Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0415 and Dshi_0416 are separated by 345 nucleotides Dshi_0416 and Dshi_0417 are separated by 83 nucleotides Dshi_0417 and Dshi_0418 are separated by 74 nucleotides
Dshi_0415: Dshi_0415 - hypothetical protein (RefSeq), at 409,020 to 409,262
_0415
Dshi_0416: Dshi_0416 - protein of unknown function DUF1127 (RefSeq), at 409,608 to 409,919
_0416
Dshi_0417: Dshi_0417 - hypothetical protein (RefSeq), at 410,003 to 410,209
_0417
Dshi_0418: Dshi_0418 - UspA domain protein (RefSeq), at 410,284 to 411,177
_0418
Position (kb)
410
411 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 409.015 kb on + strand at 409.025 kb on - strand at 409.073 kb on - strand, within Dshi_0415 at 409.074 kb on - strand, within Dshi_0415 at 409.107 kb on - strand, within Dshi_0415 at 409.135 kb on + strand, within Dshi_0415 at 409.143 kb on - strand, within Dshi_0415 at 409.155 kb on + strand, within Dshi_0415 at 409.210 kb on + strand, within Dshi_0415 at 409.218 kb on - strand, within Dshi_0415 at 409.220 kb on + strand, within Dshi_0415 at 409.228 kb on - strand, within Dshi_0415 at 409.232 kb on + strand at 409.232 kb on + strand, within Dshi_0415 at 409.235 kb on - strand, within Dshi_0415 at 409.240 kb on - strand at 409.242 kb on + strand at 409.249 kb on - strand at 409.264 kb on - strand at 409.264 kb on - strand at 409.277 kb on - strand at 409.291 kb on - strand at 409.291 kb on - strand at 409.291 kb on - strand at 409.298 kb on - strand at 409.298 kb on - strand at 409.298 kb on - strand at 409.326 kb on + strand at 409.350 kb on + strand at 409.396 kb on + strand at 409.399 kb on + strand at 409.407 kb on + strand at 409.429 kb on + strand at 409.429 kb on + strand at 409.429 kb on + strand at 409.437 kb on - strand at 409.448 kb on - strand at 409.448 kb on - strand at 409.449 kb on + strand at 409.511 kb on + strand at 409.531 kb on - strand at 409.566 kb on + strand at 409.572 kb on - strand at 409.587 kb on - strand at 409.587 kb on - strand at 409.597 kb on - strand at 409.600 kb on - strand at 409.624 kb on - strand at 409.676 kb on - strand, within Dshi_0416 at 409.680 kb on + strand, within Dshi_0416 at 409.688 kb on - strand, within Dshi_0416 at 409.688 kb on - strand, within Dshi_0416 at 409.901 kb on - strand at 409.928 kb on - strand at 409.973 kb on + strand at 409.991 kb on - strand at 409.996 kb on - strand at 410.006 kb on - strand at 410.042 kb on + strand, within Dshi_0417 at 410.044 kb on - strand, within Dshi_0417 at 410.055 kb on - strand, within Dshi_0417 at 410.067 kb on - strand, within Dshi_0417 at 410.103 kb on - strand, within Dshi_0417 at 410.109 kb on - strand, within Dshi_0417 at 410.169 kb on - strand, within Dshi_0417 at 410.326 kb on - strand at 410.328 kb on + strand at 410.336 kb on - strand at 410.461 kb on + strand, within Dshi_0418 at 410.501 kb on + strand, within Dshi_0418 at 410.509 kb on - strand, within Dshi_0418 at 410.551 kb on - strand, within Dshi_0418 at 410.648 kb on + strand, within Dshi_0418 at 410.649 kb on + strand, within Dshi_0418 at 410.708 kb on - strand, within Dshi_0418 at 410.776 kb on + strand, within Dshi_0418 at 410.791 kb on - strand, within Dshi_0418 at 410.827 kb on + strand, within Dshi_0418 at 410.835 kb on - strand, within Dshi_0418 at 410.879 kb on + strand, within Dshi_0418 at 411.024 kb on - strand, within Dshi_0418 at 411.106 kb on + strand at 411.130 kb on + strand at 411.189 kb on + strand at 411.189 kb on + strand at 411.197 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 409,015 + +0.4 409,025 - -0.0 409,073 - Dshi_0415 0.22 -1.3 409,074 - Dshi_0415 0.22 +0.8 409,107 - Dshi_0415 0.36 -1.1 409,135 + Dshi_0415 0.47 +0.4 409,143 - Dshi_0415 0.51 -1.1 409,155 + Dshi_0415 0.56 -0.7 409,210 + Dshi_0415 0.78 +2.6 409,218 - Dshi_0415 0.81 -1.3 409,220 + Dshi_0415 0.82 -1.7 409,228 - Dshi_0415 0.86 -1.7 409,232 + +1.3 409,232 + Dshi_0415 0.87 -2.1 409,235 - Dshi_0415 0.88 -0.5 409,240 - -0.3 409,242 + -0.9 409,249 - +1.8 409,264 - +0.3 409,264 - -2.7 409,277 - -0.9 409,291 - -1.6 409,291 - -1.1 409,291 - -2.1 409,298 - +0.4 409,298 - -0.6 409,298 - -0.5 409,326 + -0.6 409,350 + +0.8 409,396 + -0.6 409,399 + -0.0 409,407 + +0.3 409,429 + -1.7 409,429 + +1.3 409,429 + -1.2 409,437 - -0.2 409,448 - -0.7 409,448 - -1.2 409,449 + -2.2 409,511 + -1.3 409,531 - +0.2 409,566 + +0.2 409,572 - -0.6 409,587 - +0.0 409,587 - -1.3 409,597 - -0.4 409,600 - +1.8 409,624 - -0.1 409,676 - Dshi_0416 0.22 -2.3 409,680 + Dshi_0416 0.23 -2.1 409,688 - Dshi_0416 0.26 -0.7 409,688 - Dshi_0416 0.26 +0.1 409,901 - -0.3 409,928 - +2.0 409,973 + +2.4 409,991 - -1.9 409,996 - +1.8 410,006 - -0.9 410,042 + Dshi_0417 0.19 -1.1 410,044 - Dshi_0417 0.20 +1.8 410,055 - Dshi_0417 0.25 -0.4 410,067 - Dshi_0417 0.31 -1.1 410,103 - Dshi_0417 0.48 +2.6 410,109 - Dshi_0417 0.51 +2.4 410,169 - Dshi_0417 0.80 +0.6 410,326 - -0.4 410,328 + +0.5 410,336 - +1.4 410,461 + Dshi_0418 0.20 -1.3 410,501 + Dshi_0418 0.24 -1.5 410,509 - Dshi_0418 0.25 -1.3 410,551 - Dshi_0418 0.30 -2.9 410,648 + Dshi_0418 0.41 -1.8 410,649 + Dshi_0418 0.41 +0.2 410,708 - Dshi_0418 0.47 -1.3 410,776 + Dshi_0418 0.55 -3.9 410,791 - Dshi_0418 0.57 -0.4 410,827 + Dshi_0418 0.61 +3.8 410,835 - Dshi_0418 0.62 -1.0 410,879 + Dshi_0418 0.67 -2.3 411,024 - Dshi_0418 0.83 -0.3 411,106 + -1.3 411,130 + -2.0 411,189 + +0.4 411,189 + +1.8 411,197 + -0.8
Or see this region's nucleotide sequence