Experiment: defined media with 40 mM sodium acetate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2821 and Dsui_2822 overlap by 4 nucleotides Dsui_2822 and Dsui_2823 are separated by 73 nucleotides Dsui_2823 and Dsui_2824 are separated by 100 nucleotides Dsui_2824 and Dsui_2825 are separated by 143 nucleotides
Dsui_2821: Dsui_2821 - K+ transport system, NAD-binding component, at 2,997,348 to 2,998,385
_2821
Dsui_2822: Dsui_2822 - hypothetical protein, at 2,998,382 to 2,998,951
_2822
Dsui_2823: Dsui_2823 - DNA internalization-related competence protein ComEC/Rec2, at 2,999,025 to 3,000,758
_2823
Dsui_2824: Dsui_2824 - hypothetical protein, at 3,000,859 to 3,000,951
_2824
Dsui_2825: Dsui_2825 - hypothetical protein, at 3,001,095 to 3,004,202
_2825
Position (kb)
2999
3000
3001 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2998.212 kb on - strand, within Dsui_2821 at 2998.323 kb on + strand at 2998.333 kb on + strand at 2998.490 kb on - strand, within Dsui_2822 at 2998.534 kb on - strand, within Dsui_2822 at 2998.647 kb on - strand, within Dsui_2822 at 2998.697 kb on + strand at 2998.939 kb on - strand at 2998.983 kb on + strand at 2998.983 kb on + strand at 2998.991 kb on - strand at 2999.037 kb on + strand at 2999.256 kb on - strand at 2999.747 kb on - strand, within Dsui_2823 at 2999.830 kb on - strand at 3000.198 kb on - strand, within Dsui_2823 at 3000.247 kb on + strand at 3000.266 kb on + strand at 3000.300 kb on - strand, within Dsui_2823 at 3000.399 kb on - strand, within Dsui_2823 at 3000.470 kb on + strand, within Dsui_2823 at 3000.478 kb on - strand at 3000.488 kb on - strand, within Dsui_2823 at 3000.573 kb on - strand, within Dsui_2823 at 3000.575 kb on - strand, within Dsui_2823 at 3000.618 kb on + strand at 3000.618 kb on + strand at 3000.626 kb on - strand at 3000.732 kb on - strand at 3000.781 kb on + strand at 3000.781 kb on - strand at 3000.788 kb on - strand at 3000.789 kb on - strand at 3000.805 kb on + strand at 3000.812 kb on + strand at 3000.813 kb on - strand at 3000.820 kb on - strand at 3000.820 kb on - strand at 3000.820 kb on - strand at 3000.822 kb on + strand at 3000.823 kb on - strand at 3000.823 kb on - strand at 3000.823 kb on - strand at 3000.823 kb on - strand at 3000.830 kb on - strand at 3000.830 kb on - strand at 3000.846 kb on + strand at 3000.931 kb on - strand, within Dsui_2824 at 3000.931 kb on - strand, within Dsui_2824 at 3000.948 kb on - strand at 3001.012 kb on + strand at 3001.049 kb on + strand at 3001.049 kb on + strand at 3001.057 kb on - strand at 3001.066 kb on - strand at 3001.066 kb on - strand at 3001.066 kb on - strand at 3001.096 kb on - strand at 3001.124 kb on - strand at 3001.125 kb on + strand at 3001.133 kb on - strand at 3001.135 kb on - strand at 3001.186 kb on - strand at 3001.186 kb on - strand at 3001.186 kb on - strand at 3001.303 kb on + strand at 3001.303 kb on + strand at 3001.305 kb on + strand at 3001.305 kb on + strand at 3001.308 kb on - strand at 3001.308 kb on - strand at 3001.311 kb on - strand at 3001.367 kb on + strand at 3001.375 kb on - strand at 3001.375 kb on - strand at 3001.401 kb on + strand at 3001.401 kb on + strand at 3001.409 kb on - strand, within Dsui_2825 at 3001.409 kb on - strand, within Dsui_2825 at 3001.522 kb on - strand, within Dsui_2825 at 3001.529 kb on - strand, within Dsui_2825 at 3001.590 kb on + strand, within Dsui_2825 at 3001.639 kb on + strand at 3001.654 kb on + strand, within Dsui_2825 at 3001.662 kb on - strand at 3001.688 kb on + strand, within Dsui_2825 at 3001.733 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 40 mM sodium acetate remove 2,998,212 - Dsui_2821 0.83 +0.7 2,998,323 + +0.5 2,998,333 + +2.1 2,998,490 - Dsui_2822 0.19 -0.2 2,998,534 - Dsui_2822 0.27 -0.1 2,998,647 - Dsui_2822 0.46 -0.2 2,998,697 + +2.1 2,998,939 - +0.6 2,998,983 + +2.1 2,998,983 + +1.1 2,998,991 - +0.3 2,999,037 + +1.1 2,999,256 - +2.1 2,999,747 - Dsui_2823 0.42 +0.9 2,999,830 - +2.5 3,000,198 - Dsui_2823 0.68 +1.1 3,000,247 + +0.5 3,000,266 + +0.1 3,000,300 - Dsui_2823 0.74 -0.9 3,000,399 - Dsui_2823 0.79 -0.5 3,000,470 + Dsui_2823 0.83 -1.8 3,000,478 - +1.6 3,000,488 - Dsui_2823 0.84 -0.3 3,000,573 - Dsui_2823 0.89 -0.3 3,000,575 - Dsui_2823 0.89 -0.3 3,000,618 + -1.1 3,000,618 + +0.1 3,000,626 - -0.9 3,000,732 - -0.3 3,000,781 + +1.1 3,000,781 - -0.5 3,000,788 - -0.5 3,000,789 - +0.1 3,000,805 + -1.1 3,000,812 + +0.1 3,000,813 - -0.8 3,000,820 - +0.2 3,000,820 - +0.7 3,000,820 - -0.8 3,000,822 + +0.8 3,000,823 - -1.3 3,000,823 - +0.6 3,000,823 - -0.1 3,000,823 - -1.9 3,000,830 - +1.1 3,000,830 - +2.5 3,000,846 + -2.4 3,000,931 - Dsui_2824 0.77 -0.2 3,000,931 - Dsui_2824 0.77 +0.1 3,000,948 - +0.1 3,001,012 + +2.1 3,001,049 + +2.1 3,001,049 + -0.3 3,001,057 - +0.1 3,001,066 - -0.8 3,001,066 - -1.3 3,001,066 - +1.1 3,001,096 - +0.1 3,001,124 - +0.2 3,001,125 + +0.1 3,001,133 - +0.7 3,001,135 - -0.3 3,001,186 - -0.1 3,001,186 - +0.1 3,001,186 - +0.5 3,001,303 + -0.8 3,001,303 + +0.1 3,001,305 + +0.6 3,001,305 + +0.7 3,001,308 - +0.5 3,001,308 - -0.4 3,001,311 - -0.7 3,001,367 + -0.2 3,001,375 - -0.6 3,001,375 - -0.1 3,001,401 + +0.5 3,001,401 + +1.1 3,001,409 - Dsui_2825 0.10 -1.9 3,001,409 - Dsui_2825 0.10 +0.2 3,001,522 - Dsui_2825 0.14 +1.2 3,001,529 - Dsui_2825 0.14 -0.1 3,001,590 + Dsui_2825 0.16 -1.4 3,001,639 + +0.7 3,001,654 + Dsui_2825 0.18 +0.9 3,001,662 - +2.6 3,001,688 + Dsui_2825 0.19 -0.3 3,001,733 + +1.1
Or see this region's nucleotide sequence