Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2822 and Shew_2823 are separated by 195 nucleotides Shew_2823 and rpsO are separated by 107 nucleotides rpsO and Shew_2825 are separated by 95 nucleotides
Shew_2822: Shew_2822 - polynucleotide phosphorylase/polyadenylase (RefSeq), at 3,343,826 to 3,345,928
_2822
Shew_2823: Shew_2823 - diguanylate cyclase/phosphodiesterase (RefSeq), at 3,346,124 to 3,348,157
_2823
Shew_2824: rpsO - 30S ribosomal protein S15 (RefSeq), at 3,348,265 to 3,348,534
rpsO
Shew_2825: Shew_2825 - tRNA pseudouridine synthase B (RefSeq), at 3,348,630 to 3,349,577
_2825
Position (kb)
3346
3347
3348
3349 Strain fitness (log2 ratio)
-1
0
1
2 at 3346.108 kb on + strand at 3346.502 kb on - strand, within Shew_2823 at 3346.633 kb on + strand, within Shew_2823 at 3347.016 kb on + strand, within Shew_2823 at 3347.217 kb on + strand, within Shew_2823 at 3347.225 kb on - strand, within Shew_2823 at 3347.316 kb on - strand, within Shew_2823 at 3347.735 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3347.815 kb on + strand, within Shew_2823 at 3348.775 kb on + strand, within Shew_2825 at 3349.000 kb on + strand, within Shew_2825 at 3349.095 kb on - strand, within Shew_2825
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 3,346,108 + +2.2 3,346,502 - Shew_2823 0.19 -0.2 3,346,633 + Shew_2823 0.25 -0.7 3,347,016 + Shew_2823 0.44 +0.2 3,347,217 + Shew_2823 0.54 -0.3 3,347,225 - Shew_2823 0.54 -0.1 3,347,316 - Shew_2823 0.59 +0.0 3,347,735 + Shew_2823 0.79 +0.5 3,347,815 + Shew_2823 0.83 -0.8 3,347,815 + Shew_2823 0.83 +0.4 3,347,815 + Shew_2823 0.83 -1.0 3,348,775 + Shew_2825 0.15 +0.8 3,349,000 + Shew_2825 0.39 +0.3 3,349,095 - Shew_2825 0.49 -0.2
Or see this region's nucleotide sequence