4298.650 kb on - strand, within Shew_3600 at 4298.727 kb on - strand, within Shew_3600 at 4299.121 kb on + strand, within Shew_3601 at 4299.139 kb on - strand, within Shew_3601 at 4299.336 kb on + strand, within Shew_3601 at 4299.573 kb on - strand, within Shew_3601 at 4299.872 kb on - strand at 4299.872 kb on - strand at 4299.898 kb on - strand at 4299.955 kb on - strand at 4299.956 kb on + strand at 4300.055 kb on + strand, within Shew_3602 at 4300.061 kb on + strand, within Shew_3602 at 4300.061 kb on + strand, within Shew_3602 at 4300.061 kb on + strand, within Shew_3602 at 4300.278 kb on - strand, within Shew_3602 at 4300.316 kb on + strand, within Shew_3602 at 4300.499 kb on - strand, within Shew_3602 at 4300.569 kb on + strand, within Shew_3602 at 4300.603 kb on + strand, within Shew_3602 at 4300.603 kb on + strand, within Shew_3602 at 4300.611 kb on - strand, within Shew_3602 at 4300.729 kb on - strand, within Shew_3602 at 4300.731 kb on + strand, within Shew_3602
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 4,297,843 - Shew_3599 0.88 +3.2 4,297,858 + Shew_3599 0.89 +0.5 4,297,938 - +1.3 4,297,942 + +1.5 4,298,650 - Shew_3600 0.76 +0.5 4,298,727 - Shew_3600 0.86 +1.5 4,299,121 + Shew_3601 0.27 +0.4 4,299,139 - Shew_3601 0.29 +0.9 4,299,336 + Shew_3601 0.47 +1.0 4,299,573 - Shew_3601 0.69 +0.7 4,299,872 - +0.7 4,299,872 - +0.7 4,299,898 - +1.5 4,299,955 - +0.4 4,299,956 + -0.6 4,300,055 + Shew_3602 0.11 +0.8 4,300,061 + Shew_3602 0.11 +2.1 4,300,061 + Shew_3602 0.11 +1.1 4,300,061 + Shew_3602 0.11 +0.5 4,300,278 - Shew_3602 0.25 +0.7 4,300,316 + Shew_3602 0.28 +1.1 4,300,499 - Shew_3602 0.40 +1.2 4,300,569 + Shew_3602 0.45 +0.7 4,300,603 + Shew_3602 0.47 +1.0 4,300,603 + Shew_3602 0.47 +0.8 4,300,611 - Shew_3602 0.47 +0.5 4,300,729 - Shew_3602 0.55 +0.3 4,300,731 + Shew_3602 0.55 +0.9
Or see this region's nucleotide sequence