Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1061 and Dshi_1062 are separated by 59 nucleotides Dshi_1062 and Dshi_1063 are separated by 79 nucleotides Dshi_1063 and Dshi_1064 overlap by 8 nucleotides Dshi_1064 and Dshi_1065 are separated by 379 nucleotides Dshi_1065 and Dshi_1066 are separated by 81 nucleotides
Dshi_1061: Dshi_1061 - hypothetical protein (RefSeq), at 1,095,405 to 1,096,250
_1061
Dshi_1062: Dshi_1062 - Isoprenylcysteine carboxyl methyltransferase (RefSeq), at 1,096,310 to 1,096,927
_1062
Dshi_1063: Dshi_1063 - protein of unknown function DUF1289 (RefSeq), at 1,097,007 to 1,097,243
_1063
Dshi_1064: Dshi_1064 - Holliday junction resolvase YqgF (RefSeq), at 1,097,236 to 1,097,727
_1064
Dshi_1065: Dshi_1065 - hypothetical protein (RefSeq), at 1,098,107 to 1,098,376
_1065
Dshi_1066: Dshi_1066 - hypothetical protein (RefSeq), at 1,098,458 to 1,098,886
_1066
Position (kb)
1097
1098 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1096.316 kb on - strand at 1096.343 kb on - strand at 1096.437 kb on - strand, within Dshi_1062 at 1096.478 kb on + strand, within Dshi_1062 at 1096.599 kb on + strand, within Dshi_1062 at 1096.607 kb on - strand, within Dshi_1062 at 1096.658 kb on + strand, within Dshi_1062 at 1096.745 kb on - strand, within Dshi_1062 at 1096.780 kb on - strand, within Dshi_1062 at 1096.848 kb on - strand, within Dshi_1062 at 1096.886 kb on - strand at 1096.936 kb on - strand at 1097.072 kb on + strand, within Dshi_1063 at 1097.072 kb on + strand, within Dshi_1063 at 1097.082 kb on + strand, within Dshi_1063 at 1097.090 kb on - strand, within Dshi_1063 at 1097.190 kb on + strand, within Dshi_1063 at 1097.193 kb on - strand, within Dshi_1063 at 1097.231 kb on + strand at 1097.231 kb on - strand at 1097.822 kb on - strand at 1097.896 kb on + strand at 1097.913 kb on - strand at 1098.012 kb on + strand at 1098.014 kb on + strand at 1098.091 kb on + strand at 1098.096 kb on - strand at 1098.098 kb on - strand at 1098.123 kb on + strand at 1098.133 kb on + strand at 1098.141 kb on - strand, within Dshi_1065 at 1098.194 kb on + strand, within Dshi_1065 at 1098.247 kb on - strand, within Dshi_1065 at 1098.350 kb on + strand at 1098.500 kb on + strand at 1098.540 kb on + strand, within Dshi_1066 at 1098.548 kb on - strand, within Dshi_1066 at 1098.563 kb on - strand, within Dshi_1066 at 1098.563 kb on - strand, within Dshi_1066 at 1098.605 kb on - strand, within Dshi_1066 at 1098.656 kb on + strand, within Dshi_1066
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 1,096,316 - -1.4 1,096,343 - +2.7 1,096,437 - Dshi_1062 0.21 +2.4 1,096,478 + Dshi_1062 0.27 -0.5 1,096,599 + Dshi_1062 0.47 +2.4 1,096,607 - Dshi_1062 0.48 +3.2 1,096,658 + Dshi_1062 0.56 +2.4 1,096,745 - Dshi_1062 0.70 -2.2 1,096,780 - Dshi_1062 0.76 +0.4 1,096,848 - Dshi_1062 0.87 +0.5 1,096,886 - +0.7 1,096,936 - +2.0 1,097,072 + Dshi_1063 0.27 -0.6 1,097,072 + Dshi_1063 0.27 +0.4 1,097,082 + Dshi_1063 0.32 -2.9 1,097,090 - Dshi_1063 0.35 -1.6 1,097,190 + Dshi_1063 0.77 +0.4 1,097,193 - Dshi_1063 0.78 +0.4 1,097,231 + +2.7 1,097,231 - -0.6 1,097,822 - +0.4 1,097,896 + -1.1 1,097,913 - +0.4 1,098,012 + +2.4 1,098,014 + -1.4 1,098,091 + +0.4 1,098,096 - -1.9 1,098,098 - +0.5 1,098,123 + +0.4 1,098,133 + +0.4 1,098,141 - Dshi_1065 0.13 -1.1 1,098,194 + Dshi_1065 0.32 +0.4 1,098,247 - Dshi_1065 0.52 -0.3 1,098,350 + -1.3 1,098,500 + -0.4 1,098,540 + Dshi_1066 0.19 +0.4 1,098,548 - Dshi_1066 0.21 -2.2 1,098,563 - Dshi_1066 0.24 -1.2 1,098,563 - Dshi_1066 0.24 -0.2 1,098,605 - Dshi_1066 0.34 -1.7 1,098,656 + Dshi_1066 0.46 -2.3
Or see this region's nucleotide sequence