Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2804 and Shew_2805 are separated by 481 nucleotides Shew_2805 and Shew_2806 are separated by 83 nucleotides Shew_2806 and Shew_2807 are separated by 26 nucleotides
Shew_2804: Shew_2804 - type IV pilus assembly PilZ (RefSeq), at 3,322,456 to 3,322,824
_2804
Shew_2805: Shew_2805 - choloylglycine hydrolase (RefSeq), at 3,323,306 to 3,324,391
_2805
Shew_2806: Shew_2806 - GCN5-related N-acetyltransferase (RefSeq), at 3,324,475 to 3,324,975
_2806
Shew_2807: Shew_2807 - DNA repair protein RadA (RefSeq), at 3,325,002 to 3,326,375
_2807
Position (kb)
3323
3324
3325 Strain fitness (log2 ratio)
-1
0
1
2 at 3322.762 kb on + strand, within Shew_2804 at 3323.142 kb on + strand at 3323.142 kb on + strand at 3323.248 kb on + strand at 3323.377 kb on - strand at 3323.601 kb on + strand, within Shew_2805 at 3324.209 kb on + strand, within Shew_2805 at 3324.255 kb on + strand, within Shew_2805 at 3324.255 kb on + strand, within Shew_2805 at 3324.263 kb on - strand, within Shew_2805 at 3324.301 kb on + strand at 3324.334 kb on + strand at 3324.335 kb on - strand at 3324.342 kb on - strand at 3324.590 kb on - strand, within Shew_2806 at 3324.651 kb on - strand, within Shew_2806 at 3324.797 kb on - strand, within Shew_2806 at 3325.231 kb on - strand, within Shew_2807 at 3325.231 kb on - strand, within Shew_2807 at 3325.239 kb on - strand, within Shew_2807
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 3,322,762 + Shew_2804 0.83 -0.2 3,323,142 + +0.3 3,323,142 + +0.6 3,323,248 + -1.0 3,323,377 - +0.9 3,323,601 + Shew_2805 0.27 -1.1 3,324,209 + Shew_2805 0.83 -1.5 3,324,255 + Shew_2805 0.87 -0.8 3,324,255 + Shew_2805 0.87 -0.1 3,324,263 - Shew_2805 0.88 -0.1 3,324,301 + +0.3 3,324,334 + +0.5 3,324,335 - +0.6 3,324,342 - -0.2 3,324,590 - Shew_2806 0.23 +1.8 3,324,651 - Shew_2806 0.35 -0.8 3,324,797 - Shew_2806 0.64 -0.4 3,325,231 - Shew_2807 0.17 +0.2 3,325,231 - Shew_2807 0.17 -0.5 3,325,239 - Shew_2807 0.17 -0.8
Or see this region's nucleotide sequence