Experiment: m.b. Chlorate 40 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1141 and Dshi_1142 are separated by 3 nucleotides Dshi_1142 and Dshi_1143 overlap by 4 nucleotides Dshi_1143 and Dshi_1144 are separated by 21 nucleotides Dshi_1144 and Dshi_1145 are separated by 97 nucleotides Dshi_1145 and Dshi_1146 are separated by 13 nucleotides
Dshi_1141: Dshi_1141 - protoheme IX farnesyltransferase (RefSeq), at 1,168,989 to 1,169,975
_1141
Dshi_1142: Dshi_1142 - hypothetical protein (RefSeq), at 1,169,979 to 1,170,164
_1142
Dshi_1143: Dshi_1143 - cytochrome c oxidase assembly protein CtaG/Cox11 (RefSeq), at 1,170,161 to 1,170,772
_1143
Dshi_1144: Dshi_1144 - cytochrome c oxidase subunit III (RefSeq), at 1,170,794 to 1,171,600
_1144
Dshi_1145: Dshi_1145 - hypothetical protein (RefSeq), at 1,171,698 to 1,172,399
_1145
Dshi_1146: Dshi_1146 - threonine synthase (RefSeq), at 1,172,413 to 1,173,804
_1146
Position (kb)
1170
1171
1172 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1169.843 kb on + strand, within Dshi_1141 at 1169.909 kb on + strand at 1170.002 kb on + strand, within Dshi_1142 at 1170.118 kb on + strand, within Dshi_1142 at 1170.673 kb on + strand, within Dshi_1143 at 1171.222 kb on + strand at 1171.331 kb on - strand, within Dshi_1144 at 1171.936 kb on + strand, within Dshi_1145
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Chlorate 40 mM remove 1,169,843 + Dshi_1141 0.87 -1.2 1,169,909 + -0.1 1,170,002 + Dshi_1142 0.12 -2.1 1,170,118 + Dshi_1142 0.75 -2.6 1,170,673 + Dshi_1143 0.84 -2.3 1,171,222 + +2.5 1,171,331 - Dshi_1144 0.67 +0.5 1,171,936 + Dshi_1145 0.34 -3.0
Or see this region's nucleotide sequence