Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2364 and Dshi_2365 are separated by 757 nucleotides Dshi_2365 and Dshi_2366 are separated by 135 nucleotides Dshi_2366 and Dshi_2367 are separated by 112 nucleotides
Dshi_2364: Dshi_2364 - hypothetical protein (RefSeq), at 2,497,676 to 2,497,882
_2364
Dshi_2365: Dshi_2365 - urease, alpha subunit (RefSeq), at 2,498,640 to 2,500,349
_2365
Dshi_2366: Dshi_2366 - hypothetical protein (RefSeq), at 2,500,485 to 2,500,814
_2366
Dshi_2367: Dshi_2367 - alginate regulatory protein AlgP (RefSeq), at 2,500,927 to 2,501,649
_2367
Position (kb)
2498
2499
2500
2501 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2497.728 kb on + strand, within Dshi_2364 at 2497.745 kb on + strand, within Dshi_2364 at 2497.799 kb on - strand, within Dshi_2364 at 2497.915 kb on - strand at 2497.954 kb on + strand at 2498.121 kb on - strand at 2498.166 kb on - strand at 2498.195 kb on - strand at 2498.195 kb on - strand at 2498.385 kb on - strand at 2498.401 kb on - strand at 2498.489 kb on - strand at 2498.549 kb on + strand at 2498.567 kb on - strand at 2498.595 kb on - strand at 2498.600 kb on - strand at 2498.610 kb on - strand at 2498.658 kb on - strand at 2498.699 kb on - strand at 2498.717 kb on + strand at 2498.760 kb on + strand at 2498.789 kb on - strand at 2498.804 kb on - strand at 2498.833 kb on - strand, within Dshi_2365 at 2498.839 kb on - strand, within Dshi_2365 at 2498.926 kb on + strand, within Dshi_2365 at 2498.933 kb on - strand, within Dshi_2365 at 2498.989 kb on + strand, within Dshi_2365 at 2499.003 kb on - strand, within Dshi_2365 at 2499.015 kb on - strand, within Dshi_2365 at 2499.076 kb on + strand, within Dshi_2365 at 2499.236 kb on - strand, within Dshi_2365 at 2499.249 kb on + strand, within Dshi_2365 at 2499.249 kb on + strand, within Dshi_2365 at 2499.257 kb on - strand, within Dshi_2365 at 2499.257 kb on - strand, within Dshi_2365 at 2499.276 kb on + strand, within Dshi_2365 at 2499.420 kb on + strand, within Dshi_2365 at 2499.421 kb on - strand, within Dshi_2365 at 2499.435 kb on + strand, within Dshi_2365 at 2499.438 kb on - strand, within Dshi_2365 at 2499.459 kb on + strand, within Dshi_2365 at 2499.520 kb on + strand, within Dshi_2365 at 2499.552 kb on - strand, within Dshi_2365 at 2499.557 kb on - strand, within Dshi_2365 at 2499.598 kb on - strand, within Dshi_2365 at 2499.607 kb on - strand, within Dshi_2365 at 2499.676 kb on + strand, within Dshi_2365 at 2499.920 kb on + strand, within Dshi_2365 at 2500.082 kb on + strand, within Dshi_2365 at 2500.082 kb on + strand, within Dshi_2365 at 2500.096 kb on - strand, within Dshi_2365 at 2500.168 kb on + strand, within Dshi_2365 at 2500.176 kb on - strand, within Dshi_2365 at 2500.178 kb on + strand, within Dshi_2365 at 2500.306 kb on + strand at 2500.345 kb on + strand at 2500.439 kb on + strand at 2500.491 kb on - strand at 2500.540 kb on - strand, within Dshi_2366 at 2500.636 kb on + strand, within Dshi_2366 at 2500.636 kb on + strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.644 kb on - strand, within Dshi_2366 at 2500.679 kb on - strand, within Dshi_2366 at 2500.747 kb on + strand, within Dshi_2366 at 2500.750 kb on - strand, within Dshi_2366 at 2500.789 kb on + strand at 2500.791 kb on - strand at 2500.791 kb on - strand at 2500.797 kb on - strand at 2500.802 kb on - strand at 2500.802 kb on - strand at 2500.817 kb on - strand at 2500.843 kb on + strand at 2500.908 kb on + strand at 2500.913 kb on + strand at 2500.970 kb on - strand at 2500.979 kb on + strand at 2500.979 kb on + strand at 2500.987 kb on - strand at 2501.033 kb on - strand, within Dshi_2367 at 2501.145 kb on - strand, within Dshi_2367 at 2501.296 kb on - strand, within Dshi_2367 at 2501.306 kb on + strand, within Dshi_2367 at 2501.307 kb on - strand, within Dshi_2367
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 2,497,728 + Dshi_2364 0.25 +0.7 2,497,745 + Dshi_2364 0.33 -1.3 2,497,799 - Dshi_2364 0.59 +2.8 2,497,915 - +0.7 2,497,954 + -1.7 2,498,121 - +1.8 2,498,166 - -0.6 2,498,195 - -0.3 2,498,195 - -1.3 2,498,385 - -0.9 2,498,401 - -2.0 2,498,489 - +2.6 2,498,549 + +1.5 2,498,567 - -2.1 2,498,595 - +0.3 2,498,600 - -2.5 2,498,610 - -0.7 2,498,658 - -3.6 2,498,699 - -1.2 2,498,717 + +1.4 2,498,760 + -0.0 2,498,789 - -1.3 2,498,804 - +0.3 2,498,833 - Dshi_2365 0.11 -1.0 2,498,839 - Dshi_2365 0.12 +0.3 2,498,926 + Dshi_2365 0.17 -2.2 2,498,933 - Dshi_2365 0.17 -0.7 2,498,989 + Dshi_2365 0.20 -0.3 2,499,003 - Dshi_2365 0.21 +0.1 2,499,015 - Dshi_2365 0.22 +0.7 2,499,076 + Dshi_2365 0.25 -0.1 2,499,236 - Dshi_2365 0.35 -0.0 2,499,249 + Dshi_2365 0.36 -1.4 2,499,249 + Dshi_2365 0.36 -0.6 2,499,257 - Dshi_2365 0.36 -1.6 2,499,257 - Dshi_2365 0.36 +1.3 2,499,276 + Dshi_2365 0.37 -0.9 2,499,420 + Dshi_2365 0.46 +1.3 2,499,421 - Dshi_2365 0.46 -0.7 2,499,435 + Dshi_2365 0.46 -1.1 2,499,438 - Dshi_2365 0.47 -0.8 2,499,459 + Dshi_2365 0.48 -0.6 2,499,520 + Dshi_2365 0.51 +0.8 2,499,552 - Dshi_2365 0.53 +0.8 2,499,557 - Dshi_2365 0.54 +3.7 2,499,598 - Dshi_2365 0.56 -1.0 2,499,607 - Dshi_2365 0.57 +1.8 2,499,676 + Dshi_2365 0.61 -2.3 2,499,920 + Dshi_2365 0.75 -0.3 2,500,082 + Dshi_2365 0.84 -0.4 2,500,082 + Dshi_2365 0.84 -0.5 2,500,096 - Dshi_2365 0.85 -0.8 2,500,168 + Dshi_2365 0.89 -0.0 2,500,176 - Dshi_2365 0.90 -1.9 2,500,178 + Dshi_2365 0.90 -0.5 2,500,306 + -0.1 2,500,345 + +1.2 2,500,439 + +0.2 2,500,491 - +2.2 2,500,540 - Dshi_2366 0.17 -0.2 2,500,636 + Dshi_2366 0.46 +2.6 2,500,636 + Dshi_2366 0.46 -1.2 2,500,644 - Dshi_2366 0.48 -0.8 2,500,644 - Dshi_2366 0.48 -1.3 2,500,644 - Dshi_2366 0.48 -3.3 2,500,679 - Dshi_2366 0.59 -1.9 2,500,747 + Dshi_2366 0.79 +0.2 2,500,750 - Dshi_2366 0.80 -1.7 2,500,789 + +1.2 2,500,791 - -1.5 2,500,791 - -4.1 2,500,797 - -0.6 2,500,802 - -0.2 2,500,802 - +1.2 2,500,817 - -1.1 2,500,843 + +2.6 2,500,908 + -2.6 2,500,913 + -1.6 2,500,970 - -2.8 2,500,979 + -0.8 2,500,979 + +1.0 2,500,987 - -1.1 2,501,033 - Dshi_2367 0.15 -0.5 2,501,145 - Dshi_2367 0.30 +0.2 2,501,296 - Dshi_2367 0.51 -0.2 2,501,306 + Dshi_2367 0.52 -1.5 2,501,307 - Dshi_2367 0.53 +0.8
Or see this region's nucleotide sequence