Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1987 and Dshi_1988 are separated by 99 nucleotides Dshi_1988 and Dshi_1989 are separated by 3 nucleotides Dshi_1989 and Dshi_R0040 are separated by 127 nucleotides Dshi_R0040 and Dshi_1990 are separated by 95 nucleotides
Dshi_1987: Dshi_1987 - hypothetical protein (RefSeq), at 2,102,173 to 2,102,721
_1987
Dshi_1988: Dshi_1988 - hypothetical protein (RefSeq), at 2,102,821 to 2,103,216
_1988
Dshi_1989: Dshi_1989 - secretion protein HlyD family protein (RefSeq), at 2,103,220 to 2,104,452
_1989
Dshi_R0040: Dshi_R0040 - tRNA-Arg (RefSeq), at 2,104,580 to 2,104,656
_R0040
Dshi_1990: Dshi_1990 - aminoglycoside phosphotransferase (RefSeq), at 2,104,752 to 2,106,995
_1990
Position (kb)
2103
2104
2105 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2102.258 kb on + strand, within Dshi_1987 at 2102.258 kb on + strand, within Dshi_1987 at 2102.266 kb on - strand, within Dshi_1987 at 2102.278 kb on + strand, within Dshi_1987 at 2102.286 kb on - strand, within Dshi_1987 at 2102.286 kb on - strand, within Dshi_1987 at 2102.466 kb on - strand, within Dshi_1987 at 2102.652 kb on + strand, within Dshi_1987 at 2102.868 kb on + strand, within Dshi_1988 at 2102.947 kb on - strand, within Dshi_1988 at 2102.968 kb on + strand, within Dshi_1988 at 2103.008 kb on - strand, within Dshi_1988 at 2103.200 kb on - strand at 2103.261 kb on - strand at 2103.296 kb on - strand at 2103.355 kb on - strand, within Dshi_1989 at 2103.371 kb on + strand, within Dshi_1989 at 2103.379 kb on - strand, within Dshi_1989 at 2103.421 kb on - strand at 2103.822 kb on - strand, within Dshi_1989 at 2103.887 kb on - strand, within Dshi_1989 at 2104.063 kb on + strand, within Dshi_1989 at 2104.164 kb on - strand, within Dshi_1989 at 2104.164 kb on - strand, within Dshi_1989 at 2104.230 kb on - strand, within Dshi_1989 at 2104.258 kb on - strand, within Dshi_1989 at 2104.322 kb on - strand, within Dshi_1989 at 2104.824 kb on + strand at 2104.849 kb on + strand at 2104.879 kb on - strand at 2105.007 kb on + strand, within Dshi_1990 at 2105.048 kb on + strand, within Dshi_1990 at 2105.172 kb on - strand at 2105.172 kb on - strand, within Dshi_1990 at 2105.294 kb on - strand, within Dshi_1990 at 2105.407 kb on + strand, within Dshi_1990
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 2,102,258 + Dshi_1987 0.15 -1.6 2,102,258 + Dshi_1987 0.15 +0.4 2,102,266 - Dshi_1987 0.17 +2.0 2,102,278 + Dshi_1987 0.19 -0.5 2,102,286 - Dshi_1987 0.21 -0.3 2,102,286 - Dshi_1987 0.21 -0.5 2,102,466 - Dshi_1987 0.53 +0.4 2,102,652 + Dshi_1987 0.87 +1.4 2,102,868 + Dshi_1988 0.12 +1.7 2,102,947 - Dshi_1988 0.32 -1.2 2,102,968 + Dshi_1988 0.37 +2.0 2,103,008 - Dshi_1988 0.47 -1.2 2,103,200 - -1.0 2,103,261 - -3.5 2,103,296 - +0.4 2,103,355 - Dshi_1989 0.11 +0.4 2,103,371 + Dshi_1989 0.12 -1.8 2,103,379 - Dshi_1989 0.13 +0.4 2,103,421 - +0.4 2,103,822 - Dshi_1989 0.49 -0.4 2,103,887 - Dshi_1989 0.54 +0.6 2,104,063 + Dshi_1989 0.68 -3.1 2,104,164 - Dshi_1989 0.77 -1.6 2,104,164 - Dshi_1989 0.77 -0.9 2,104,230 - Dshi_1989 0.82 -2.6 2,104,258 - Dshi_1989 0.84 +0.4 2,104,322 - Dshi_1989 0.89 +0.4 2,104,824 + -2.3 2,104,849 + -3.3 2,104,879 - -1.2 2,105,007 + Dshi_1990 0.11 -0.6 2,105,048 + Dshi_1990 0.13 +1.0 2,105,172 - +0.4 2,105,172 - Dshi_1990 0.19 -2.2 2,105,294 - Dshi_1990 0.24 -0.2 2,105,407 + Dshi_1990 0.29 -1.9
Or see this region's nucleotide sequence