Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1682 and Dshi_1683 are separated by 24 nucleotides Dshi_1683 and Dshi_1684 are separated by 11 nucleotides Dshi_1684 and Dshi_1685 are separated by 242 nucleotides
Dshi_1682: Dshi_1682 - Glucose-6-phosphate isomerase (RefSeq), at 1,750,801 to 1,752,405
_1682
Dshi_1683: Dshi_1683 - 6-phosphogluconolactonase (RefSeq), at 1,752,430 to 1,753,104
_1683
Dshi_1684: Dshi_1684 - glucose-6-phosphate 1-dehydrogenase (RefSeq), at 1,753,116 to 1,754,570
_1684
Dshi_1685: Dshi_1685 - Radical SAM domain protein (RefSeq), at 1,754,813 to 1,755,757
_1685
Position (kb)
1753
1754
1755 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1752.523 kb on + strand, within Dshi_1683 at 1752.523 kb on + strand, within Dshi_1683 at 1752.573 kb on + strand, within Dshi_1683 at 1752.687 kb on + strand, within Dshi_1683 at 1752.866 kb on - strand, within Dshi_1683 at 1754.586 kb on - strand at 1754.587 kb on + strand at 1754.629 kb on + strand at 1754.723 kb on + strand at 1754.760 kb on - strand at 1754.878 kb on - strand at 1754.930 kb on - strand, within Dshi_1685 at 1754.986 kb on - strand, within Dshi_1685 at 1754.992 kb on + strand, within Dshi_1685 at 1755.001 kb on - strand, within Dshi_1685 at 1755.001 kb on - strand, within Dshi_1685 at 1755.054 kb on - strand, within Dshi_1685 at 1755.115 kb on + strand, within Dshi_1685 at 1755.138 kb on + strand, within Dshi_1685 at 1755.255 kb on - strand, within Dshi_1685 at 1755.421 kb on + strand, within Dshi_1685 at 1755.461 kb on - strand, within Dshi_1685 at 1755.498 kb on + strand, within Dshi_1685
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 1,752,523 + Dshi_1683 0.14 +0.6 1,752,523 + Dshi_1683 0.14 +1.6 1,752,573 + Dshi_1683 0.21 -2.1 1,752,687 + Dshi_1683 0.38 +2.3 1,752,866 - Dshi_1683 0.65 -3.7 1,754,586 - -0.1 1,754,587 + -0.5 1,754,629 + -0.9 1,754,723 + -1.1 1,754,760 - -1.3 1,754,878 - +0.0 1,754,930 - Dshi_1685 0.12 +1.4 1,754,986 - Dshi_1685 0.18 -0.7 1,754,992 + Dshi_1685 0.19 -0.6 1,755,001 - Dshi_1685 0.20 -1.0 1,755,001 - Dshi_1685 0.20 +1.0 1,755,054 - Dshi_1685 0.26 -0.6 1,755,115 + Dshi_1685 0.32 +0.6 1,755,138 + Dshi_1685 0.34 +0.3 1,755,255 - Dshi_1685 0.47 +2.0 1,755,421 + Dshi_1685 0.64 -1.8 1,755,461 - Dshi_1685 0.69 -0.1 1,755,498 + Dshi_1685 0.72 -0.2
Or see this region's nucleotide sequence