Experiment: MoYLS4 with Carbenicillin 0.0468 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsU and DvMF_0499 are separated by 195 nucleotides DvMF_0499 and DvMF_0500 are separated by 15 nucleotides DvMF_0500 and ksgA are separated by 191 nucleotides ksgA and DvMF_0502 are separated by 197 nucleotides DvMF_0502 and DvMF_0503 are separated by 57 nucleotides
DvMF_0498: rpsU - 30S ribosomal protein S21 (RefSeq), at 586,768 to 586,971
rpsU
DvMF_0499: DvMF_0499 - hypothetical protein (RefSeq), at 587,167 to 587,355
_0499
DvMF_0500: DvMF_0500 - histone family protein DNA-binding protein (RefSeq), at 587,371 to 587,643
_0500
DvMF_0501: ksgA - dimethyladenosine transferase (RefSeq), at 587,835 to 588,647
ksgA
DvMF_0502: DvMF_0502 - hypothetical protein (RefSeq), at 588,845 to 589,477
_0502
DvMF_0503: DvMF_0503 - elongation factor G (RefSeq), at 589,535 to 591,631
_0503
Position (kb) 587
588
589 Strain fitness (log2 ratio) -1
0
1 at 587.057 kb on - strand at 587.178 kb on - strand at 587.259 kb on + strand, within DvMF_0499 at 587.482 kb on + strand, within DvMF_0500 at 587.514 kb on + strand, within DvMF_0500 at 587.522 kb on - strand, within DvMF_0500 at 587.590 kb on - strand, within DvMF_0500 at 587.633 kb on + strand at 587.792 kb on - strand at 587.792 kb on - strand at 588.137 kb on + strand, within ksgA at 588.792 kb on + strand at 589.392 kb on - strand, within DvMF_0502 at 589.458 kb on + strand at 589.491 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Carbenicillin 0.0468 mg/ml remove 587,057 - +0.1 587,178 - -0.4 587,259 + DvMF_0499 0.49 -0.4 587,482 + DvMF_0500 0.41 -0.2 587,514 + DvMF_0500 0.52 -0.1 587,522 - DvMF_0500 0.55 +0.2 587,590 - DvMF_0500 0.80 -0.7 587,633 + -0.0 587,792 - -0.1 587,792 - -0.1 588,137 + ksgA DvMF_0501 0.37 -1.0 588,792 + -0.3 589,392 - DvMF_0502 0.86 +0.2 589,458 + -0.7 589,491 + -0.1
Or see this region's nucleotide sequence