Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_3172 and Dshi_3173 overlap by 26 nucleotides Dshi_3173 and Dshi_3174 are separated by 1 nucleotides Dshi_3174 and Dshi_3175 overlap by 4 nucleotides Dshi_3175 and Dshi_3176 overlap by 4 nucleotides
Dshi_3172: Dshi_3172 - cytochrome d1 heme region (RefSeq), at 3,334,742 to 3,336,295
_3172
Dshi_3173: Dshi_3173 - Radical SAM domain protein (RefSeq), at 3,336,270 to 3,337,484
_3173
Dshi_3174: Dshi_3174 - putative transcriptional regulator, AsnC family (RefSeq), at 3,337,486 to 3,337,971
_3174
Dshi_3175: Dshi_3175 - putative transcriptional regulator, AsnC family (RefSeq), at 3,337,968 to 3,338,417
_3175
Dshi_3176: Dshi_3176 - putative transcriptional regulator, AsnC family (RefSeq), at 3,338,414 to 3,339,409
_3176
Position (kb)
3336
3337
3338 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3335.286 kb on + strand, within Dshi_3172 at 3335.335 kb on + strand, within Dshi_3172 at 3335.343 kb on - strand, within Dshi_3172 at 3335.343 kb on - strand, within Dshi_3172 at 3335.379 kb on - strand, within Dshi_3172 at 3335.379 kb on - strand, within Dshi_3172 at 3335.412 kb on - strand, within Dshi_3172 at 3335.615 kb on + strand, within Dshi_3172 at 3335.616 kb on + strand, within Dshi_3172 at 3335.623 kb on - strand, within Dshi_3172 at 3335.624 kb on - strand, within Dshi_3172 at 3335.689 kb on - strand, within Dshi_3172 at 3335.708 kb on - strand, within Dshi_3172 at 3335.836 kb on + strand, within Dshi_3172 at 3335.836 kb on + strand, within Dshi_3172 at 3335.836 kb on + strand, within Dshi_3172 at 3335.844 kb on - strand, within Dshi_3172 at 3335.903 kb on + strand, within Dshi_3172 at 3335.916 kb on + strand, within Dshi_3172 at 3335.916 kb on + strand, within Dshi_3172 at 3336.012 kb on + strand, within Dshi_3172 at 3336.015 kb on - strand, within Dshi_3172 at 3336.100 kb on - strand, within Dshi_3172 at 3336.118 kb on - strand, within Dshi_3172 at 3336.123 kb on - strand, within Dshi_3172 at 3336.132 kb on + strand, within Dshi_3172 at 3336.160 kb on + strand at 3336.162 kb on - strand at 3336.364 kb on + strand at 3336.381 kb on + strand at 3336.388 kb on + strand at 3336.415 kb on - strand, within Dshi_3173 at 3336.509 kb on + strand, within Dshi_3173 at 3336.534 kb on - strand, within Dshi_3173 at 3336.551 kb on + strand, within Dshi_3173 at 3336.567 kb on + strand, within Dshi_3173 at 3336.586 kb on + strand, within Dshi_3173 at 3336.672 kb on + strand, within Dshi_3173 at 3336.767 kb on + strand, within Dshi_3173 at 3336.771 kb on + strand, within Dshi_3173 at 3336.780 kb on - strand, within Dshi_3173 at 3336.842 kb on - strand, within Dshi_3173 at 3336.877 kb on + strand, within Dshi_3173 at 3336.877 kb on + strand, within Dshi_3173 at 3336.877 kb on + strand, within Dshi_3173 at 3336.882 kb on + strand, within Dshi_3173 at 3336.904 kb on - strand, within Dshi_3173 at 3336.930 kb on - strand, within Dshi_3173 at 3336.952 kb on + strand, within Dshi_3173 at 3336.983 kb on + strand, within Dshi_3173 at 3336.988 kb on + strand, within Dshi_3173 at 3337.080 kb on + strand, within Dshi_3173 at 3337.144 kb on - strand, within Dshi_3173 at 3337.193 kb on - strand, within Dshi_3173 at 3337.249 kb on + strand, within Dshi_3173 at 3337.253 kb on + strand, within Dshi_3173 at 3337.257 kb on - strand, within Dshi_3173 at 3337.267 kb on + strand, within Dshi_3173 at 3337.276 kb on - strand, within Dshi_3173 at 3337.310 kb on - strand, within Dshi_3173 at 3337.341 kb on + strand, within Dshi_3173 at 3337.381 kb on - strand at 3337.398 kb on + strand at 3337.409 kb on + strand at 3337.417 kb on - strand at 3337.447 kb on - strand at 3337.465 kb on - strand at 3337.465 kb on - strand at 3337.474 kb on + strand at 3337.517 kb on - strand at 3337.535 kb on + strand, within Dshi_3174 at 3337.606 kb on - strand, within Dshi_3174 at 3337.662 kb on - strand, within Dshi_3174 at 3337.739 kb on - strand, within Dshi_3174 at 3337.748 kb on + strand, within Dshi_3174 at 3337.754 kb on - strand, within Dshi_3174 at 3337.754 kb on - strand, within Dshi_3174 at 3337.765 kb on - strand, within Dshi_3174 at 3337.790 kb on - strand, within Dshi_3174 at 3337.830 kb on + strand, within Dshi_3174 at 3337.830 kb on + strand, within Dshi_3174 at 3337.838 kb on - strand, within Dshi_3174 at 3337.838 kb on - strand, within Dshi_3174 at 3337.838 kb on - strand, within Dshi_3174 at 3337.907 kb on + strand, within Dshi_3174 at 3337.929 kb on + strand at 3337.954 kb on - strand at 3338.024 kb on - strand, within Dshi_3175 at 3338.047 kb on + strand, within Dshi_3175 at 3338.058 kb on + strand, within Dshi_3175 at 3338.071 kb on - strand, within Dshi_3175 at 3338.140 kb on + strand, within Dshi_3175 at 3338.140 kb on + strand, within Dshi_3175 at 3338.148 kb on - strand, within Dshi_3175 at 3338.163 kb on + strand, within Dshi_3175 at 3338.163 kb on + strand, within Dshi_3175 at 3338.171 kb on - strand, within Dshi_3175 at 3338.171 kb on - strand, within Dshi_3175 at 3338.171 kb on - strand, within Dshi_3175 at 3338.171 kb on - strand, within Dshi_3175 at 3338.210 kb on + strand, within Dshi_3175 at 3338.231 kb on - strand, within Dshi_3175 at 3338.341 kb on + strand, within Dshi_3175 at 3338.346 kb on + strand, within Dshi_3175 at 3338.435 kb on - strand at 3338.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 3,335,286 + Dshi_3172 0.35 +3.8 3,335,335 + Dshi_3172 0.38 +0.3 3,335,343 - Dshi_3172 0.39 -1.7 3,335,343 - Dshi_3172 0.39 +0.6 3,335,379 - Dshi_3172 0.41 -0.7 3,335,379 - Dshi_3172 0.41 -0.4 3,335,412 - Dshi_3172 0.43 +2.6 3,335,615 + Dshi_3172 0.56 +0.6 3,335,616 + Dshi_3172 0.56 +1.4 3,335,623 - Dshi_3172 0.57 -2.4 3,335,624 - Dshi_3172 0.57 -1.0 3,335,689 - Dshi_3172 0.61 -1.1 3,335,708 - Dshi_3172 0.62 -1.7 3,335,836 + Dshi_3172 0.70 -3.4 3,335,836 + Dshi_3172 0.70 -0.6 3,335,836 + Dshi_3172 0.70 +1.2 3,335,844 - Dshi_3172 0.71 -0.0 3,335,903 + Dshi_3172 0.75 +0.2 3,335,916 + Dshi_3172 0.76 -0.0 3,335,916 + Dshi_3172 0.76 -0.2 3,336,012 + Dshi_3172 0.82 +1.6 3,336,015 - Dshi_3172 0.82 -1.6 3,336,100 - Dshi_3172 0.87 +0.1 3,336,118 - Dshi_3172 0.89 -0.3 3,336,123 - Dshi_3172 0.89 -0.9 3,336,132 + Dshi_3172 0.89 +3.8 3,336,160 + +0.4 3,336,162 - +1.6 3,336,364 + -0.3 3,336,381 + -1.1 3,336,388 + +0.5 3,336,415 - Dshi_3173 0.12 +0.4 3,336,509 + Dshi_3173 0.20 -2.0 3,336,534 - Dshi_3173 0.22 +2.9 3,336,551 + Dshi_3173 0.23 -0.5 3,336,567 + Dshi_3173 0.24 -1.0 3,336,586 + Dshi_3173 0.26 +0.6 3,336,672 + Dshi_3173 0.33 -2.2 3,336,767 + Dshi_3173 0.41 +0.4 3,336,771 + Dshi_3173 0.41 +0.0 3,336,780 - Dshi_3173 0.42 +1.2 3,336,842 - Dshi_3173 0.47 -0.5 3,336,877 + Dshi_3173 0.50 -2.4 3,336,877 + Dshi_3173 0.50 +1.1 3,336,877 + Dshi_3173 0.50 +0.0 3,336,882 + Dshi_3173 0.50 +0.4 3,336,904 - Dshi_3173 0.52 -0.5 3,336,930 - Dshi_3173 0.54 -1.7 3,336,952 + Dshi_3173 0.56 +0.6 3,336,983 + Dshi_3173 0.59 -1.5 3,336,988 + Dshi_3173 0.59 -1.4 3,337,080 + Dshi_3173 0.67 -0.1 3,337,144 - Dshi_3173 0.72 +1.6 3,337,193 - Dshi_3173 0.76 -0.2 3,337,249 + Dshi_3173 0.81 +2.1 3,337,253 + Dshi_3173 0.81 +1.6 3,337,257 - Dshi_3173 0.81 -2.0 3,337,267 + Dshi_3173 0.82 -3.1 3,337,276 - Dshi_3173 0.83 +2.3 3,337,310 - Dshi_3173 0.86 -0.5 3,337,341 + Dshi_3173 0.88 -1.1 3,337,381 - +0.6 3,337,398 + +0.6 3,337,409 + -0.8 3,337,417 - +0.6 3,337,447 - -0.1 3,337,465 - -2.7 3,337,465 - +0.2 3,337,474 + +1.0 3,337,517 - +1.6 3,337,535 + Dshi_3174 0.10 +1.4 3,337,606 - Dshi_3174 0.25 +1.6 3,337,662 - Dshi_3174 0.36 -2.2 3,337,739 - Dshi_3174 0.52 -0.5 3,337,748 + Dshi_3174 0.54 +0.4 3,337,754 - Dshi_3174 0.55 +0.3 3,337,754 - Dshi_3174 0.55 -1.0 3,337,765 - Dshi_3174 0.57 -2.2 3,337,790 - Dshi_3174 0.63 +0.6 3,337,830 + Dshi_3174 0.71 +0.9 3,337,830 + Dshi_3174 0.71 -2.2 3,337,838 - Dshi_3174 0.72 +1.9 3,337,838 - Dshi_3174 0.72 +0.5 3,337,838 - Dshi_3174 0.72 -0.7 3,337,907 + Dshi_3174 0.87 -1.8 3,337,929 + +1.1 3,337,954 - -0.4 3,338,024 - Dshi_3175 0.12 +0.4 3,338,047 + Dshi_3175 0.18 -0.6 3,338,058 + Dshi_3175 0.20 -0.4 3,338,071 - Dshi_3175 0.23 +2.2 3,338,140 + Dshi_3175 0.38 -0.7 3,338,140 + Dshi_3175 0.38 +2.9 3,338,148 - Dshi_3175 0.40 +1.6 3,338,163 + Dshi_3175 0.43 +0.6 3,338,163 + Dshi_3175 0.43 +0.3 3,338,171 - Dshi_3175 0.45 -1.8 3,338,171 - Dshi_3175 0.45 -0.8 3,338,171 - Dshi_3175 0.45 -0.6 3,338,171 - Dshi_3175 0.45 -1.5 3,338,210 + Dshi_3175 0.54 -1.5 3,338,231 - Dshi_3175 0.58 -0.7 3,338,341 + Dshi_3175 0.83 +1.2 3,338,346 + Dshi_3175 0.84 -1.5 3,338,435 - -0.3 3,338,458 + +0.6
Or see this region's nucleotide sequence