Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0692 and Dshi_0693 overlap by 4 nucleotides Dshi_0693 and Dshi_0694 are separated by 157 nucleotides Dshi_0694 and Dshi_0695 overlap by 4 nucleotides
Dshi_0692: Dshi_0692 - 5-oxoprolinase (ATP-hydrolyzing) (RefSeq), at 698,375 to 700,072
_0692
Dshi_0693: Dshi_0693 - 4-oxalocrotonate tautomerase (RefSeq), at 700,069 to 700,644
_0693
Dshi_0694: Dshi_0694 - transcriptional regulator, GntR family (RefSeq), at 700,802 to 701,602
_0694
Dshi_0695: Dshi_0695 - 3-isopropylmalate dehydratase, large subunit (RefSeq), at 701,599 to 702,993
_0695
Position (kb) 700
701 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 699.101 kb on + strand, within Dshi_0692 at 699.107 kb on - strand, within Dshi_0692 at 699.117 kb on - strand, within Dshi_0692 at 699.195 kb on + strand, within Dshi_0692 at 699.213 kb on - strand, within Dshi_0692 at 699.517 kb on + strand, within Dshi_0692 at 699.580 kb on - strand, within Dshi_0692 at 699.642 kb on + strand, within Dshi_0692 at 699.642 kb on + strand, within Dshi_0692 at 699.642 kb on + strand, within Dshi_0692 at 699.650 kb on - strand, within Dshi_0692 at 699.653 kb on + strand, within Dshi_0692 at 699.745 kb on - strand, within Dshi_0692 at 699.757 kb on + strand, within Dshi_0692 at 699.860 kb on + strand, within Dshi_0692 at 699.896 kb on - strand, within Dshi_0692 at 699.898 kb on + strand, within Dshi_0692 at 699.908 kb on - strand at 699.974 kb on - strand at 700.054 kb on - strand at 700.204 kb on - strand, within Dshi_0693 at 700.230 kb on - strand, within Dshi_0693 at 700.358 kb on + strand, within Dshi_0693 at 700.429 kb on - strand, within Dshi_0693 at 700.454 kb on - strand, within Dshi_0693 at 700.565 kb on + strand, within Dshi_0693 at 700.592 kb on + strand at 700.606 kb on + strand at 700.618 kb on + strand at 700.698 kb on + strand at 700.729 kb on + strand at 700.865 kb on - strand at 700.901 kb on + strand, within Dshi_0694 at 701.054 kb on + strand, within Dshi_0694 at 701.301 kb on + strand, within Dshi_0694 at 701.320 kb on - strand, within Dshi_0694 at 701.332 kb on - strand, within Dshi_0694 at 701.385 kb on + strand, within Dshi_0694 at 701.425 kb on + strand, within Dshi_0694 at 701.482 kb on - strand, within Dshi_0694 at 701.482 kb on - strand, within Dshi_0694 at 701.525 kb on + strand at 701.525 kb on + strand at 701.525 kb on + strand at 701.541 kb on + strand at 701.603 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 699,101 + Dshi_0692 0.43 +2.8 699,107 - Dshi_0692 0.43 -0.6 699,117 - Dshi_0692 0.44 +2.0 699,195 + Dshi_0692 0.48 +0.1 699,213 - Dshi_0692 0.49 -1.1 699,517 + Dshi_0692 0.67 +2.0 699,580 - Dshi_0692 0.71 +1.2 699,642 + Dshi_0692 0.75 -0.6 699,642 + Dshi_0692 0.75 -0.2 699,642 + Dshi_0692 0.75 +0.1 699,650 - Dshi_0692 0.75 +1.0 699,653 + Dshi_0692 0.75 +0.0 699,745 - Dshi_0692 0.81 +0.4 699,757 + Dshi_0692 0.81 -0.8 699,860 + Dshi_0692 0.87 +0.4 699,896 - Dshi_0692 0.90 -1.1 699,898 + Dshi_0692 0.90 -1.6 699,908 - -2.3 699,974 - -1.1 700,054 - -1.1 700,204 - Dshi_0693 0.23 +0.4 700,230 - Dshi_0693 0.28 -0.6 700,358 + Dshi_0693 0.50 +2.2 700,429 - Dshi_0693 0.62 +0.4 700,454 - Dshi_0693 0.67 +1.4 700,565 + Dshi_0693 0.86 -1.3 700,592 + +0.4 700,606 + +2.0 700,618 + +3.4 700,698 + +1.8 700,729 + +3.0 700,865 - -0.7 700,901 + Dshi_0694 0.12 +0.5 701,054 + Dshi_0694 0.31 -1.9 701,301 + Dshi_0694 0.62 +0.3 701,320 - Dshi_0694 0.65 +1.5 701,332 - Dshi_0694 0.66 +2.3 701,385 + Dshi_0694 0.73 +0.5 701,425 + Dshi_0694 0.78 -1.1 701,482 - Dshi_0694 0.85 +1.5 701,482 - Dshi_0694 0.85 -1.9 701,525 + -0.4 701,525 + -2.9 701,525 + -1.2 701,541 + -1.1 701,603 - +0.3
Or see this region's nucleotide sequence