Experiment: MoYLS4 with Carbenicillin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_2491 and DvMF_2492 are separated by 95 nucleotides DvMF_2492 and DvMF_2493 are separated by 36 nucleotides DvMF_2493 and DvMF_2494 are separated by 37 nucleotides DvMF_2494 and DvMF_2495 are separated by 223 nucleotides DvMF_2495 and DvMF_2496 are separated by 116 nucleotides
DvMF_2491: DvMF_2491 - purine or other phosphorylase family 1 (RefSeq), at 3,145,360 to 3,146,079
_2491
DvMF_2492: DvMF_2492 - hypothetical protein (RefSeq), at 3,146,175 to 3,146,324
_2492
DvMF_2493: DvMF_2493 - hypothetical protein (RefSeq), at 3,146,361 to 3,146,591
_2493
DvMF_2494: DvMF_2494 - nucleotide sugar dehydrogenase (RefSeq), at 3,146,629 to 3,147,945
_2494
DvMF_2495: DvMF_2495 - hypothetical protein (RefSeq), at 3,148,169 to 3,148,375
_2495
DvMF_2496: DvMF_2496 - ubiquinone/menaquinone biosynthesis methyltransferase (RefSeq), at 3,148,492 to 3,149,670
_2496
Position (kb)
3146
3147
3148 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3146.172 kb on - strand at 3146.223 kb on + strand, within DvMF_2492 at 3146.626 kb on + strand at 3146.941 kb on - strand, within DvMF_2494 at 3146.985 kb on + strand, within DvMF_2494 at 3147.335 kb on - strand, within DvMF_2494 at 3147.443 kb on + strand, within DvMF_2494 at 3147.622 kb on + strand, within DvMF_2494 at 3147.651 kb on - strand, within DvMF_2494 at 3147.656 kb on - strand, within DvMF_2494 at 3148.187 kb on + strand at 3148.187 kb on + strand at 3148.562 kb on + strand at 3148.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Carbenicillin 0.0625 mg/ml remove 3,146,172 - -0.7 3,146,223 + DvMF_2492 0.32 -1.2 3,146,626 + -0.8 3,146,941 - DvMF_2494 0.24 -1.8 3,146,985 + DvMF_2494 0.27 -3.4 3,147,335 - DvMF_2494 0.54 -1.6 3,147,443 + DvMF_2494 0.62 -0.1 3,147,622 + DvMF_2494 0.75 -2.1 3,147,651 - DvMF_2494 0.78 -4.2 3,147,656 - DvMF_2494 0.78 -1.3 3,148,187 + -0.5 3,148,187 + -0.6 3,148,562 + -1.0 3,148,589 - -0.3
Or see this region's nucleotide sequence