Experiment: defined media with 40 mM sodium acetate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2297 and Dsui_2298 are separated by 65 nucleotides Dsui_2298 and Dsui_2299 are separated by 114 nucleotides Dsui_2299 and Dsui_2300 are separated by 57 nucleotides Dsui_2300 and Dsui_2301 are separated by 180 nucleotides
Dsui_2297: Dsui_2297 - acetoacetyl-CoA synthase, at 2,450,906 to 2,452,876
_2297
Dsui_2298: Dsui_2298 - putative thioesterase, at 2,452,942 to 2,453,352
_2298
Dsui_2299: Dsui_2299 - flavin-dependent dehydrogenase, at 2,453,467 to 2,455,113
_2299
Dsui_2300: Dsui_2300 - hypothetical protein, at 2,455,171 to 2,455,863
_2300
Dsui_2301: Dsui_2301 - diguanylate cyclase (GGDEF) domain-containing protein, at 2,456,044 to 2,458,458
_2301
Position (kb)
2453
2454
2455
2456 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2452.518 kb on + strand at 2452.687 kb on - strand at 2452.721 kb on - strand at 2452.905 kb on + strand at 2452.918 kb on - strand at 2452.929 kb on - strand at 2452.947 kb on - strand at 2453.167 kb on - strand, within Dsui_2298 at 2453.199 kb on + strand, within Dsui_2298 at 2453.207 kb on + strand at 2453.212 kb on + strand, within Dsui_2298 at 2453.215 kb on - strand, within Dsui_2298 at 2453.244 kb on + strand, within Dsui_2298 at 2453.265 kb on - strand, within Dsui_2298 at 2453.272 kb on + strand, within Dsui_2298 at 2453.275 kb on - strand, within Dsui_2298 at 2453.368 kb on + strand at 2453.368 kb on + strand at 2453.368 kb on + strand at 2453.376 kb on - strand at 2453.376 kb on - strand at 2453.645 kb on - strand at 2453.650 kb on + strand, within Dsui_2299 at 2453.653 kb on - strand, within Dsui_2299 at 2453.828 kb on - strand at 2454.004 kb on + strand, within Dsui_2299 at 2454.013 kb on - strand at 2454.037 kb on + strand, within Dsui_2299 at 2454.090 kb on + strand, within Dsui_2299 at 2454.093 kb on + strand, within Dsui_2299 at 2454.093 kb on + strand, within Dsui_2299 at 2454.162 kb on + strand, within Dsui_2299 at 2454.167 kb on + strand, within Dsui_2299 at 2454.205 kb on + strand, within Dsui_2299 at 2454.223 kb on - strand, within Dsui_2299 at 2454.388 kb on + strand, within Dsui_2299 at 2454.415 kb on - strand at 2454.468 kb on + strand, within Dsui_2299 at 2454.500 kb on + strand, within Dsui_2299 at 2454.579 kb on + strand, within Dsui_2299 at 2454.579 kb on + strand, within Dsui_2299 at 2454.617 kb on - strand at 2454.752 kb on + strand, within Dsui_2299 at 2454.754 kb on + strand, within Dsui_2299 at 2454.762 kb on + strand, within Dsui_2299 at 2454.770 kb on - strand, within Dsui_2299 at 2454.871 kb on - strand, within Dsui_2299 at 2454.873 kb on + strand, within Dsui_2299 at 2454.933 kb on + strand, within Dsui_2299 at 2455.006 kb on + strand at 2455.023 kb on + strand at 2455.023 kb on + strand at 2455.179 kb on - strand at 2455.184 kb on + strand at 2455.192 kb on - strand at 2455.210 kb on + strand at 2455.210 kb on + strand at 2455.238 kb on - strand at 2455.303 kb on + strand, within Dsui_2300 at 2455.370 kb on + strand at 2455.437 kb on + strand, within Dsui_2300 at 2455.464 kb on + strand, within Dsui_2300 at 2455.464 kb on + strand, within Dsui_2300 at 2455.590 kb on - strand, within Dsui_2300 at 2455.604 kb on + strand, within Dsui_2300 at 2455.647 kb on - strand, within Dsui_2300 at 2455.666 kb on + strand, within Dsui_2300 at 2455.666 kb on - strand, within Dsui_2300 at 2455.721 kb on - strand at 2455.772 kb on - strand, within Dsui_2300 at 2455.773 kb on - strand at 2455.848 kb on + strand at 2455.901 kb on + strand at 2455.901 kb on + strand at 2455.911 kb on + strand at 2455.919 kb on - strand at 2455.919 kb on - strand at 2456.015 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction defined media with 40 mM sodium acetate remove 2,452,518 + -0.2 2,452,687 - -1.1 2,452,721 - -1.9 2,452,905 + +1.1 2,452,918 - +0.5 2,452,929 - +0.3 2,452,947 - +0.0 2,453,167 - Dsui_2298 0.55 -0.4 2,453,199 + Dsui_2298 0.63 -1.4 2,453,207 + -0.2 2,453,212 + Dsui_2298 0.66 -0.6 2,453,215 - Dsui_2298 0.66 +0.1 2,453,244 + Dsui_2298 0.73 +0.8 2,453,265 - Dsui_2298 0.79 -0.7 2,453,272 + Dsui_2298 0.80 -0.1 2,453,275 - Dsui_2298 0.81 -0.2 2,453,368 + +0.3 2,453,368 + -0.5 2,453,368 + +2.1 2,453,376 - +0.5 2,453,376 - +0.9 2,453,645 - +1.1 2,453,650 + Dsui_2299 0.11 -2.1 2,453,653 - Dsui_2299 0.11 +0.9 2,453,828 - +2.1 2,454,004 + Dsui_2299 0.33 -1.0 2,454,013 - +1.1 2,454,037 + Dsui_2299 0.35 -1.2 2,454,090 + Dsui_2299 0.38 -0.2 2,454,093 + Dsui_2299 0.38 -0.4 2,454,093 + Dsui_2299 0.38 -0.0 2,454,162 + Dsui_2299 0.42 -0.5 2,454,167 + Dsui_2299 0.43 -1.1 2,454,205 + Dsui_2299 0.45 -0.4 2,454,223 - Dsui_2299 0.46 +1.2 2,454,388 + Dsui_2299 0.56 -1.4 2,454,415 - +0.1 2,454,468 + Dsui_2299 0.61 -2.7 2,454,500 + Dsui_2299 0.63 -0.4 2,454,579 + Dsui_2299 0.68 -0.5 2,454,579 + Dsui_2299 0.68 -0.2 2,454,617 - +1.1 2,454,752 + Dsui_2299 0.78 +1.1 2,454,754 + Dsui_2299 0.78 -1.3 2,454,762 + Dsui_2299 0.79 -0.2 2,454,770 - Dsui_2299 0.79 -1.7 2,454,871 - Dsui_2299 0.85 -1.5 2,454,873 + Dsui_2299 0.85 -0.5 2,454,933 + Dsui_2299 0.89 -0.4 2,455,006 + -0.5 2,455,023 + -0.5 2,455,023 + -0.9 2,455,179 - +3.7 2,455,184 + +0.6 2,455,192 - +1.6 2,455,210 + +0.2 2,455,210 + +0.8 2,455,238 - -0.2 2,455,303 + Dsui_2300 0.19 +0.1 2,455,370 + -1.1 2,455,437 + Dsui_2300 0.38 -1.0 2,455,464 + Dsui_2300 0.42 +0.3 2,455,464 + Dsui_2300 0.42 -1.2 2,455,590 - Dsui_2300 0.60 -0.2 2,455,604 + Dsui_2300 0.62 -0.8 2,455,647 - Dsui_2300 0.69 +0.5 2,455,666 + Dsui_2300 0.71 +0.2 2,455,666 - Dsui_2300 0.71 +0.6 2,455,721 - +0.1 2,455,772 - Dsui_2300 0.87 -0.5 2,455,773 - +1.1 2,455,848 + -0.7 2,455,901 + +0.9 2,455,901 + +0.7 2,455,911 + +0.4 2,455,919 - +1.5 2,455,919 - +1.1 2,456,015 + -0.0
Or see this region's nucleotide sequence