Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0072 and Dshi_0073 are separated by 14 nucleotides Dshi_0073 and Dshi_0074 are separated by 99 nucleotides Dshi_0074 and Dshi_0075 are separated by 263 nucleotides Dshi_0075 and Dshi_0076 are separated by 392 nucleotides
Dshi_0072: Dshi_0072 - putative anti-sigma regulatory factor, serine/threonine protein kinase (RefSeq), at 72,738 to 73,247
_0072
Dshi_0073: Dshi_0073 - anti-sigma-factor antagonist (RefSeq), at 73,262 to 73,594
_0073
Dshi_0074: Dshi_0074 - acetyl-CoA acetyltransferase (RefSeq), at 73,694 to 74,872
_0074
Dshi_0075: Dshi_0075 - hypothetical protein (RefSeq), at 75,136 to 75,378
_0075
Dshi_0076: Dshi_0076 - hypothetical protein (RefSeq), at 75,771 to 76,007
_0076
Position (kb)
73
74
75 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 72.756 kb on + strand at 72.759 kb on - strand at 72.855 kb on - strand, within Dshi_0072 at 72.861 kb on - strand, within Dshi_0072 at 72.902 kb on + strand, within Dshi_0072 at 72.903 kb on + strand, within Dshi_0072 at 72.908 kb on + strand, within Dshi_0072 at 72.928 kb on + strand, within Dshi_0072 at 73.005 kb on - strand, within Dshi_0072 at 73.041 kb on - strand, within Dshi_0072 at 73.041 kb on - strand, within Dshi_0072 at 73.046 kb on - strand, within Dshi_0072 at 73.056 kb on - strand, within Dshi_0072 at 73.081 kb on - strand, within Dshi_0072 at 73.099 kb on + strand, within Dshi_0072 at 73.099 kb on + strand, within Dshi_0072 at 73.117 kb on - strand, within Dshi_0072 at 73.120 kb on + strand, within Dshi_0072 at 73.157 kb on - strand, within Dshi_0072 at 73.177 kb on + strand, within Dshi_0072 at 73.210 kb on + strand at 73.210 kb on + strand at 73.210 kb on - strand at 73.212 kb on + strand at 73.218 kb on - strand at 73.392 kb on - strand, within Dshi_0073 at 73.428 kb on - strand, within Dshi_0073 at 73.454 kb on + strand, within Dshi_0073 at 73.455 kb on - strand, within Dshi_0073 at 73.469 kb on + strand, within Dshi_0073 at 73.496 kb on + strand, within Dshi_0073 at 73.538 kb on + strand, within Dshi_0073 at 73.605 kb on + strand at 73.627 kb on + strand at 73.627 kb on + strand at 73.629 kb on - strand at 73.635 kb on - strand at 73.732 kb on + strand at 73.777 kb on + strand at 73.964 kb on + strand, within Dshi_0074 at 73.972 kb on - strand, within Dshi_0074 at 74.141 kb on - strand, within Dshi_0074 at 74.150 kb on + strand, within Dshi_0074 at 74.152 kb on - strand, within Dshi_0074 at 74.216 kb on + strand, within Dshi_0074 at 74.224 kb on - strand, within Dshi_0074 at 74.373 kb on - strand, within Dshi_0074 at 74.398 kb on - strand, within Dshi_0074 at 74.482 kb on + strand, within Dshi_0074 at 74.482 kb on + strand, within Dshi_0074 at 74.498 kb on - strand, within Dshi_0074 at 74.537 kb on + strand, within Dshi_0074 at 74.734 kb on - strand, within Dshi_0074 at 74.784 kb on + strand at 74.784 kb on - strand at 74.976 kb on + strand at 74.976 kb on + strand at 74.986 kb on + strand at 75.102 kb on + strand at 75.119 kb on + strand at 75.191 kb on - strand, within Dshi_0075 at 75.246 kb on + strand, within Dshi_0075 at 75.246 kb on + strand, within Dshi_0075 at 75.259 kb on - strand, within Dshi_0075 at 75.278 kb on - strand, within Dshi_0075 at 75.293 kb on - strand, within Dshi_0075 at 75.333 kb on - strand, within Dshi_0075 at 75.411 kb on - strand at 75.418 kb on - strand at 75.421 kb on - strand at 75.451 kb on + strand at 75.459 kb on - strand at 75.502 kb on - strand at 75.508 kb on + strand at 75.508 kb on + strand at 75.508 kb on + strand at 75.508 kb on + strand at 75.553 kb on + strand at 75.575 kb on + strand at 75.583 kb on - strand at 75.685 kb on - strand at 75.807 kb on + strand, within Dshi_0076 at 75.832 kb on + strand, within Dshi_0076 at 75.837 kb on + strand, within Dshi_0076 at 75.840 kb on - strand, within Dshi_0076 at 75.845 kb on - strand, within Dshi_0076
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 72,756 + +1.6 72,759 - -1.4 72,855 - Dshi_0072 0.23 -1.7 72,861 - Dshi_0072 0.24 -0.4 72,902 + Dshi_0072 0.32 +0.9 72,903 + Dshi_0072 0.32 +2.6 72,908 + Dshi_0072 0.33 -1.0 72,928 + Dshi_0072 0.37 +0.3 73,005 - Dshi_0072 0.52 -0.8 73,041 - Dshi_0072 0.59 -2.6 73,041 - Dshi_0072 0.59 -1.1 73,046 - Dshi_0072 0.60 -0.8 73,056 - Dshi_0072 0.62 +2.2 73,081 - Dshi_0072 0.67 -1.2 73,099 + Dshi_0072 0.71 +2.9 73,099 + Dshi_0072 0.71 +1.9 73,117 - Dshi_0072 0.74 -1.7 73,120 + Dshi_0072 0.75 -0.9 73,157 - Dshi_0072 0.82 +0.9 73,177 + Dshi_0072 0.86 -2.1 73,210 + -2.6 73,210 + -0.4 73,210 - -1.0 73,212 + +0.6 73,218 - +0.6 73,392 - Dshi_0073 0.39 +3.2 73,428 - Dshi_0073 0.50 +2.9 73,454 + Dshi_0073 0.58 +2.6 73,455 - Dshi_0073 0.58 +1.6 73,469 + Dshi_0073 0.62 -1.7 73,496 + Dshi_0073 0.70 -1.0 73,538 + Dshi_0073 0.83 -3.0 73,605 + +2.0 73,627 + +0.0 73,627 + +0.6 73,629 - +1.6 73,635 - -2.6 73,732 + -1.0 73,777 + -0.4 73,964 + Dshi_0074 0.23 -0.4 73,972 - Dshi_0074 0.24 +0.6 74,141 - Dshi_0074 0.38 -1.0 74,150 + Dshi_0074 0.39 +0.6 74,152 - Dshi_0074 0.39 -0.4 74,216 + Dshi_0074 0.44 -2.2 74,224 - Dshi_0074 0.45 +2.6 74,373 - Dshi_0074 0.58 +0.1 74,398 - Dshi_0074 0.60 +0.7 74,482 + Dshi_0074 0.67 +1.9 74,482 + Dshi_0074 0.67 -1.4 74,498 - Dshi_0074 0.68 -0.1 74,537 + Dshi_0074 0.72 +0.2 74,734 - Dshi_0074 0.88 -0.6 74,784 + +0.0 74,784 - +2.9 74,976 + +0.8 74,976 + +0.2 74,986 + -1.6 75,102 + -0.3 75,119 + -1.8 75,191 - Dshi_0075 0.23 +0.2 75,246 + Dshi_0075 0.45 +0.2 75,246 + Dshi_0075 0.45 -2.6 75,259 - Dshi_0075 0.51 +1.6 75,278 - Dshi_0075 0.58 +1.6 75,293 - Dshi_0075 0.65 -3.2 75,333 - Dshi_0075 0.81 -2.0 75,411 - +0.0 75,418 - +1.2 75,421 - -0.4 75,451 + -2.6 75,459 - -0.1 75,502 - -0.5 75,508 + -0.6 75,508 + -1.6 75,508 + -0.6 75,508 + +0.6 75,553 + -0.2 75,575 + +1.6 75,583 - +0.6 75,685 - +1.4 75,807 + Dshi_0076 0.15 -2.2 75,832 + Dshi_0076 0.26 +0.9 75,837 + Dshi_0076 0.28 +0.8 75,840 - Dshi_0076 0.29 +0.8 75,845 - Dshi_0076 0.31 -0.6
Or see this region's nucleotide sequence