Experiment: defined media with 40 mM sodium acetate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1417 and Dsui_1418 overlap by 1 nucleotides Dsui_1418 and Dsui_1419 are separated by 7 nucleotides Dsui_1419 and Dsui_1420 overlap by 4 nucleotides Dsui_1420 and Dsui_1421 are separated by 39 nucleotides Dsui_1421 and Dsui_1422 are separated by 1 nucleotides
Dsui_1417: Dsui_1417 - phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, at 1,473,135 to 1,473,875
_1417
Dsui_1418: Dsui_1418 - imidazoleglycerol phosphate synthase, cyclase subunit, at 1,473,875 to 1,474,636
_1418
Dsui_1419: Dsui_1419 - phosphoribosyl-AMP cyclohydrolase, at 1,474,644 to 1,475,051
_1419
Dsui_1420: Dsui_1420 - phosphoribosyl-ATP pyrophosphohydrolase, at 1,475,048 to 1,475,371
_1420
Dsui_1421: Dsui_1421 - HIT family hydrolase, diadenosine tetraphosphate hydrolase, at 1,475,411 to 1,475,770
_1421
Dsui_1422: Dsui_1422 - twin arginine-targeting protein translocase, TatA/E family, at 1,475,772 to 1,475,996
_1422
Position (kb) 1474
1475
1476 Strain fitness (log2 ratio) -1
0
1 at 1475.406 kb on - strand at 1475.416 kb on - strand at 1475.416 kb on - strand at 1475.463 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.465 kb on + strand, within Dsui_1421 at 1475.473 kb on - strand, within Dsui_1421 at 1475.473 kb on - strand, within Dsui_1421 at 1475.630 kb on + strand, within Dsui_1421 at 1475.990 kb on + strand at 1475.990 kb on + strand at 1475.998 kb on - strand at 1475.998 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 40 mM sodium acetate remove 1,475,406 - -0.1 1,475,416 - +0.6 1,475,416 - +1.0 1,475,463 + Dsui_1421 0.14 -0.3 1,475,465 + Dsui_1421 0.15 -0.4 1,475,465 + Dsui_1421 0.15 -0.6 1,475,465 + Dsui_1421 0.15 -0.3 1,475,465 + Dsui_1421 0.15 -0.1 1,475,473 - Dsui_1421 0.17 -1.5 1,475,473 - Dsui_1421 0.17 +1.5 1,475,630 + Dsui_1421 0.61 -0.3 1,475,990 + -0.5 1,475,990 + -0.0 1,475,998 - -0.1 1,475,998 - -0.4
Or see this region's nucleotide sequence