Strain Fitness in Shewanella oneidensis MR-1 around SO0293

Experiment: nitrite 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSO0291 and rpe are separated by 262 nucleotidesrpe and gph overlap by 1 nucleotidesgph and trpS are separated by 36 nucleotides SO0291: SO0291 - hypothetical protein (NCBI ptt file), at 297,978 to 298,343 SO0291 SO0292: rpe - ribulose-phosphate 3-epimerase (NCBI ptt file), at 298,606 to 299,283 rpe SO0293: gph - phosphoglycolate phosphatase (NCBI ptt file), at 299,283 to 299,966 gph SO0294: trpS - tryptophanyl-tRNA synthetase (NCBI ptt file), at 300,003 to 301,001 trpS Position (kb) 299 300Strain fitness (log2 ratio) -1 0 1at 298.500 kb on + strandat 298.540 kb on + strandat 299.000 kb on + strand, within rpeat 299.411 kb on + strand, within gphat 299.419 kb on - strand, within gphat 299.436 kb on + strand, within gphat 299.574 kb on - strand, within gphat 299.608 kb on + strand, within gphat 299.638 kb on - strand, within gphat 299.638 kb on - strand, within gphat 299.725 kb on - strand, within gph

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Per-strain Table

Position Strand Gene LocusTag Fraction nitrite 5 mM
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298,500 + +0.5
298,540 + +0.1
299,000 + rpe SO0292 0.58 -1.5
299,411 + gph SO0293 0.19 -0.9
299,419 - gph SO0293 0.20 -0.1
299,436 + gph SO0293 0.22 -1.0
299,574 - gph SO0293 0.43 -0.1
299,608 + gph SO0293 0.48 -1.3
299,638 - gph SO0293 0.52 -0.4
299,638 - gph SO0293 0.52 -1.0
299,725 - gph SO0293 0.65 -0.6

Or see this region's nucleotide sequence