Experiment: L-Threonine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2363 and Shewana3_R0100 are separated by 282 nucleotides Shewana3_R0100 and Shewana3_R0101 are separated by 56 nucleotides Shewana3_R0101 and Shewana3_2364 are separated by 165 nucleotides Shewana3_2364 and Shewana3_2365 are separated by 358 nucleotides
Shewana3_2363: Shewana3_2363 - cytochrome c552 (RefSeq), at 2,840,377 to 2,841,810
_2363
Shewana3_R0100: Shewana3_R0100 - tRNA-Asp (RefSeq), at 2,842,093 to 2,842,169
_R0100
Shewana3_R0101: Shewana3_R0101 - tRNA-Asp (RefSeq), at 2,842,226 to 2,842,302
_R0101
Shewana3_2364: Shewana3_2364 - hypothetical protein (RefSeq), at 2,842,468 to 2,843,652
_2364
Shewana3_2365: Shewana3_2365 - DNA polymerase III, epsilon subunit (RefSeq), at 2,844,011 to 2,844,739
_2365
Position (kb)
2842
2843
2844 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 2841.602 kb on + strand, within Shewana3_2363 at 2841.620 kb on - strand, within Shewana3_2363 at 2841.856 kb on + strand at 2842.574 kb on - strand at 2842.755 kb on + strand, within Shewana3_2364 at 2842.910 kb on - strand, within Shewana3_2364 at 2842.910 kb on - strand, within Shewana3_2364 at 2842.925 kb on - strand, within Shewana3_2364 at 2843.145 kb on - strand, within Shewana3_2364 at 2843.155 kb on - strand, within Shewana3_2364 at 2843.392 kb on - strand, within Shewana3_2364 at 2843.462 kb on + strand, within Shewana3_2364 at 2843.837 kb on + strand at 2843.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (C) remove 2,841,602 + Shewana3_2363 0.85 +0.7 2,841,620 - Shewana3_2363 0.87 -2.0 2,841,856 + -0.5 2,842,574 - -2.0 2,842,755 + Shewana3_2364 0.24 +2.9 2,842,910 - Shewana3_2364 0.37 +5.8 2,842,910 - Shewana3_2364 0.37 +4.0 2,842,925 - Shewana3_2364 0.39 +3.3 2,843,145 - Shewana3_2364 0.57 +2.5 2,843,155 - Shewana3_2364 0.58 +7.1 2,843,392 - Shewana3_2364 0.78 +2.6 2,843,462 + Shewana3_2364 0.84 +2.8 2,843,837 + +5.7 2,843,837 + +5.5
Or see this region's nucleotide sequence