Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2442 and Dshi_2443 are separated by 51 nucleotides Dshi_2443 and Dshi_2444 overlap by 4 nucleotides
Dshi_2442: Dshi_2442 - aldehyde dehydrogenase (RefSeq), at 2,584,873 to 2,586,378
_2442
Dshi_2443: Dshi_2443 - 6-phosphogluconate dehydrogenase NAD-binding (RefSeq), at 2,586,430 to 2,587,299
_2443
Dshi_2444: Dshi_2444 - 4-hydroxythreonine-4-phosphate dehydrogenase (RefSeq), at 2,587,296 to 2,588,300
_2444
Position (kb)
2586
2587
2588 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2585.527 kb on + strand, within Dshi_2442 at 2585.740 kb on + strand, within Dshi_2442 at 2585.820 kb on + strand, within Dshi_2442 at 2585.934 kb on + strand, within Dshi_2442 at 2586.091 kb on - strand, within Dshi_2442 at 2586.094 kb on + strand, within Dshi_2442 at 2586.103 kb on - strand, within Dshi_2442 at 2586.244 kb on + strand at 2586.256 kb on - strand at 2586.324 kb on - strand at 2586.427 kb on + strand at 2586.535 kb on + strand, within Dshi_2443 at 2586.543 kb on - strand, within Dshi_2443 at 2586.543 kb on - strand, within Dshi_2443 at 2586.579 kb on - strand, within Dshi_2443 at 2586.611 kb on + strand, within Dshi_2443 at 2586.710 kb on + strand, within Dshi_2443 at 2586.750 kb on + strand, within Dshi_2443 at 2586.758 kb on - strand, within Dshi_2443 at 2586.763 kb on - strand, within Dshi_2443 at 2586.894 kb on - strand, within Dshi_2443 at 2586.992 kb on + strand, within Dshi_2443 at 2586.995 kb on - strand, within Dshi_2443 at 2586.998 kb on + strand, within Dshi_2443 at 2587.033 kb on - strand, within Dshi_2443 at 2587.091 kb on - strand, within Dshi_2443 at 2587.330 kb on + strand at 2587.453 kb on + strand, within Dshi_2444 at 2587.648 kb on + strand, within Dshi_2444 at 2587.650 kb on - strand, within Dshi_2444 at 2587.656 kb on + strand, within Dshi_2444 at 2587.827 kb on - strand, within Dshi_2444 at 2587.873 kb on - strand, within Dshi_2444 at 2588.002 kb on - strand, within Dshi_2444 at 2588.085 kb on + strand, within Dshi_2444 at 2588.125 kb on - strand, within Dshi_2444 at 2588.125 kb on - strand, within Dshi_2444 at 2588.259 kb on + strand at 2588.259 kb on - strand at 2588.260 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 2,585,527 + Dshi_2442 0.43 +0.9 2,585,740 + Dshi_2442 0.58 -0.7 2,585,820 + Dshi_2442 0.63 +1.3 2,585,934 + Dshi_2442 0.70 -1.3 2,586,091 - Dshi_2442 0.81 +1.6 2,586,094 + Dshi_2442 0.81 +0.3 2,586,103 - Dshi_2442 0.82 +0.3 2,586,244 + +0.3 2,586,256 - +0.3 2,586,324 - +1.0 2,586,427 + -1.5 2,586,535 + Dshi_2443 0.12 -0.6 2,586,543 - Dshi_2443 0.13 -3.6 2,586,543 - Dshi_2443 0.13 -3.0 2,586,579 - Dshi_2443 0.17 -1.6 2,586,611 + Dshi_2443 0.21 +0.0 2,586,710 + Dshi_2443 0.32 +0.0 2,586,750 + Dshi_2443 0.37 +1.3 2,586,758 - Dshi_2443 0.38 +1.9 2,586,763 - Dshi_2443 0.38 -2.3 2,586,894 - Dshi_2443 0.53 -2.9 2,586,992 + Dshi_2443 0.65 +0.7 2,586,995 - Dshi_2443 0.65 -1.0 2,586,998 + Dshi_2443 0.65 -0.1 2,587,033 - Dshi_2443 0.69 -2.0 2,587,091 - Dshi_2443 0.76 -0.7 2,587,330 + +4.1 2,587,453 + Dshi_2444 0.16 -2.7 2,587,648 + Dshi_2444 0.35 -2.2 2,587,650 - Dshi_2444 0.35 +0.3 2,587,656 + Dshi_2444 0.36 +1.9 2,587,827 - Dshi_2444 0.53 -0.7 2,587,873 - Dshi_2444 0.57 +0.3 2,588,002 - Dshi_2444 0.70 +1.3 2,588,085 + Dshi_2444 0.79 +0.0 2,588,125 - Dshi_2444 0.82 -3.3 2,588,125 - Dshi_2444 0.82 +0.1 2,588,259 + +0.3 2,588,259 - +0.9 2,588,260 + -0.4
Or see this region's nucleotide sequence