Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2970 and Dshi_2971 are separated by 133 nucleotides Dshi_2971 and Dshi_2972 overlap by 28 nucleotides Dshi_2972 and Dshi_2973 are separated by 95 nucleotides
Dshi_2970: Dshi_2970 - D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding (RefSeq), at 3,136,810 to 3,137,796
_2970
Dshi_2971: Dshi_2971 - protein of unknown function DUF1058 (RefSeq), at 3,137,930 to 3,138,544
_2971
Dshi_2972: Dshi_2972 - putative PAS/PAC sensor protein (RefSeq), at 3,138,517 to 3,139,428
_2972
Dshi_2973: Dshi_2973 - Peptidyl-dipeptidase Dcp (RefSeq), at 3,139,524 to 3,141,545
_2973
Position (kb)
3137
3138
3139 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3136.956 kb on - strand, within Dshi_2970 at 3136.970 kb on - strand, within Dshi_2970 at 3137.062 kb on - strand, within Dshi_2970 at 3137.459 kb on + strand, within Dshi_2970 at 3137.490 kb on - strand, within Dshi_2970 at 3137.498 kb on - strand, within Dshi_2970 at 3137.498 kb on - strand, within Dshi_2970 at 3137.547 kb on + strand, within Dshi_2970 at 3137.577 kb on + strand, within Dshi_2970 at 3137.735 kb on - strand at 3137.765 kb on - strand at 3137.769 kb on + strand at 3137.776 kb on + strand at 3137.812 kb on - strand at 3137.812 kb on - strand at 3137.812 kb on - strand at 3137.812 kb on - strand at 3137.842 kb on - strand at 3137.925 kb on - strand at 3138.486 kb on + strand at 3138.496 kb on + strand at 3138.496 kb on + strand at 3138.496 kb on + strand at 3138.517 kb on - strand at 3138.527 kb on - strand at 3138.547 kb on - strand at 3138.560 kb on - strand at 3138.646 kb on + strand, within Dshi_2972 at 3138.911 kb on + strand, within Dshi_2972 at 3138.930 kb on + strand, within Dshi_2972 at 3138.953 kb on + strand, within Dshi_2972 at 3138.961 kb on - strand, within Dshi_2972 at 3139.034 kb on - strand, within Dshi_2972 at 3139.105 kb on - strand, within Dshi_2972 at 3139.124 kb on - strand, within Dshi_2972 at 3139.147 kb on + strand, within Dshi_2972 at 3139.249 kb on + strand, within Dshi_2972 at 3139.282 kb on + strand, within Dshi_2972 at 3139.397 kb on - strand at 3139.431 kb on - strand at 3139.497 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 3,136,956 - Dshi_2970 0.15 +1.1 3,136,970 - Dshi_2970 0.16 +3.0 3,137,062 - Dshi_2970 0.26 -3.7 3,137,459 + Dshi_2970 0.66 -1.7 3,137,490 - Dshi_2970 0.69 -0.9 3,137,498 - Dshi_2970 0.70 -1.7 3,137,498 - Dshi_2970 0.70 -1.2 3,137,547 + Dshi_2970 0.75 -0.4 3,137,577 + Dshi_2970 0.78 -0.7 3,137,735 - -1.9 3,137,765 - +0.2 3,137,769 + -3.3 3,137,776 + -1.5 3,137,812 - -0.8 3,137,812 - -3.5 3,137,812 - -2.2 3,137,812 - -0.2 3,137,842 - -1.2 3,137,925 - -0.1 3,138,486 + +0.0 3,138,496 + +0.1 3,138,496 + +0.4 3,138,496 + +1.0 3,138,517 - -0.3 3,138,527 - -0.6 3,138,547 - -1.7 3,138,560 - +1.7 3,138,646 + Dshi_2972 0.14 -1.1 3,138,911 + Dshi_2972 0.43 +1.3 3,138,930 + Dshi_2972 0.45 +1.8 3,138,953 + Dshi_2972 0.48 +0.1 3,138,961 - Dshi_2972 0.49 +0.7 3,139,034 - Dshi_2972 0.57 -1.0 3,139,105 - Dshi_2972 0.64 -3.7 3,139,124 - Dshi_2972 0.67 -2.8 3,139,147 + Dshi_2972 0.69 -0.9 3,139,249 + Dshi_2972 0.80 +1.0 3,139,282 + Dshi_2972 0.84 +1.1 3,139,397 - -0.9 3,139,431 - -2.7 3,139,497 - +0.7
Or see this region's nucleotide sequence