Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3796 and Dshi_3797 overlap by 4 nucleotides Dshi_3797 and Dshi_3798 overlap by 4 nucleotides Dshi_3798 and Dshi_3799 are separated by 125 nucleotides Dshi_3799 and Dshi_3800 are separated by 4 nucleotides
Dshi_3796: Dshi_3796 - oligopeptide/dipeptide ABC transporter, ATPase subunit (RefSeq), at 4,738 to 5,739
_3796
Dshi_3797: Dshi_3797 - Asp/Glu racemase (RefSeq), at 5,736 to 6,419
_3797
Dshi_3798: Dshi_3798 - NADH:ubiquinone oxidoreductase complex I intermediate-associated protein 30 (RefSeq), at 6,416 to 6,958
_3798
Dshi_3799: Dshi_3799 - HupE/UreJ protein (RefSeq), at 7,084 to 7,680
_3799
Dshi_3800: Dshi_3800 - hypothetical protein (RefSeq), at 7,685 to 8,299
_3800
Position (kb) 6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5.458 kb on - strand, within Dshi_3796 at 5.607 kb on + strand, within Dshi_3796 at 5.769 kb on - strand at 5.772 kb on + strand at 5.803 kb on - strand at 5.853 kb on - strand at 5.921 kb on + strand, within Dshi_3797 at 5.931 kb on + strand, within Dshi_3797 at 5.939 kb on - strand, within Dshi_3797 at 5.944 kb on + strand, within Dshi_3797 at 5.968 kb on + strand, within Dshi_3797 at 6.091 kb on - strand, within Dshi_3797 at 6.269 kb on - strand, within Dshi_3797 at 6.458 kb on + strand at 6.560 kb on + strand, within Dshi_3798 at 6.680 kb on - strand, within Dshi_3798 at 6.761 kb on - strand, within Dshi_3798 at 6.909 kb on - strand at 6.914 kb on - strand at 6.965 kb on + strand at 7.195 kb on + strand, within Dshi_3799 at 7.251 kb on + strand, within Dshi_3799 at 7.393 kb on + strand, within Dshi_3799 at 7.633 kb on - strand at 7.690 kb on - strand at 7.874 kb on - strand, within Dshi_3800
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 5,458 - Dshi_3796 0.72 +0.2 5,607 + Dshi_3796 0.87 -0.5 5,769 - +0.5 5,772 + -2.5 5,803 - +0.5 5,853 - -0.5 5,921 + Dshi_3797 0.27 -0.7 5,931 + Dshi_3797 0.29 +0.2 5,939 - Dshi_3797 0.30 +1.0 5,944 + Dshi_3797 0.30 -1.9 5,968 + Dshi_3797 0.34 -0.5 6,091 - Dshi_3797 0.52 -0.5 6,269 - Dshi_3797 0.78 -0.9 6,458 + -3.0 6,560 + Dshi_3798 0.27 +0.9 6,680 - Dshi_3798 0.49 +2.8 6,761 - Dshi_3798 0.64 +1.0 6,909 - -0.5 6,914 - -0.9 6,965 + +0.2 7,195 + Dshi_3799 0.19 -1.5 7,251 + Dshi_3799 0.28 -0.4 7,393 + Dshi_3799 0.52 -0.8 7,633 - -1.1 7,690 - -0.0 7,874 - Dshi_3800 0.31 +0.5
Or see this region's nucleotide sequence