Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3998 and Dshi_3999 are separated by 80 nucleotides Dshi_3999 and Dshi_4000 are separated by 124 nucleotides Dshi_4000 and Dshi_4001 are separated by 100 nucleotides Dshi_4001 and Dshi_4002 are separated by 1 nucleotides
Dshi_3998: Dshi_3998 - DNA polymerase III, alpha subunit (RefSeq), at 60,274 to 63,624
_3998
Dshi_3999: Dshi_3999 - ParB-like nuclease (RefSeq), at 63,705 to 64,433
_3999
Dshi_4000: Dshi_4000 - hypothetical protein (RefSeq), at 64,558 to 64,860
_4000
Dshi_4001: Dshi_4001 - hypothetical protein (RefSeq), at 64,961 to 65,422
_4001
Dshi_4002: Dshi_4002 - hypothetical protein (RefSeq), at 65,424 to 65,810
_4002
Position (kb) 63
64
65 Strain fitness (log2 ratio) -2
-1
0
1
2 at 62.776 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on + strand, within Dshi_3998 at 62.789 kb on - strand, within Dshi_3998 at 62.797 kb on - strand, within Dshi_3998 at 62.797 kb on - strand, within Dshi_3998 at 62.901 kb on + strand, within Dshi_3998 at 63.193 kb on - strand, within Dshi_3998 at 63.276 kb on + strand, within Dshi_3998 at 63.343 kb on + strand at 63.396 kb on + strand at 63.534 kb on + strand at 63.565 kb on + strand at 63.581 kb on - strand at 63.788 kb on - strand, within Dshi_3999 at 63.797 kb on + strand, within Dshi_3999 at 63.800 kb on - strand, within Dshi_3999 at 63.805 kb on - strand, within Dshi_3999 at 63.840 kb on - strand, within Dshi_3999 at 63.921 kb on + strand, within Dshi_3999 at 64.131 kb on + strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.139 kb on - strand, within Dshi_3999 at 64.158 kb on - strand, within Dshi_3999 at 64.173 kb on - strand, within Dshi_3999 at 64.192 kb on + strand, within Dshi_3999 at 64.235 kb on - strand, within Dshi_3999 at 64.252 kb on + strand, within Dshi_3999 at 64.276 kb on + strand, within Dshi_3999 at 64.341 kb on - strand, within Dshi_3999 at 64.420 kb on - strand at 64.633 kb on - strand, within Dshi_4000 at 64.694 kb on - strand, within Dshi_4000 at 64.761 kb on + strand, within Dshi_4000 at 64.807 kb on - strand, within Dshi_4000 at 64.838 kb on + strand at 64.846 kb on - strand at 65.056 kb on + strand, within Dshi_4001 at 65.056 kb on + strand, within Dshi_4001 at 65.064 kb on - strand, within Dshi_4001 at 65.086 kb on + strand, within Dshi_4001 at 65.134 kb on + strand, within Dshi_4001 at 65.148 kb on + strand, within Dshi_4001 at 65.239 kb on + strand, within Dshi_4001 at 65.239 kb on - strand, within Dshi_4001 at 65.281 kb on - strand, within Dshi_4001 at 65.309 kb on - strand, within Dshi_4001 at 65.360 kb on - strand, within Dshi_4001
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 62,776 + Dshi_3998 0.75 +0.2 62,789 + Dshi_3998 0.75 -0.4 62,789 + Dshi_3998 0.75 +0.0 62,789 + Dshi_3998 0.75 +0.0 62,789 + Dshi_3998 0.75 -0.1 62,789 + Dshi_3998 0.75 -0.1 62,789 - Dshi_3998 0.75 +0.4 62,797 - Dshi_3998 0.75 -1.2 62,797 - Dshi_3998 0.75 -1.5 62,901 + Dshi_3998 0.78 -1.2 63,193 - Dshi_3998 0.87 -0.1 63,276 + Dshi_3998 0.90 -1.6 63,343 + -0.5 63,396 + -0.5 63,534 + -0.9 63,565 + -1.1 63,581 - -1.6 63,788 - Dshi_3999 0.11 -1.1 63,797 + Dshi_3999 0.13 -0.3 63,800 - Dshi_3999 0.13 -0.9 63,805 - Dshi_3999 0.14 -0.7 63,840 - Dshi_3999 0.19 +2.0 63,921 + Dshi_3999 0.30 -0.8 64,131 + Dshi_3999 0.58 +0.4 64,139 - Dshi_3999 0.60 -1.5 64,139 - Dshi_3999 0.60 -0.8 64,139 - Dshi_3999 0.60 +0.3 64,139 - Dshi_3999 0.60 -1.6 64,139 - Dshi_3999 0.60 -1.3 64,158 - Dshi_3999 0.62 +0.1 64,173 - Dshi_3999 0.64 -0.1 64,192 + Dshi_3999 0.67 +1.5 64,235 - Dshi_3999 0.73 +0.9 64,252 + Dshi_3999 0.75 -1.3 64,276 + Dshi_3999 0.78 +0.7 64,341 - Dshi_3999 0.87 -1.1 64,420 - -1.0 64,633 - Dshi_4000 0.25 -0.2 64,694 - Dshi_4000 0.45 -0.2 64,761 + Dshi_4000 0.67 +1.2 64,807 - Dshi_4000 0.82 +0.6 64,838 + +1.0 64,846 - -0.5 65,056 + Dshi_4001 0.21 +0.6 65,056 + Dshi_4001 0.21 +1.3 65,064 - Dshi_4001 0.22 -0.5 65,086 + Dshi_4001 0.27 +0.2 65,134 + Dshi_4001 0.37 -2.0 65,148 + Dshi_4001 0.40 +1.3 65,239 + Dshi_4001 0.60 -1.5 65,239 - Dshi_4001 0.60 -1.9 65,281 - Dshi_4001 0.69 -0.8 65,309 - Dshi_4001 0.75 -0.8 65,360 - Dshi_4001 0.86 -1.2
Or see this region's nucleotide sequence