Experiment: A22 0.005 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0306 and Shew_R0014 are separated by 218 nucleotides Shew_R0014 and Shew_R0015 are separated by 43 nucleotides Shew_R0015 and Shew_R0016 are separated by 84 nucleotides Shew_R0016 and Shew_R0017 are separated by 52 nucleotides Shew_R0017 and Shew_0307 are separated by 132 nucleotides
Shew_0306: Shew_0306 - diguanylate cyclase/phosphodiesterase with GAF sensor (RefSeq), at 329,116 to 331,764
_0306
Shew_R0014: Shew_R0014 - tRNA-Arg (RefSeq), at 331,983 to 332,059
_R0014
Shew_R0015: Shew_R0015 - tRNA-His (RefSeq), at 332,103 to 332,178
_R0015
Shew_R0016: Shew_R0016 - tRNA-Pro (RefSeq), at 332,263 to 332,339
_R0016
Shew_R0017: Shew_R0017 - tRNA-Pro (RefSeq), at 332,392 to 332,468
_R0017
Shew_0307: Shew_0307 - diguanylate cyclase/phosphodiesterase (RefSeq), at 332,601 to 334,523
_0307
Position (kb) 331
332
333 Strain fitness (log2 ratio) -2
-1
0
1 at 331.026 kb on - strand, within Shew_0306 at 331.080 kb on + strand, within Shew_0306 at 331.084 kb on - strand, within Shew_0306 at 331.094 kb on - strand, within Shew_0306 at 331.099 kb on - strand, within Shew_0306 at 331.164 kb on - strand, within Shew_0306 at 331.176 kb on + strand, within Shew_0306 at 331.407 kb on - strand, within Shew_0306 at 331.484 kb on - strand, within Shew_0306 at 331.986 kb on + strand at 332.850 kb on - strand, within Shew_0307
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.005 mg/ml remove 331,026 - Shew_0306 0.72 -0.2 331,080 + Shew_0306 0.74 +0.4 331,084 - Shew_0306 0.74 -0.1 331,094 - Shew_0306 0.75 -0.3 331,099 - Shew_0306 0.75 +1.2 331,164 - Shew_0306 0.77 +0.4 331,176 + Shew_0306 0.78 +0.1 331,407 - Shew_0306 0.86 +0.4 331,484 - Shew_0306 0.89 +0.2 331,986 + -2.1 332,850 - Shew_0307 0.13 +0.3
Or see this region's nucleotide sequence