Experiment: m.b. nitrate 300 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_0041 and Dshi_0042 overlap by 4 nucleotides Dshi_0042 and Dshi_0043 are separated by 86 nucleotides Dshi_0043 and Dshi_0044 are separated by 300 nucleotides Dshi_0044 and Dshi_0045 are separated by 6 nucleotides
Dshi_0041: Dshi_0041 - hypothetical protein (RefSeq), at 42,669 to 43,163
_0041
Dshi_0042: Dshi_0042 - alanine racemase domain protein (RefSeq), at 43,160 to 43,816
_0042
Dshi_0043: Dshi_0043 - hypothetical protein (RefSeq), at 43,903 to 44,862
_0043
Dshi_0044: Dshi_0044 - hypothetical protein (RefSeq), at 45,163 to 45,633
_0044
Dshi_0045: Dshi_0045 - UspA domain protein (RefSeq), at 45,640 to 46,014
_0045
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 42.903 kb on + strand, within Dshi_0041 at 42.916 kb on + strand, within Dshi_0041 at 43.007 kb on - strand, within Dshi_0041 at 43.107 kb on + strand, within Dshi_0041 at 43.168 kb on + strand at 43.333 kb on + strand, within Dshi_0042 at 43.366 kb on + strand, within Dshi_0042 at 43.470 kb on - strand, within Dshi_0042 at 43.501 kb on + strand, within Dshi_0042 at 43.698 kb on + strand, within Dshi_0042 at 43.782 kb on + strand at 43.782 kb on + strand at 45.114 kb on + strand at 45.122 kb on - strand at 45.156 kb on - strand at 45.156 kb on - strand at 45.199 kb on - strand at 45.204 kb on - strand at 45.235 kb on - strand, within Dshi_0044 at 45.235 kb on - strand, within Dshi_0044 at 45.333 kb on - strand, within Dshi_0044 at 45.360 kb on - strand, within Dshi_0044 at 45.430 kb on - strand, within Dshi_0044 at 45.444 kb on - strand, within Dshi_0044 at 45.451 kb on + strand, within Dshi_0044 at 45.473 kb on - strand, within Dshi_0044 at 45.495 kb on - strand, within Dshi_0044 at 45.540 kb on + strand, within Dshi_0044 at 45.540 kb on + strand, within Dshi_0044 at 45.673 kb on + strand at 45.683 kb on + strand, within Dshi_0045 at 45.691 kb on - strand, within Dshi_0045 at 45.712 kb on + strand, within Dshi_0045 at 45.760 kb on - strand, within Dshi_0045 at 45.783 kb on - strand, within Dshi_0045
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 300 mM remove 42,903 + Dshi_0041 0.47 +1.2 42,916 + Dshi_0041 0.50 -0.4 43,007 - Dshi_0041 0.68 +1.6 43,107 + Dshi_0041 0.88 +0.3 43,168 + +2.4 43,333 + Dshi_0042 0.26 +1.6 43,366 + Dshi_0042 0.31 -3.0 43,470 - Dshi_0042 0.47 -0.4 43,501 + Dshi_0042 0.52 -0.6 43,698 + Dshi_0042 0.82 +1.9 43,782 + +0.2 43,782 + +3.2 45,114 + -1.0 45,122 - -1.2 45,156 - -0.4 45,156 - -2.2 45,199 - -1.4 45,204 - +0.6 45,235 - Dshi_0044 0.15 +0.2 45,235 - Dshi_0044 0.15 -1.2 45,333 - Dshi_0044 0.36 +0.6 45,360 - Dshi_0044 0.42 +2.9 45,430 - Dshi_0044 0.57 -0.5 45,444 - Dshi_0044 0.60 +0.9 45,451 + Dshi_0044 0.61 +0.6 45,473 - Dshi_0044 0.66 +0.9 45,495 - Dshi_0044 0.70 -1.5 45,540 + Dshi_0044 0.80 +0.4 45,540 + Dshi_0044 0.80 -1.0 45,673 + -1.3 45,683 + Dshi_0045 0.11 -0.1 45,691 - Dshi_0045 0.14 -0.8 45,712 + Dshi_0045 0.19 -1.6 45,760 - Dshi_0045 0.32 -0.1 45,783 - Dshi_0045 0.38 +0.6
Or see this region's nucleotide sequence