Experiment: L-Serine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pheT and ihfA are separated by 3 nucleotides ihfA and Shewana3_1866 are separated by 96 nucleotides Shewana3_1866 and Shewana3_1867 are separated by 129 nucleotides Shewana3_1867 and Shewana3_1868 overlap by 1 nucleotides
Shewana3_1864: pheT - phenylalanyl-tRNA synthetase subunit beta (RefSeq), at 2,208,269 to 2,210,656
pheT
Shewana3_1865: ihfA - integration host factor subunit alpha (RefSeq), at 2,210,660 to 2,210,956
ihfA
Shewana3_1866: Shewana3_1866 - lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase (RefSeq), at 2,211,053 to 2,211,991
_1866
Shewana3_1867: Shewana3_1867 - hydrogenase nickel insertion protein HypA (RefSeq), at 2,212,121 to 2,212,477
_1867
Shewana3_1868: Shewana3_1868 - hydrogenase expression/formation protein HypE (RefSeq), at 2,212,477 to 2,213,496
_1868
Position (kb)
2211
2212 Strain fitness (log2 ratio)
-1
0
1 at 2211.196 kb on - strand at 2211.523 kb on + strand, within Shewana3_1866 at 2211.569 kb on + strand, within Shewana3_1866 at 2212.204 kb on - strand, within Shewana3_1867 at 2212.209 kb on + strand, within Shewana3_1867 at 2212.494 kb on - strand at 2212.520 kb on - strand at 2212.649 kb on - strand, within Shewana3_1868 at 2212.717 kb on + strand, within Shewana3_1868 at 2212.721 kb on - strand, within Shewana3_1868
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (C) remove 2,211,196 - -1.0 2,211,523 + Shewana3_1866 0.50 -0.5 2,211,569 + Shewana3_1866 0.55 +1.1 2,212,204 - Shewana3_1867 0.23 +1.0 2,212,209 + Shewana3_1867 0.25 +0.3 2,212,494 - -1.2 2,212,520 - +0.7 2,212,649 - Shewana3_1868 0.17 -0.3 2,212,717 + Shewana3_1868 0.24 +0.4 2,212,721 - Shewana3_1868 0.24 +0.5
Or see this region's nucleotide sequence