Experiment: MoYLS4 with Carbenicillin 0.0625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DvMF_0346 and DvMF_0347 are separated by 163 nucleotides DvMF_0347 and DvMF_0348 are separated by 199 nucleotides DvMF_0348 and secG are separated by 38 nucleotides secG and DvMF_R0017 are separated by 36 nucleotides DvMF_R0017 and DvMF_0350 are separated by 200 nucleotides
DvMF_0346: DvMF_0346 - NUDIX hydrolase (RefSeq), at 405,236 to 405,784
_0346
DvMF_0347: DvMF_0347 - ribosomal-protein-alanine acetyltransferase (RefSeq), at 405,948 to 406,526
_0347
DvMF_0348: DvMF_0348 - Triose-phosphate isomerase (RefSeq), at 406,726 to 407,481
_0348
DvMF_0349: secG - preprotein translocase subunit SecG (RefSeq), at 407,520 to 407,864
secG
DvMF_R0017: DvMF_R0017 - tRNA-Leu (RefSeq), at 407,901 to 407,987
_R0017
DvMF_0350: DvMF_0350 - extracellular solute-binding protein family 3 (RefSeq), at 408,188 to 409,153
_0350
Position (kb) 406
407
408 Strain fitness (log2 ratio) -1
0
1 at 405.809 kb on - strand at 405.809 kb on - strand at 405.953 kb on + strand at 406.241 kb on - strand, within DvMF_0347 at 406.473 kb on - strand at 406.511 kb on + strand at 406.529 kb on - strand at 406.529 kb on - strand at 406.646 kb on - strand at 406.696 kb on + strand at 406.714 kb on + strand at 407.179 kb on + strand, within DvMF_0348 at 407.238 kb on + strand, within DvMF_0348 at 408.258 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Carbenicillin 0.0625 mg/ml remove 405,809 - -0.1 405,809 - -1.2 405,953 + -0.3 406,241 - DvMF_0347 0.51 -0.2 406,473 - -0.1 406,511 + -0.2 406,529 - -0.6 406,529 - -0.3 406,646 - -0.5 406,696 + -0.0 406,714 + -0.7 407,179 + DvMF_0348 0.60 +0.4 407,238 + DvMF_0348 0.68 -1.4 408,258 + -0.6
Or see this region's nucleotide sequence