Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1685 and Dshi_1686 are separated by 10 nucleotides Dshi_1686 and Dshi_1687 are separated by 127 nucleotides Dshi_1687 and Dshi_1688 are separated by 189 nucleotides Dshi_1688 and Dshi_1689 overlap by 4 nucleotides
Dshi_1685: Dshi_1685 - Radical SAM domain protein (RefSeq), at 1,754,813 to 1,755,757
_1685
Dshi_1686: Dshi_1686 - transglutaminase domain protein (RefSeq), at 1,755,768 to 1,756,544
_1686
Dshi_1687: Dshi_1687 - HNH endonuclease (RefSeq), at 1,756,672 to 1,757,268
_1687
Dshi_1688: Dshi_1688 - phospholipase/Carboxylesterase (RefSeq), at 1,757,458 to 1,758,129
_1688
Dshi_1689: Dshi_1689 - HhH-GPD family protein (RefSeq), at 1,758,126 to 1,758,758
_1689
Position (kb)
1756
1757
1758 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1755.728 kb on + strand at 1755.856 kb on + strand, within Dshi_1686 at 1755.925 kb on + strand, within Dshi_1686 at 1756.017 kb on - strand, within Dshi_1686 at 1756.161 kb on - strand, within Dshi_1686 at 1756.182 kb on - strand, within Dshi_1686 at 1756.323 kb on - strand, within Dshi_1686 at 1756.323 kb on - strand, within Dshi_1686 at 1756.334 kb on + strand, within Dshi_1686 at 1756.352 kb on - strand, within Dshi_1686 at 1756.492 kb on + strand at 1756.539 kb on - strand at 1756.723 kb on - strand at 1756.791 kb on + strand, within Dshi_1687 at 1756.795 kb on - strand, within Dshi_1687 at 1756.805 kb on + strand, within Dshi_1687 at 1756.811 kb on - strand, within Dshi_1687 at 1756.895 kb on - strand, within Dshi_1687 at 1756.897 kb on + strand, within Dshi_1687 at 1756.926 kb on - strand, within Dshi_1687 at 1757.007 kb on - strand, within Dshi_1687 at 1757.071 kb on - strand, within Dshi_1687 at 1757.129 kb on - strand, within Dshi_1687 at 1757.360 kb on + strand at 1758.111 kb on + strand at 1758.227 kb on + strand, within Dshi_1689
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 1,755,728 + +3.3 1,755,856 + Dshi_1686 0.11 -0.6 1,755,925 + Dshi_1686 0.20 -1.1 1,756,017 - Dshi_1686 0.32 -0.1 1,756,161 - Dshi_1686 0.51 +1.1 1,756,182 - Dshi_1686 0.53 +1.3 1,756,323 - Dshi_1686 0.71 -1.2 1,756,323 - Dshi_1686 0.71 -0.8 1,756,334 + Dshi_1686 0.73 -2.0 1,756,352 - Dshi_1686 0.75 -0.3 1,756,492 + +0.1 1,756,539 - +1.2 1,756,723 - -0.4 1,756,791 + Dshi_1687 0.20 +0.3 1,756,795 - Dshi_1687 0.21 +0.6 1,756,805 + Dshi_1687 0.22 +1.6 1,756,811 - Dshi_1687 0.23 -2.1 1,756,895 - Dshi_1687 0.37 -1.1 1,756,897 + Dshi_1687 0.38 +0.3 1,756,926 - Dshi_1687 0.43 +0.2 1,757,007 - Dshi_1687 0.56 -0.6 1,757,071 - Dshi_1687 0.67 -1.3 1,757,129 - Dshi_1687 0.77 +0.0 1,757,360 + +0.7 1,758,111 + +1.3 1,758,227 + Dshi_1689 0.16 -1.3
Or see this region's nucleotide sequence