Strain Fitness in Shewanella sp. ANA-3 around Shewana3_1350

Experiment: L-Threonine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfliM and fliN are separated by 12 nucleotidesfliN and Shewana3_1351 are separated by 33 nucleotidesShewana3_1351 and fliP overlap by 4 nucleotides Shewana3_1349: fliM - flagellar motor switch protein FliM (RefSeq), at 1,562,900 to 1,563,931 fliM Shewana3_1350: fliN - flagellar motor switch protein (RefSeq), at 1,563,944 to 1,564,318 fliN Shewana3_1351: Shewana3_1351 - flagellar biosynthesis protein, FliO (RefSeq), at 1,564,352 to 1,564,759 _1351 Shewana3_1352: fliP - flagellar biosynthesis protein FliP (RefSeq), at 1,564,756 to 1,565,502 fliP Position (kb) 1563 1564 1565Strain fitness (log2 ratio) -1 0 1 2 3 4 5 6 7 8at 1563.175 kb on + strand, within fliMat 1563.241 kb on - strand, within fliMat 1563.608 kb on - strand, within fliMat 1563.635 kb on + strand, within fliMat 1563.692 kb on + strand, within fliMat 1563.961 kb on - strandat 1563.961 kb on - strandat 1563.961 kb on - strandat 1564.005 kb on - strand, within fliNat 1564.005 kb on - strand, within fliNat 1564.013 kb on - strand, within fliNat 1564.076 kb on + strand, within fliNat 1564.171 kb on - strand, within fliNat 1564.241 kb on + strand, within fliNat 1564.337 kb on + strandat 1564.337 kb on + strandat 1564.349 kb on + strandat 1564.415 kb on + strand, within Shewana3_1351at 1564.558 kb on + strand, within Shewana3_1351at 1564.731 kb on + strandat 1564.783 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (C)
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1,563,175 + fliM Shewana3_1349 0.27 +8.7
1,563,241 - fliM Shewana3_1349 0.33 +5.1
1,563,608 - fliM Shewana3_1349 0.69 +7.3
1,563,635 + fliM Shewana3_1349 0.71 +8.1
1,563,692 + fliM Shewana3_1349 0.77 +8.0
1,563,961 - +4.6
1,563,961 - +8.3
1,563,961 - +7.4
1,564,005 - fliN Shewana3_1350 0.16 +8.5
1,564,005 - fliN Shewana3_1350 0.16 +7.1
1,564,013 - fliN Shewana3_1350 0.18 +7.6
1,564,076 + fliN Shewana3_1350 0.35 +8.5
1,564,171 - fliN Shewana3_1350 0.61 +3.9
1,564,241 + fliN Shewana3_1350 0.79 +8.5
1,564,337 + +4.7
1,564,337 + -0.0
1,564,349 + +3.5
1,564,415 + Shewana3_1351 0.15 +4.3
1,564,558 + Shewana3_1351 0.50 +3.9
1,564,731 + -0.0
1,564,783 - +3.2

Or see this region's nucleotide sequence