Experiment: L-Proline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_tRNA19 and HP15_1209 are separated by 129 nucleotides HP15_1209 and HP15_1210 overlap by 14 nucleotides HP15_1210 and HP15_1211 are separated by 5 nucleotides
HP15_tRNA19: HP15_tRNA19 - tRNA-Arg, at 1,255,238 to 1,255,314
_tRNA19
HP15_1209: HP15_1209 - glycosyltransferase involved in cell wall biogenesis, at 1,255,444 to 1,256,109
_1209
HP15_1210: HP15_1210 - conserved hypothetical protein, at 1,256,096 to 1,256,728
_1210
HP15_1211: HP15_1211 - protease III precursor, at 1,256,734 to 1,259,556
_1211
Position (kb)
1255
1256
1257 Strain fitness (log2 ratio)
-1
0
1 at 1254.448 kb on - strand at 1254.601 kb on + strand at 1254.663 kb on + strand at 1254.762 kb on - strand at 1254.873 kb on + strand at 1254.936 kb on - strand at 1255.010 kb on + strand at 1255.058 kb on - strand at 1255.070 kb on - strand at 1255.173 kb on - strand at 1255.235 kb on + strand at 1255.372 kb on - strand at 1255.395 kb on - strand at 1255.552 kb on - strand, within HP15_1209 at 1255.644 kb on + strand, within HP15_1209 at 1255.692 kb on - strand, within HP15_1209 at 1255.946 kb on + strand, within HP15_1209 at 1256.146 kb on + strand at 1256.245 kb on + strand, within HP15_1210 at 1256.332 kb on + strand, within HP15_1210 at 1256.358 kb on + strand, within HP15_1210 at 1256.366 kb on - strand, within HP15_1210 at 1256.422 kb on - strand, within HP15_1210 at 1256.516 kb on + strand, within HP15_1210 at 1256.602 kb on - strand, within HP15_1210 at 1256.693 kb on + strand at 1256.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline remove 1,254,448 - -0.5 1,254,601 + -0.1 1,254,663 + +1.0 1,254,762 - +0.1 1,254,873 + -0.1 1,254,936 - -0.0 1,255,010 + +0.9 1,255,058 - -0.0 1,255,070 - +0.1 1,255,173 - +0.5 1,255,235 + +0.6 1,255,372 - -0.4 1,255,395 - -0.1 1,255,552 - HP15_1209 0.16 +0.2 1,255,644 + HP15_1209 0.30 -0.4 1,255,692 - HP15_1209 0.37 +0.5 1,255,946 + HP15_1209 0.75 -1.5 1,256,146 + +0.3 1,256,245 + HP15_1210 0.24 +0.7 1,256,332 + HP15_1210 0.37 +0.6 1,256,358 + HP15_1210 0.41 +0.1 1,256,366 - HP15_1210 0.43 +0.3 1,256,422 - HP15_1210 0.52 -0.2 1,256,516 + HP15_1210 0.66 +0.2 1,256,602 - HP15_1210 0.80 -0.0 1,256,693 + -0.0 1,256,729 - +0.4
Or see this region's nucleotide sequence