Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0530 and Shew_0531 are separated by 62 nucleotides Shew_0531 and Shew_0532 are separated by 247 nucleotides
Shew_0530: Shew_0530 - FAD dependent oxidoreductase (RefSeq), at 599,363 to 600,490
_0530
Shew_0531: Shew_0531 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 600,553 to 602,352
_0531
Shew_0532: Shew_0532 - peptidase M28 (RefSeq), at 602,600 to 603,628
_0532
Position (kb) 600
601
602
603 Strain fitness (log2 ratio) -1
0
1
2 at 599.562 kb on - strand, within Shew_0530 at 599.566 kb on + strand, within Shew_0530 at 599.819 kb on - strand, within Shew_0530 at 599.993 kb on - strand, within Shew_0530 at 600.012 kb on + strand, within Shew_0530 at 600.678 kb on - strand at 601.070 kb on + strand, within Shew_0531 at 601.078 kb on - strand, within Shew_0531 at 601.294 kb on - strand, within Shew_0531 at 601.999 kb on - strand, within Shew_0531 at 602.191 kb on - strand at 602.362 kb on - strand at 602.363 kb on - strand at 602.879 kb on - strand, within Shew_0532 at 602.879 kb on - strand, within Shew_0532 at 602.982 kb on + strand, within Shew_0532 at 602.982 kb on + strand, within Shew_0532 at 602.990 kb on - strand, within Shew_0532 at 602.990 kb on - strand, within Shew_0532 at 603.018 kb on + strand, within Shew_0532 at 603.112 kb on - strand, within Shew_0532
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 599,562 - Shew_0530 0.18 +1.3 599,566 + Shew_0530 0.18 -0.2 599,819 - Shew_0530 0.40 +0.5 599,993 - Shew_0530 0.56 -0.9 600,012 + Shew_0530 0.58 -0.1 600,678 - +1.2 601,070 + Shew_0531 0.29 +1.0 601,078 - Shew_0531 0.29 +1.0 601,294 - Shew_0531 0.41 +2.3 601,999 - Shew_0531 0.80 +1.0 602,191 - +1.3 602,362 - +1.3 602,363 - +1.2 602,879 - Shew_0532 0.27 +0.5 602,879 - Shew_0532 0.27 +0.3 602,982 + Shew_0532 0.37 -0.3 602,982 + Shew_0532 0.37 +0.0 602,990 - Shew_0532 0.38 -0.5 602,990 - Shew_0532 0.38 +0.2 603,018 + Shew_0532 0.41 -0.2 603,112 - Shew_0532 0.50 -1.1
Or see this region's nucleotide sequence