Experiment: MoYLS4 with Carbenicillin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_3189 and DvMF_3190 overlap by 4 nucleotides DvMF_3190 and DvMF_3191 overlap by 32 nucleotides DvMF_3191 and DvMF_3192 are separated by 3 nucleotides DvMF_3192 and DvMF_3193 are separated by 16 nucleotides
DvMF_3189: DvMF_3189 - NADH dehydrogenase (quinone) (RefSeq), at 4,019,464 to 4,020,597
_3189
DvMF_3190: DvMF_3190 - NADH dehydrogenase (ubiquinone) 30 kDa subunit (RefSeq), at 4,020,594 to 4,021,241
_3190
DvMF_3191: DvMF_3191 - 4Fe-4S ferredoxin iron-sulfur binding domain protein (RefSeq), at 4,021,210 to 4,021,899
_3191
DvMF_3192: DvMF_3192 - NADH ubiquinone oxidoreductase 20 kDa subunit (RefSeq), at 4,021,903 to 4,022,343
_3192
DvMF_3193: DvMF_3193 - respiratory-chain NADH dehydrogenase subunit 1 (RefSeq), at 4,022,360 to 4,023,358
_3193
Position (kb)
4021
4022 Strain fitness (log2 ratio)
-1
0
1
2 at 4020.230 kb on - strand, within DvMF_3189 at 4020.585 kb on + strand at 4020.968 kb on + strand, within DvMF_3190 at 4020.970 kb on - strand, within DvMF_3190 at 4021.207 kb on + strand at 4021.568 kb on - strand, within DvMF_3191 at 4022.171 kb on + strand, within DvMF_3192 at 4022.587 kb on - strand, within DvMF_3193 at 4022.624 kb on + strand, within DvMF_3193
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Carbenicillin 0.0625 mg/ml remove 4,020,230 - DvMF_3189 0.68 +1.4 4,020,585 + -0.2 4,020,968 + DvMF_3190 0.58 +1.0 4,020,970 - DvMF_3190 0.58 +1.3 4,021,207 + +0.9 4,021,568 - DvMF_3191 0.52 +1.4 4,022,171 + DvMF_3192 0.61 +2.3 4,022,587 - DvMF_3193 0.23 -0.1 4,022,624 + DvMF_3193 0.26 +0.5
Or see this region's nucleotide sequence