Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0683 and Shew_0684 are separated by 397 nucleotides Shew_0684 and Shew_0685 are separated by 66 nucleotides Shew_0685 and Shew_0686 are separated by 88 nucleotides
Shew_0683: Shew_0683 - secretion protein HlyD family protein (RefSeq), at 777,423 to 778,487
_0683
Shew_0684: Shew_0684 - hypothetical protein (RefSeq), at 778,885 to 779,292
_0684
Shew_0685: Shew_0685 - hypothetical protein (RefSeq), at 779,359 to 780,066
_0685
Shew_0686: Shew_0686 - DSBA oxidoreductase (RefSeq), at 780,155 to 780,817
_0686
Position (kb) 778
779
780 Strain fitness (log2 ratio) -2
-1
0
1 at 777.954 kb on + strand, within Shew_0683 at 778.061 kb on - strand, within Shew_0683 at 778.313 kb on + strand, within Shew_0683 at 778.359 kb on + strand, within Shew_0683 at 778.359 kb on + strand, within Shew_0683 at 778.388 kb on - strand at 778.664 kb on + strand at 778.982 kb on + strand, within Shew_0684 at 779.027 kb on - strand, within Shew_0684 at 779.238 kb on + strand, within Shew_0684 at 779.286 kb on + strand at 779.286 kb on + strand at 779.286 kb on + strand at 779.294 kb on - strand at 779.404 kb on + strand at 779.414 kb on + strand at 779.507 kb on - strand, within Shew_0685 at 779.521 kb on - strand, within Shew_0685 at 779.647 kb on - strand, within Shew_0685 at 779.885 kb on - strand, within Shew_0685
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 777,954 + Shew_0683 0.50 -0.1 778,061 - Shew_0683 0.60 -0.6 778,313 + Shew_0683 0.84 -0.1 778,359 + Shew_0683 0.88 -0.5 778,359 + Shew_0683 0.88 +0.0 778,388 - -0.1 778,664 + +1.1 778,982 + Shew_0684 0.24 -1.0 779,027 - Shew_0684 0.35 -0.1 779,238 + Shew_0684 0.87 -2.6 779,286 + -0.7 779,286 + -1.0 779,286 + +0.0 779,294 - +0.4 779,404 + +0.5 779,414 + -0.3 779,507 - Shew_0685 0.21 -0.2 779,521 - Shew_0685 0.23 -0.7 779,647 - Shew_0685 0.41 +0.1 779,885 - Shew_0685 0.74 -0.1
Or see this region's nucleotide sequence